The role of the transcription factor Rbpj in the development of dorsal root ganglia and trigeminal ganglia

The expression of the homeobox gene Uncx4.1 in the somite is restricted to the caudal half of the newly formed somite and sclerotome. Here we show that mice with a targeted mutation of the Uncx4.1 gene exhibit defects in the axial skeleton and ribs. In the absence of Uncx4.1, pedicles of the neural arches and proximal ribs are not formed. In addition, dorsal root ganglia are disorganized. Histological and marker analysis revealed that Uncx4.1 is not necessary for somite segmentation. It is required to maintain the condensation of the caudal half-sclerotome, from which the missing skeletal elements are derived. The loss of proximal ribs in Pax1/Pax9 double mutants and the data presented here argue for a role of Uncx4.1 upstream of Pax9 in the caudolateral sclerotome. Our results further indicate that Uncx4.1 may be involved in the differential cell adhesion properties of the somite.

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Role of phosphatidylinositol 3-kinase in neuronal survival and axonal outgrowth of adult mouse dorsal root ganglia explants

Journal of Neuroscience Research ◽

10.1002/jnr.10686 ◽

2003 ◽

Vol 74 (5) ◽

pp. 726-735 ◽

Cited By ~ 33

Author(s):

Anders Edström ◽

Per A.R. Ekström

Keyword(s):

Dorsal Root Ganglia ◽

Dorsal Root ◽

Adult Mouse ◽

Neuronal Survival ◽

Axonal Outgrowth ◽

Phosphatidylinositol 3 Kinase ◽

Phosphatidylinositol 3

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Role of GM-CSF in a mouse model of experimental autoimmune prostatitis

AJP Renal Physiology ◽

10.1152/ajprenal.00013.2018 ◽

2019 ◽

Vol 317 (1) ◽

pp. F23-F29 ◽

Cited By ~ 3

Author(s):

Yaxiao Liu ◽

Yan Li ◽

Qinggang Liu ◽

Zonglong Wu ◽

Jianfeng Cui ◽

...

Keyword(s):

Mouse Model ◽

Dorsal Root Ganglia ◽

Knockout Mice ◽

Dorsal Root ◽

Prostate Tissue ◽

Mrna Levels ◽

Gm Csf ◽

Mouse Prostate ◽

Experimental Autoimmune

The etiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is still unknown. Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to play an important role in the development of autoimmune and inflammatory diseases. Here, we investigated the expression and function of GM-CSF in patients with CP/CPPS and in a mouse model of experimental autoimmune prostatitis (EAP). GM-CSF mRNA levels were detected in expressed prostatic secretions samples from patients with CP/CPPS and in prostate tissue from a mouse model of EAP. The expression of GM-CSF receptor in mouse prostate and dorsal root ganglia were determined using PCR and immunohistochemistry. Behavioral testing and inflammation scoring were performed to evaluate the role of GM-CSF in disease development and symptom severity of EAP using GM-CSF knockout mice. mRNA levels of putative nociceptive and inflammatory markers were measured in the prostate after the induction of EAP. Elevated GM-CSF mRNA levels were observed in expressed prostatic secretions samples from patients with CP/CPPS compared with healthy volunteers. GM-CSF mRNA was also significantly increased in prostate tissue of the EAP mice model. The expression of GM-CSF receptors was confirmed in mouse prostate and dorsal root ganglia. GM-CSF knockout mice showed fewer Infiltrating leukocytes and pain symptoms after the induction of EAP. Deletion of GM-CSF significantly diminished EAP-induced increases of chemokine (C-C motif) ligand 2, chemokine (C-C motif) ligand 3, and nerve growth factor mRNA expression. The results indicated that GM-CSF plays a functional role in the pathogenesis of EAP. GM-CSF may function as a signaling mediator for both inflammation and pain transduction in CP/CPPS.

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