Polymorphisms affecting micro-RNA regulation and associated with the risk of dietary-related cancers: A review from the literature and new evidence for a functional role of rs17281995 (CD86) and rs1051690 (INSR), previously associated with colorectal cancer

Abstract The functional role of microRNA-23a in tumorigenesis has been investigated; however, the exact mechanism of microRNA-23a (miR-23a) in colorectal cancer development has not been fully explored. In the present study, we aimed to investigate the molecular functional role of miR-23a in colorectal carcinogenesis. Quantitative real-time polymerase chain reaction was conducted to investigate the expression level of miR-23a in tissue samples and cell lines (HCT116 and SW480). CCK-8, colony formation and Transwell assay were used to explore the role of miR-23a in cell proliferation and migration. Dual luciferase reporter assay was used to identify the direct binding of miR-23a with its target, MARK1. Western blot analysis was used to analyze the expression level of MARK1, as well as a confirmed miR-23a target gene, MTSS1, in miR-23a-mimic and miR-23a-inhibit groups. Rescue experiments were conducted by overexpression of MARK1 in miR-23a-mimic-transfected cell lines. The results showed that miR-23a was highly expressed in colorectal cancer tissue and cell lines. MiR-23a could promote proliferation and migration of colorectal cancer cell lines. MARK1 was a direct target of miR-23a and the expression level of MARK1 was down-regulated in miR-23a-mimic-transfected cell lines but up-regulated in miR-23a-inhibit-transfected cells. Overexpression of MARK1 could partly reverse the cancer-promoting function of miR-23a. Our results suggested that miR-23a promotes colorectal cancer cell proliferation and migration by mediating the expression of MARK1. MiR-23a may be a potential therapeutic target for colorectal cancer treatment.

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Role of Micro-RNA in Colorectal Cancer Screening

Cirugía Española (English Edition) ◽

10.1016/j.cireng.2014.05.007 ◽

2014 ◽

Vol 92 (10) ◽

pp. 654-658 ◽

Cited By ~ 1

Author(s):

José Antonio Rodríguez-Montes ◽

Pablo Menéndez Sánchez

Keyword(s):

Colorectal Cancer ◽

Cancer Screening ◽

Colorectal Cancer Screening ◽

Micro Rna

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Downregulation of Dual-specificity phosphatase 9 promotes tumor progression and contributes to poor prognosis in colorectal cancer

10.21203/rs.2.23210/v1 ◽

2020 ◽

Author(s):

Zhaoyan Qiu ◽

Ning Liang ◽

Tao Sun ◽

Hongyuan Xue ◽

Tianyu Xie ◽

...

Keyword(s):

Colorectal Cancer ◽

Functional Role ◽

Cpg Island ◽

Functional Study ◽

Dual Specificity Phosphatase ◽

Dual Specificity ◽

Low Expression

Abstract Background Dual-specificity phosphatase 9 (DUSP9) belongs to the dual-specificity protein phosphatase subfamily. Recently, increasing attention has been paid on the role of DUSP9 in a variety of cancers. However, its functional role in tumor development is still unclear, especially in colorectal cancer (CRC). Methods The functional role of DUSP9 in inhibiting the progression of CRC was verified both in vivo and in vitro using colony formation assay, EdU incorporation assay, wound healing assay, nude mice xenograft model, and et al. RNA-seq was performed to assess the gene expression profiling in SW480 cells with DUSP9 stable knockdown and shControl cells. Bisulfite sequencing (BSE) was performed to reveal methylation status of CpG island in promoter of DUSP9. Results DUSP9 was significantly down regulated in tumor tissues compared with peritumor tissues. Moreover, low DUSP9 expression in CRC was closely associated with tumor size, depth of invasion and advanced TNM stage, indicating that DUSP9 may be involved in the progression of CRC. Kaplan–Meier survival analysis showed that the overall survival (OS) and recurrence-free survival (RFS) of patients with low expression of DUSP9 were significantly shorter than that of patients with high expression of DUSP9. Functional study revealed that DUSP9 inhibited tumor migration, invasion and metastasis both in vitro and in vivo . Mechanistically, low expression of DUSP9 in CRC was caused by the upregulation of miR-1246 and hypermethylation status of CpG island in promoter of DUSP9. Conclusion Our findings demonstrate that DUSP9 plays a critical role in the progression of CRC and therapeutic intervention to increase the expression or activity of DUSP9 may be a potential target for CRC treatment in the future.

