Biomaterial surface properties modulate in vitro rat calvaria osteoblasts response: Roughness and or chemistry?

Natural sheep surfactant, rabbit surfactant, human surfactant, and surfactant TA were compared for in vitro surface properties and for responses of preterm lambs to treatment. Equivalent amounts of sheep, rabbit, and human surfactants were needed to lower the surface tension to less than 10 dynes/cm, whereas four times less surfactant TA similarly lowered the surface tension. Surface-spreading rates were similar for the surfactants. The surface adsorption of the batch of human surfactant tested was much slower than was adsorption of the other surfactants. Ventilation was significantly improved in all surfactant-treated lambs relative to the control lambs, indicating the general efficacy of the surfactant treatments. Overall, surfactant TA had the best in vitro characteristics, yet the preterm lambs treated at birth with surfactant TA had lower Po2 values and higher ventilatory requirements than did the sheep surfactant-treated lambs. The in vivo responses to rabbit surfactant were intermediate between the responses to sheep surfactant and to surfactant TA. Human surfactant resulted in the least effective clinical response. More of the phosphatidylcholine associated with human surfactant and surfactant TA was lost from the alveoli and lung tissue after four hours of ventilation than was lost from sheep or rabbit surfactant-treated lambs. More intravascular radiolabeled albumin leaked into the alveoli of the surfactant TA-treated lambs than sheep or rabbit surfactant-treated. lambs. The four surfactants also had different sensitivities to the effects on minimum surface tensions of the soluble proteins present in alveolar washes. The study demonstrates that the range of clinical responses was not predictable based on the in vitro surface properties that we measured. The surfactants behaved differently with respect to loss from the lungs and sensitivity to soluble proteins. Factors other than surface properties are important for the in vivo responses to surfactant treatments.

Download Full-text

Natural Architectures for Tissue Engineering and Regenerative Medicine

Journal of Functional Biomaterials ◽

10.3390/jfb11030047 ◽

2020 ◽

Vol 11 (3) ◽

pp. 47

Author(s):

Floris Honig ◽

Steven Vermeulen ◽

Amir A. Zadpoor ◽

Jan de Boer ◽

Lidy E. Fratila-Apachitei

Keyword(s):

Tissue Engineering ◽

Regenerative Medicine ◽

Surface Properties ◽

State Of The Art ◽

Cell Behaviour ◽

Cell Responses ◽

Underlying Mechanisms ◽

Functional Biomaterials

The ability to control the interactions between functional biomaterials and biological systems is of great importance for tissue engineering and regenerative medicine. However, the underlying mechanisms defining the interplay between biomaterial properties and the human body are complex. Therefore, a key challenge is to design biomaterials that mimic the in vivo microenvironment. Over millions of years, nature has produced a wide variety of biological materials optimised for distinct functions, ranging from the extracellular matrix (ECM) for structural and biochemical support of cells to the holy lotus with special wettability for self-cleaning effects. Many of these systems found in biology possess unique surface properties recognised to regulate cell behaviour. Integration of such natural surface properties in biomaterials can bring about novel cell responses in vitro and provide greater insights into the processes occurring at the cell-biomaterial interface. Using natural surfaces as templates for bioinspired design can stimulate progress in the field of regenerative medicine, tissue engineering and biomaterials science. This literature review aims to combine the state-of-the-art knowledge in natural and nature-inspired surfaces, with an emphasis on material properties known to affect cell behaviour.

Download Full-text

Alterations in the surface properties of lung surfactant in the torpid marsupial Sminthopsis crassicaudata

Journal of Applied Physiology ◽

10.1152/jappl.1998.84.1.146 ◽

1998 ◽

Vol 84 (1) ◽

pp. 146-156 ◽

Cited By ~ 21

Author(s):

Olga V. Lopatko ◽

Sandra Orgeig ◽

Christopher B. Daniels ◽

David Palmer

Keyword(s):

