Sex-specific, postpuberty changes in mouse brain structures revealed by three-dimensional magnetic resonance microscopy

NeuroImage ◽  
2004 ◽  
Vol 22 (4) ◽  
pp. 1636-1645 ◽  
Author(s):  
Kyoko Koshibu ◽  
Pat Levitt ◽  
Eric T Ahrens
Keyword(s):  
Magnetic Resonance ◽  
Mouse Brain ◽  
Three Dimensional ◽  
Brain Structures ◽  
Magnetic Resonance Microscopy

A three-dimensional digital atlas database of the adult C57BL/6J mouse brain by magnetic resonance microscopy

Neuroscience ◽  
2005 ◽  
Vol 135 (4) ◽  
pp. 1203-1215 ◽  
Author(s):  
Y. Ma ◽  
P.R. Hof ◽  
S.C. Grant ◽  
S.J. Blackband ◽  
R. Bennett ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Mouse Brain ◽  
Three Dimensional ◽  
Magnetic Resonance Microscopy ◽  
Digital Atlas

scholarly journals Three-dimensional magnetic resonance microscopy of pulmonary solitary tumors in transgenic mice

2006 ◽  
Vol 56 (3) ◽  
pp. 698-703 ◽  
Author(s):  
Shigeto Kubo ◽  
Elena Levantini ◽  
Susumu Kobayashi ◽  
Olivier Kocher ◽  
Balazs Halmos ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Transgenic Mice ◽  
Three Dimensional ◽  
Magnetic Resonance Microscopy

scholarly journals Vascular phenotyping of brain tumors using magnetic resonance microscopy (μMRI)

2011 ◽  
Vol 31 (7) ◽  
pp. 1623-1636 ◽  
Author(s):  
Eugene Kim ◽  
Jiangyang Zhang ◽  
Karen Hong ◽  
Nicole E Benoit ◽  
Arvind P Pathak
Keyword(s):  
Brain Tumor ◽  
Magnetic Resonance ◽  
Brain Tumors ◽  
Three Dimensional ◽  
Region Of Interest ◽  
Tumor Model ◽  
Magnetic Resonance Microscopy ◽  
Vascular Phenotype ◽  
Brain Tumor Model ◽  
Novel Method

Abnormal vascular phenotypes have been implicated in neuropathologies ranging from Alzheimer's disease to brain tumors. The development of transgenic mouse models of such diseases has created a crucial need for characterizing the murine neurovasculature. Although histologic techniques are excellent for imaging the microvasculature at submicron resolutions, they offer only limited coverage. It is also challenging to reconstruct the three-dimensional (3D) vasculature and other structures, such as white matter tracts, after tissue sectioning. Here, we describe a novel method for 3D whole-brain mapping of the murine vasculature using magnetic resonance microscopy (μMRI), and its application to a preclinical brain tumor model. The 3D vascular architecture was characterized by six morphologic parameters: vessel length, vessel radius, microvessel density, length per unit volume, fractional blood volume, and tortuosity. Region-of-interest analysis showed significant differences in the vascular phenotype between the tumor and the contralateral brain, as well as between postinoculation day 12 and day 17 tumors. These results unequivocally show the feasibility of using μMRI to characterize the vascular phenotype of brain tumors. Finally, we show that combining these vascular data with coregistered images acquired with diffusion-weighted MRI provides a new tool for investigating the relationship between angiogenesis and concomitant changes in the brain tumor microenvironment.

scholarly journals In vivo continuous three-dimensional magnetic resonance microscopy: a study of metamorphosis in Carniolan worker honey bees (Apis mellifera carnica)

2020 ◽  
Vol 223 (21) ◽  
pp. jeb225250
Author(s):  
Aleš Mohorič ◽  
Janko Božič ◽  
Polona Mrak ◽  
Kaja Tušar ◽  
Chenyun Lin ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Magnetic Resonance ◽  
Time Course ◽  
Three Dimensional ◽  
The Body ◽  
Magnetic Resonance Microscopy ◽  
Tracheal System ◽  
Apis Mellifera Carnica ◽  
Occurrence Matrix

ABSTRACTThree-dimensional (3D) magnetic resonance microscopy (MRM) is a modality of magnetic resonance imaging (MRI) optimized for the best resolution. Metamorphosis of the Carniolan worker honey bee (Apis mellifera carnica) was studied in vivo under controlled temperature and humidity conditions from sealed larvae until the emergence of an adult. The 3D images were analyzed by volume rendering and segmentation, enabling the analysis of the body, tracheal system and gastrointestinal tract through the time course of volume changes. Fat content sensitivity enabled the analysis of flight muscles transformation during the metamorphosis by the signal histogram and gray level co-occurrence matrix (GLCM). Although the transformation during metamorphosis is well known, MRM enables an alternative insight to this process, i.e. 3D in vivo, which has relatively high spatial and temporal resolutions. The developed methodology can easily be adapted for studying the metamorphosis of other insects or any other incremental biological process on a similar spatial and temporal scale.

scholarly journals Three-dimensional visualization of protein expression in mouse brain structures using imaging mass spectrometry

2005 ◽  
Vol 16 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
Anna C. Crecelius ◽  
D. Shannon Cornett ◽  
Richard M. Caprioli ◽  
Betsy Williams ◽  
Benoit M. Dawant ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Expression ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Three Dimensional ◽  
Brain Structures

scholarly journals Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging

2012 ◽  
Vol 44 (15) ◽  
pp. 778-785 ◽  
Author(s):  
Jacqueline A. Gleave ◽  
Michael D. Wong ◽  
Jun Dazai ◽  
Maliha Altaf ◽  
R. Mark Henkelman ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Mouse Brain ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Brain Structures ◽  
Small Scale ◽  
Specimen Preparation ◽  
Normal Brain ◽  
Autofluorescence Imaging ◽  
The Brain

The structural organization of the brain is important for normal brain function and is critical to understand in order to evaluate changes that occur during disease processes. Three-dimensional (3D) imaging of the mouse brain is necessary to appreciate the spatial context of structures within the brain. In addition, the small scale of many brain structures necessitates resolution at the ∼10 μm scale. 3D optical imaging techniques, such as optical projection tomography (OPT), have the ability to image intact large specimens (1 cm3) with ∼5 μm resolution. In this work we assessed the potential of autofluorescence optical imaging methods, and specifically OPT, for phenotyping the mouse brain. We found that both specimen size and fixation methods affected the quality of the OPT image. Based on these findings we developed a specimen preparation method to improve the images. Using this method we assessed the potential of optical imaging for phenotyping. Phenotypic differences between wild-type male and female mice were quantified using computer-automated methods. We found that optical imaging of the endogenous autofluorescence in the mouse brain allows for 3D characterization of neuroanatomy and detailed analysis of brain phenotypes. This will be a powerful tool for understanding mouse models of disease and development and is a technology that fits easily within the workflow of biology and neuroscience labs.

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