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The functional role of micro RNA s in alcoholic liver injury

Journal of Cellular and Molecular Medicine ◽

10.1111/jcmm.12223 ◽

2014 ◽

Vol 18 (2) ◽

pp. 197-207 ◽

Cited By ~ 73

Author(s):

Kelly McDaniel ◽

Leonardo Herrera ◽

Tianhao Zhou ◽

Heather Francis ◽

Yuyan Han ◽

...

Keyword(s):

Liver Injury ◽

Functional Role ◽

Micro Rna ◽

Alcoholic Liver Injury

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34P The expression and functional role of Cripto-1 in human colorectal cancer

Annals of Oncology ◽

10.1093/annonc/mdw574.007 ◽

2016 ◽

Vol 27 (suppl_9) ◽

Author(s):

S. Jun ◽

H. Karasawa ◽

T. Suzuki ◽

S. Nakayama ◽

M. Katagiri ◽

...

Keyword(s):

Colorectal Cancer ◽

Functional Role ◽

Human Colorectal Cancer

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34P The expression and functional role of Cripto-1 in human colorectal cancer

Annals of Oncology ◽

10.1016/s0923-7534(21)00194-0 ◽

2016 ◽

Vol 27 ◽

pp. ix10

Author(s):

S. Jun ◽

H. Karasawa ◽

T. Suzuki ◽

S. Nakayama ◽

M. Katagiri ◽

...

Keyword(s):

Colorectal Cancer ◽

Functional Role ◽

Human Colorectal Cancer

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A Review on Role of Nanosensors in Detecting Cellular miRNA Expression in Colorectal Cancer

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200515115723 ◽

2020 ◽

Vol 20 ◽

Author(s):

Koyeli Girigoswami ◽

Agnishwar Girigoswami

Keyword(s):

Colorectal Cancer ◽

General Scheme ◽

High Sensitivity ◽

Micro Rna ◽

Rna Detection ◽

Piezoelectric Detection ◽

Current Scenario ◽

Mirna Detection ◽

Causes Of Deaths

Background: Colorectal cancer (CRC) is one of the leading causes of deaths due to cancers in the world. Early diagnosis with high sensitivity can prevent the CRC progression, thereby reducing the condition of metastasis. Objective: The purpose of this review is (i) to discuss about miRNA based biomarkers responsible for CRC, (ii) to brief on the different methods used for the detection of miRNA in CRC, (iii) to discuss about different nanobiosensors so far found for the accurate detection of miRNAs in CRC using spectrophotometric detection, piezoelectric detection. Method: The keywords for the review were micro RNA detection in inflammation, colorectal cancer, nanotechnology, were searched in PubMed and the relevant papers on the topics of miRNA related to CRC, nanotechnology based biosensors for miRNA detection were sorted and used appropriately for writing the review. Results: The review comprised of a general introduction explaining the current scenario of CRC, the biomarkers used for detection of different cancers, especially CRC and the importance of nanotechnology and a general scheme of a biosensor. The further subsections discussed about the mechanism of CRC progression, role of miRNA in CRC progression and different nanotechnology based biosensors so far investigated for miRNA detection in other diseases, cancer and CRC. A scheme depicting miRNA detection using gold nanoparticles (AuNPs) is also illustrated. Conclusion: This review may give insight into the different nanostructure like AuNPs, quantum dots, silver nanoparticles, MoS2derived nanoparticles etc. based approach for miRNA detetction using biosensors.

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