Measurement of fibre–fibre contact in three-dimensional images of fibrous materials obtained from X-ray synchrotron microtomography

Author(s):  
F. Malmberg ◽  
J. Lindblad ◽  
C. Östlund ◽  
K.M. Almgren ◽  
E.K. Gamstedt

Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.



2009 ◽  
Vol 44 (8) ◽  
pp. 1203-1224 ◽  
Author(s):  
A. Tsuchiyama ◽  
T. Nakamura ◽  
T. Okazaki ◽  
K. Uesugi ◽  
T. Nakano ◽  
...  


2005 ◽  
Vol 49 (1) ◽  
pp. 84-92
Author(s):  
Hiroko Misawa ◽  
Soichiro Tsuchiya ◽  
Norichika Sasaki ◽  
Takahiro Hagihara ◽  
Youhei Fujiseki ◽  
...  


Parasitology ◽  
2017 ◽  
Vol 145 (7) ◽  
pp. 848-854 ◽  
Author(s):  
James D. B. O'Sullivan ◽  
Julia Behnsen ◽  
Tobias Starborg ◽  
Andrew S. MacDonald ◽  
Alexander T. Phythian-Adams ◽  
...  

AbstractX-ray micro-computed tomography (μCT) is a technique which can obtain three-dimensional images of a sample, including its internal structure, without the need for destructive sectioning. Here, we review the capability of the technique and examine its potential to provide novel insights into the lifestyles of parasites embedded within host tissue. The current capabilities and limitations of the technology in producing contrast in soft tissues are discussed, as well as the potential solutions for parasitologists looking to apply this technique. We present example images of the mouse whipworm Trichuris muris and discuss the application of μCT to provide unique insights into parasite behaviour and pathology, which are inaccessible to other imaging modalities.



Author(s):  
Victoria Krasnoshlyk ◽  
Sabine Rolland du Roscoat ◽  
Pierre J. J. Dumont ◽  
Per Isaksson ◽  
Edward Ando ◽  
...  

The structural changes that are induced by the initiation and the propagation of a crack in a low-density paper (LDP) were studied using single edge-notched fracture tests that were imaged under an optical microscope or in laboratory or synchrotron X-ray microtomographs. The two-dimensional optical images were used to analyse the links between the mesoscale structural variations of LDP and the crack path. Medium-resolution X-ray three-dimensional images were used to analyse the variations in the thickness and local porosity of samples as well as their displacement field that were induced by the LDP fracture. High-resolution three-dimensional images showed that these mesostructural variations were accompanied by complex fibre and bond deformation mechanisms that were, for the first time, in situ imaged. These mechanisms occurred in the fracture process zone that developed ahead of the crack tip before the crack path became distinct and visible. They were at the origin of the aforementioned thickness variations that developed more particularly along the crack path. They eventually led to fibre–fibre bond detachment phenomena and crack propagation through the fibrous network. These results can be used to enhance the current structural and mechanical models for the prediction of the fracture behaviour of papers.



2011 ◽  
Vol 46 (7) ◽  
pp. 549-561 ◽  
Author(s):  
H Toda ◽  
E Maire ◽  
Y Aoki ◽  
M Kobayashi


Author(s):  
James A. Lake

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.



Author(s):  
S. Cusack ◽  
J.-C. Jésior

Three-dimensional reconstruction techniques using electron microscopy have been principally developed for application to 2-D arrays (i.e. monolayers) of biological molecules and symmetrical single particles (e.g. helical viruses). However many biological molecules that crystallise form multilayered microcrystals which are unsuitable for study by either the standard methods of 3-D reconstruction or, because of their size, by X-ray crystallography. The grid sectioning technique enables a number of different projections of such microcrystals to be obtained in well defined directions (e.g. parallel to crystal axes) and poses the problem of how best these projections can be used to reconstruct the packing and shape of the molecules forming the microcrystal.Given sufficient projections there may be enough information to do a crystallographic reconstruction in Fourier space. We however have considered the situation where only a limited number of projections are available, as for example in the case of catalase platelets where three orthogonal and two diagonal projections have been obtained (Fig. 1).



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