Surface Tension ◽

Surface Properties ◽

Surface Activity ◽

Pulmonary Surfactant ◽

Lung Surfactant ◽

Lung Compliance ◽

Equilibrium Surface Tension ◽

Sminthopsis Crassicaudata

Lopatko, Olga V., Sandra Orgeig, Christopher B. Daniels, and David Palmer. Alterations in the surface properties of lung surfactant in the torpid marsupial Sminthopsis crassicaudata. J. Appl. Physiol. 84(1): 146–156, 1998.—Torpor changes the composition of pulmonary surfactant (PS) in the dunnart Sminthopsis crassicaudata [C. Langman, S. Orgeig, and C. B. Daniels. Am. J. Physiol. 271 ( Regulatory Integrative Comp. Physiol. 40): R437–R445, 1996]. Here we investigated the surface activity of PS in vitro. Five micrograms of phospholipid per centimeter squared surface area of whole lavage (from mice or from warm-active, 4-, or 8-h torpid dunnarts) were applied dropwise onto the subphase of a Wilhelmy-Langmuir balance at 20°C and stabilized for 20 min. After 4 h of torpor, the adsorption rate increased, and equilibrium surface tension (STeq), minimal surface tension (STmin), and the %area compression required to achieve STmin decreased, compared with the warm-active group. After 8 h of torpor, STmin decreased [from 5.2 ± 0.3 to 4.1 ± 0.3 (SE) mN/m]; %area compression required to achieve STmindecreased (from 43.4 ± 1.0 to 27.4 ± 0.8); the rate of adsorption decreased; and STeqincreased (from 26.3 ± 0.5 to 38.6 ± 1.3 mN/m). ST-area isotherms of warm-active dunnarts and mice at 20°C had a shoulder on compression and a plateau on expansion. These disappeared on the isotherms of torpid dunnarts. Samples of whole lavage (from warm-active and 8-h torpor groups) containing 100 μg phospholipid/ml were studied by using a captive-bubble surfactometer at 37°C. After 8 h of torpor, STmin increased (from 6.4 ± 0.3 to 9.1 ± 0.3 mN/m) and %area compression decreased in the 2nd (from 88.6 ± 1.7 to 82.1 ± 2.0) and 3rd (from 89.1 ± 0.8 to 84.9 ± 1.8) compression-expansion cycles, compared with warm-active dunnarts. ST-area isotherms of warm-active dunnarts at 37°C did not have a shoulder on compression. This shoulder appeared on the isotherms of torpid dunnarts. In conclusion, there is a strong correlation between in vitro changes in surface activity and in vivo changes in lipid composition of PS during torpor, although static lung compliance remained unchanged (see Langman et al. cited above). Surfactant from torpid animals is more active at 20°C and less active at 37°C than that of warm-active animals, which may represent a respiratory adaptation to low body temperatures of torpid dunnarts.

Download Full-text

Wnt signaling modulates macrophage polarization and is regulated by biomaterial surface properties

Biomaterials ◽

10.1016/j.biomaterials.2020.119920 ◽

2020 ◽

Vol 243 ◽

pp. 119920 ◽

Cited By ~ 2

Author(s):

Jefferson O. Abaricia ◽

Arth H. Shah ◽

Manotri Chaubal ◽

Kelly M. Hotchkiss ◽

Rene Olivares-Navarrete

Keyword(s):

Wnt Signaling ◽

Surface Properties ◽

Macrophage Polarization ◽

Biomaterial Surface

Download Full-text

In Vitro Characterization of Surface Properties Through Living Cells

The Journal of Physical Chemistry Letters ◽

10.1021/jz100742j ◽

2010 ◽

Vol 1 (15) ◽

pp. 2339-2342 ◽

Cited By ~ 15

Author(s):

Mark-Oliver Diesner ◽

Caitlin Howell ◽

Volker Kurz ◽

Dominique Verreault ◽

Patrick Koelsch

Keyword(s):

Surface Properties ◽

Living Cells ◽

In Vitro Characterization

Download Full-text

Analysis of corrosion behaviour and surface properties of plasma-sprayed composite coating of hydroxyapatite–tantalum on biodegradable Mg alloy ZK60

Journal of Composite Materials ◽

10.1177/0021998319839127 ◽

2019 ◽

Vol 53 (19) ◽

pp. 2661-2673 ◽

Cited By ~ 5

Author(s):

Balraj Singh ◽

Gurpreet Singh ◽

Buta Singh Sidhu

Keyword(s):

Corrosion Resistance ◽

Surface Properties ◽

Corrosion Behaviour ◽

Surface Hardness ◽

Mg Alloy ◽

Hydroxyapatite Coating ◽

Hydroxyapatite Coatings ◽

Plasma Sprayed ◽

Incremental Increase

Magnesium (Mg) and its alloys are promising candidates for biodegradable bio-implants. However, the excessive corrosion in the physiological environment and subsequent decline in the mechanical integrity of Mg and its alloys have limited their utility as biomaterials. In the present study, an attempt has been made to improve the corrosion resistance of Mg alloy ZK60 plasma sprayed with tantalum (Ta)-reinforced hydroxyapatite coating. The experiment was conducted with three varied levels, i.e. 10, 20 and 30 weight percent (wt%) of Ta-content in hydroxyapatite coating. The coatings were characterized and in vitro corrosion behaviour was investigated by electrochemical measurements in Ringer's solution along with the analysis of surface properties. The corrosion resistance of the Mg alloy increased with the incremental increase in Ta reinforcement in hydroxyapatite coating. An increase in the protection efficiency was analysed for the Ta-reinforced hydroxyapatite coatings (∼10%, 18% and 23% for hydroxyapatite-10Ta, hydroxyapatite-20Ta and hydroxyapatite-30Ta, respectively) as compared to the pure hydroxyapatite coating. The hydroxyapatite coating effectively increased the surface hardness of the Mg alloy and Ta reinforcement further enhanced it. Surface roughness decreased with the incremental increase in Ta-content in hydroxyapatite coating. Wettability analysis revealed the hydrophilic nature of pure hydroxyapatite and Ta-reinforced hydroxyapatite coatings. The results of the study suggest that the proposed Ta reinforcement in hydroxyapatite is potentially important for biodegradable Mg bio-implants.

Download Full-text