High urinary excretion of lipid peroxidation-derived DNA damage in patients with cancer-prone liver diseases

Objective: The objective of this research was to evaluate oxidative stress status in children with β-thalassemia major.Methods: Our study was conducted in children with β-thalassemia aged from 5 to 15 years. Investigate the urinary excretion of human 8-oxo-7,8- dihydro-2′-deoxyguanosine, which will be analyzed by enzyme-linked immunosorbent assay. To investigate serum levels of antioxidant enzymes include glutathione s-transferase (GST) and catalase (CAT).Results: We found a significant elevation of the urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine level with p=0.001 compared to control group, a significant reduction of both GST and CAT p=0.05 and 0.03, respectively, compared to control group. There was a significant negative correlation between urinary 8-oxo-7,8-dihydro-2′-deoxyguanisine and CAT level, r=−0.378, p=0.016, hemoglobin - r=−0.610, p=0.001, hematocrit (%) - r=−0.478, p=0.002, while a significant positive correlation between urinary 8-oxo-7,8-dihydro-2′-deoxyguanisine and alanine aminotransferase - r=0.547, p=0.001, and serum ferritin - r=0.391, p=0.013. There was a significant negative correlation between CAT and serum ferritin - r=−0.320, p=0.44.Conclusion: We conclude that the strongly increased urinary excretion 8-oxo-7,8=dihydro-2′-deoxyguanisine indicates elevated lipid peroxidation induced DNA damage in internal organs such as the liver. These highly pro mutagenic lesions may contribute to the increased risk of thalassemia patients to develop hepatocellular carcinoma.

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In situ detection of lipid peroxidation and oxidative DNA damage in non-alcoholic fatty liver diseases

Journal of Hepatology ◽

10.1016/s0168-8278(02)00073-9 ◽

2002 ◽

Vol 37 (1) ◽

pp. 56-62 ◽

Cited By ~ 321

Author(s):

Shuichi Seki ◽

Takuya Kitada ◽

Takao Yamada ◽

Hiroki Sakaguchi ◽

Kazuki Nakatani ◽

...

Keyword(s):

Lipid Peroxidation ◽

Dna Damage ◽

Fatty Liver ◽

Liver Diseases ◽

Oxidative Dna Damage ◽

Alcoholic Fatty Liver ◽

In Situ Detection

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Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women

Carcinogenesis ◽

10.1093/carcin/bgi337 ◽

2006 ◽

Vol 27 (7) ◽

pp. 1398-1403 ◽

Cited By ~ 27

Author(s):

Kyoung-Ho Lee ◽

Helmut Bartsch ◽

Jagadeesan Nair ◽

Dong-Ho Yoo ◽

Yun-Chul Hong ◽

...

Keyword(s):

Lipid Peroxidation ◽

Dna Damage ◽

Urinary Excretion ◽

Oxidative Dna Damage ◽

Short Term ◽

Lipid Peroxidation Products ◽

Healthy Women

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In situ detection of lipid peroxidation and oxidative DNA damage in non-alcoholic fatty liver diseases

Journal of Hepatology ◽

10.1016/s0168-8278(02)80938-2 ◽

2002 ◽

Vol 36 ◽

pp. 260

Author(s):

Shuichi Seki ◽

Takuya Kitada ◽

Hiroki Sakaguchi ◽

Takao Yamada ◽

Osuke Muroya ◽

...

Keyword(s):

Lipid Peroxidation ◽

Dna Damage ◽

Fatty Liver ◽

Liver Diseases ◽

Oxidative Dna Damage ◽

Alcoholic Fatty Liver ◽

In Situ Detection

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Acrylamide Exposure and Oxidative DNA Damage, Lipid Peroxidation and Fasting Plasma Glucose Alteration: Association and Mediation Analyses in Chinese Urban Adults

10.2337/figshare.12076134 ◽

2020 ◽

Author(s):

Bin Wang ◽

Weihong Qiu ◽

Shijie Yang ◽

Limin Cao ◽

Chunmei Zhu ◽

...

Keyword(s):

Lipid Peroxidation ◽

Dna Damage ◽

Plasma Glucose ◽

Fasting Plasma Glucose ◽

Oxidative Dna Damage ◽

Adult Population ◽

Prostaglandin F2α ◽

Mediation Analyses ◽

Mediating Role ◽

Fasting Plasma

<a>OBJECTIVE: </a>Acrylamide exposure from daily-consumed food has raised global concern. We aimed to assess the exposure-response relationships of internal acrylamide exposure with oxidative DNA damage, lipid peroxidation and fasting plasma glucose (FPG) alteration, and investigate the mediating role of oxidative DNA damage and lipid peroxidation in the association of internal acrylamide exposure with FPG. RESEARCH DESIGN AND METHODS: FPG and urinary biomarkers of oxidative DNA damage (8-hydroxy-deoxy-guanosine, 8-OHdG), lipid peroxidation (8-iso-prostaglandin-F2α, 8-iso-PGF2α) and acrylamide exposure (N-acetyl-S-(2-carbamoylethyl)-L-cysteine, AAMA; N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine, GAMA) were measured for 3,270 general adults from the Wuhan-Zhuhai cohort. The associations of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG were assessed by linear mixed models. The mediating roles of 8-OHdG and 8-iso-PGF2α were evaluated by mediation analysis. RESULTS: We found significant linear positive dose-response relationships of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG (except GAMA with FPG), and 8-iso-PGF2α with FPG. Each 1-unit increase in log-transformed level of AAMA, ΣUAAM (AAMA+GAMA) or 8-iso-PGF2α was associated with a 0.17-, 0.15- or 0.23-mmol/L increase in FPG, respectively (P or/and P trend<0.05). Each 1% increase in AAMA, GAMA or ΣUAAM was associated with a 0.19%, 0.27% or 0.22% increase in 8-OHdG, respectively, and a 0.40%, 0.48% or 0.44% increase in 8-iso-PGF2α, respectively (P and P trend<0.05). Increased 8-iso-PGF2α rather than 8-OHdG significantly mediated 64.29% and 76.92% of the AAMA and ΣUAAM associated-FPG increases, respectively. CONCLUSIONS: Exposure of general adult population to acrylamide was associated with FPG elevation, oxidative DNA damage and lipid peroxidation, which in turn partly mediated acrylamide-associated FPG elevation.

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