scholarly journals Wnt signaling modulates macrophage polarization and is regulated by biomaterial surface properties

Biomaterials ◽  
2020 ◽  
Vol 243 ◽  
pp. 119920 ◽  
Author(s):  
Jefferson O. Abaricia ◽  
Arth H. Shah ◽  
Manotri Chaubal ◽  
Kelly M. Hotchkiss ◽  
Rene Olivares-Navarrete
Keyword(s):  
Wnt Signaling ◽  
Surface Properties ◽  
Macrophage Polarization ◽  
Biomaterial Surface

scholarly journals Crosstalk between adipocytes and M2 macrophages compensates for osteopenic phenotype in the Lrp5-deficient mice

2020 ◽  
pp. 153537022097232
Author(s):  
Lisha Li ◽  
Xuemin Qiu ◽  
Na Zhang ◽  
Yan Sun ◽  
Yan Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wnt Signaling ◽  
Macrophage Polarization ◽  
Myeloid Cells ◽  
Osteogenic Potential ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tartrate Resistant Acid Phosphatase ◽  
M2 Macrophage ◽  
M2 Macrophages ◽  
Alizarin Red

A loss-of-function mutation in the Lrp5 gene in mice leads to a low bone mass disorder due to the inhibition of the canonical Wnt signaling pathway; however, the role of bone marrow microenvironment in mice with this mutation remains unclear. In this study, we evaluated proliferation and osteogenic potential of mouse osteoblasts using the MTT assay and Alizarin red staining. The levels of alkaline phosphatase, tartrate-resistant acid phosphatase, and adiponectin in culture supernatants were measured using the enzyme-linked immunosorbent assay. Osteoclast bone resorbing activity was evaluated by toluidine staining and the number and area of bone resorption pits were determined. We observed increased osteogenesis in osteoblasts co-cultured with the BM-derived myeloid cells compared to the osteoblasts cultured alone. Mice with global Lrp5 deletion had a relatively higher bone density compared to the mice carrying osteoblast/osteocyte-specific Lrp5 deletion. An increased frequency of M2 macrophages and reduced expression of inflammatory cytokines were detected in the myeloid cells derived from the bone marrow of mice with global Lrp5 deletion. Higher adipogenic potential and elevated levels of adiponectin in the global Lrp5 deletion mice contributed to the preferential M2 macrophage polarization. Here, we identified a novel systemic regulatory mechanism of bone formation and degradation in mice with global Lrp5 deletion. This mechanism depends on a crosstalk between the adipocytes and M2 macrophages in the bone marrow and is responsible for partly rescuing osteopenia developed as a result of decreased Wnt signaling.

scholarly journals Tailoring biomaterial surface properties to modulate host-implant interactions: implication in cardiovascular and bone therapy

2016 ◽  
Vol 4 (9) ◽  
pp. 1586-1599 ◽  
Author(s):  
Settimio Pacelli ◽  
Vijayan Manoharan ◽  
Anna Desalvo ◽  
Nikita Lomis ◽  
Kartikeya Singh Jodha ◽  
...  
Keyword(s):  
Medical Device ◽  
Surface Properties ◽  
Critical Role ◽  
Host Body ◽  
Biomaterial Surface ◽  
Post Implantation ◽  
Body Response

Host body response to a foreign medical device plays a critical role in defining its fate post implantation.

Biomaterial surface properties modulate in vitro rat calvaria osteoblasts response: Roughness and or chemistry?

2008 ◽  
Vol 28 (5-6) ◽  
pp. 990-1001 ◽  
Author(s):  
Carine Wirth ◽  
Brigitte Grosgogeat ◽  
Christelle Lagneau ◽  
Nicole Jaffrezic-Renault ◽  
Laurence Ponsonnet
Keyword(s):  
Surface Properties ◽  
Rat Calvaria ◽  
Biomaterial Surface

scholarly journals Characterization and evaluation of a femtosecond laser-induced osseointegration and an anti-inflammatory structure generated on a titanium alloy

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Yang Liu ◽  
Zhongying Rui ◽  
Wei Cheng ◽  
Licheng Song ◽  
Yunqiang Xu ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Femtosecond Laser ◽  
Surface Properties ◽  
Cellular Response ◽  
Titanium Surface ◽  
Macrophage Polarization ◽  
Ti Alloy ◽  
Bone Implant ◽  
Cell Material Interactions ◽  
Factor Secretion

Abstract Cell–material interactions during early osseointegration of the bone–implant interface are critical and involve crosstalk between osteoblasts and osteoclasts. The surface properties of titanium implants also play a critical role in cell–material interactions. In this study, femtosecond laser treatment and sandblasting were used to alter the surface morphology, roughness and wettability of a titanium alloy. Osteoblasts and osteoclasts were then cultured on the resulting titanium alloy disks. Four disk groups were tested: a polished titanium alloy (pTi) control; a hydrophilic micro-dislocation titanium alloy (sandblasted Ti (STi)); a hydrophobic nano-mastoid Ti alloy (femtosecond laser-treated Ti (FTi)); and a hydrophilic hierarchical hybrid micro-/nanostructured Ti alloy [femtosecond laser-treated and sandblasted Ti (FSTi)]. The titanium surface treated by the femtosecond laser and sandblasting showed higher biomineralization activity and lower cytotoxicity in simulated body fluid and lactate dehydrogenase assays. Compared to the control surface, the multifunctional titanium surface induced a better cellular response in terms of proliferation, differentiation, mineralization and collagen secretion. Further investigation of macrophage polarization revealed that increased anti-inflammatory factor secretion and decreased proinflammatory factor secretion occurred in the early response of macrophages. Based on the above results, the synergistic effect of the surface properties produced an excellent cellular response at the bone–implant interface, which was mainly reflected by the promotion of early ossteointegration and macrophage polarization.

scholarly journals Hydrophilic titanium surfaces reduce neutrophil inflammatory response and NETosis

2020 ◽  
Vol 8 (8) ◽  
pp. 2289-2299 ◽  
Author(s):  
Jefferson O. Abaricia ◽  
Arth H. Shah ◽  
Ryan M. Musselman ◽  
Rene Olivares-Navarrete
Keyword(s):  
Inflammatory Response ◽  
Surface Properties ◽  
Inflammatory Microenvironment ◽  
Biomaterial Surface ◽  
Titanium Surfaces ◽  
Novel Target

Neutrophils are sensitive to biomaterial surface properties, controlling activation and inflammatory microenvironment, revealing a novel target for enhancing biomaterial integration.

Effect of biomaterial surface properties on fibronectin–α5β1 integrin interaction and cellular attachment

Biomaterials ◽  
2006 ◽  
Vol 27 (9) ◽  
pp. 1907-1916 ◽  
Author(s):  
Mark H. Lee ◽  
Paul Ducheyne ◽  
Laura Lynch ◽  
David Boettiger ◽  
Russell J. Composto
Keyword(s):  
Surface Properties ◽  
Cellular Attachment ◽  
Biomaterial Surface ◽  
Α5β1 Integrin

scholarly journals Inhibition of BCL9 Modulates the Cellular Landscape of Tumor-Associated Macrophages in the Tumor Immune Microenvironment of Colorectal Cancer

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhuang Wei ◽  
Mengxuan Yang ◽  
Mei Feng ◽  
Zhongen Wu ◽  
Rina Rosin-Arbesfeld ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Wnt Signaling ◽  
B Cell ◽  
Signaling Pathway ◽  
Cell Lymphoma ◽  
Macrophage Polarization ◽  
Wnt Signaling Pathway ◽  
B Cell Lymphoma ◽  
Clinical Samples ◽  
Tumor Associated Macrophages

Tumor-associated macrophages (TAMs) are an indispensable part of the tumor microenvironment (TME), and they likely play a negative rather than positive role in cancer treatment. However, the cellular landscape and transcriptional profile regulation of TAMs in the case of tumor gene inactivation or chemical interference remains unclear. The B-cell lymphoma 9/B-cell lymphoma 9-like (BCL9/BCL9L) is a critical transcription co-factor of β-catenin. Suppression of Bcl9 inhibits tumor growth in mouse models of colorectal cancer (CRC). Here, we studied the TAMs of CRC by single-cell sequencing. Bcl9 depletion caused macrophage polarization inhibition from M0 to M2 and changed the CRC TME, which further interferes with the inflammation of M0 and M1. The transcription factor regulating these processes may be related to the Wnt signaling pathway from multiple levels. Furthermore, we also found that the cells delineated from monocyte to NK-like non-functioning cells were significantly different in the BCL9-deprived population. Combining these data, we proposed a TAM-to-NK score to evaluate the dynamic balance in TME of monocyte/TAM cells and NK-like non-functioning cells in The Cancer Genome Atlas (TCGA) clinical samples to verify the clinical significance. We demonstrated that the cell type balance and transcription differences of TAMs regulated by BCL9-driven Wnt signaling affected immune surveillance and inflammation of cancer, ultimately affecting patients’ prognosis. We thereby highlighted the potential of targeting Wnt signaling pathway through cancer immunotherapy.

scholarly journals Influence of Surface Properties on the Adhesion ofStaphylococcus epidermidisto Acrylic and Silicone

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Cláudia Sousa ◽  
Pilar Teixeira ◽  
Rosário Oliveira
Keyword(s):  
Surface Properties ◽  
Staphylococcus Epidermidis ◽  
Bacterial Adhesion ◽  
Surface Characteristics ◽  
Surface Hydrophobicity ◽  
Force Microscopy ◽  
Angle Measurements ◽  
Atomic Force ◽  
Contact Angle Measurements ◽  
Biomaterial Surface

The aim of the present study was to compare the ability of eightStaphylococcus epidermidisstrains to adhere to acrylic and silicone, two polymers normally used in medical devices manufacture. Furthermore, it was tried to correlate that with the surface properties of substrata and cells. Therefore, hydrophobicity and surface tension components were calculated through contact angle measurements. Surface roughness of substrata was also assessed by atomic force microscopy (AFM). No relationship was found between microbial surface hydrophobicity and adhesion capability. Nevertheless,Staphylococcus epidermidisIE214 showed very unique adhesion behaviour, with cells highly aggregated between them, which is a consequence of their specific surface features. All strains, determined as being hydrophilic, adhered at a higher extent to silicone than to acrylic, most likely due to its more hydrophobic character and higher roughness. This demonstrates the importance of biomaterial surface characteristics for bacterial adhesion.

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