Engineering the 3D Tumour microenvironment in vitro for Drug Discovery using Self-Assembling Peptide Hydrogels

Pancreatology ◽  
2020 ◽  
Vol 20 (8) ◽  
pp. e21
Author(s):  
A. Del Rio Hernandez ◽  
D. Lachowski ◽  
A. Miller
Keyword(s):  
Drug Discovery ◽  
Tumour Microenvironment ◽  
Self Assembling ◽  
Peptide Hydrogels

scholarly journals Self-Assembling Peptide Hydrogels Modulate In Vitro Chondrogenesis of Bovine Bone Marrow Stromal Cells

2010 ◽  
Vol 16 (2) ◽  
pp. 465-477 ◽  
Author(s):  
Paul W. Kopesky ◽  
Eric J. Vanderploeg ◽  
John S. Sandy ◽  
Bodo Kurz ◽  
Alan J. Grodzinsky
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bovine Bone ◽  
Self Assembling ◽  
Peptide Hydrogels

scholarly journals Microstructural differences of fibrin and self-assembling peptide hydrogels in dental pulp stem cell behavior: the effect of chlorite-oxidized oxyamylose

2020 ◽  
Author(s):  
Mostafa EzEldeen ◽  
Burak Toprakhisar ◽  
Denise Murgia ◽  
Nick Smisdom ◽  
Olivier Deschaume ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Microstructural Stability ◽  
Microscopy Imaging ◽  
Force Microscopy ◽  
Regenerative Endodontics ◽  
Self Assembling ◽  
Atomic Force ◽  
Peptide Hydrogels

Abstract Tailored hydrogels mimicking the native extracellular environment could aid in overcoming the high variability in regenerative endodontics outcomes. This study aimed to evaluate the effect of the chemokine-binding and antimicrobial polymer, chlorite-oxidized oxyamylose (COAM), on the microstructural properties of fibrin and self-assembling peptide (SAP) hydrogels. Further, to assess the influence of the microstructural differences between the hydrogels on the in vitro behavior of dental pulp stem cells (DPSCs).Structural and mechanical characterization of the hydrogels with and without COAM was performed by atomic force microscopy and scanning electron microscopy to characterize their microstructure (roughness and fiber length, diameter, straightness and alignment) and by nanoindentation to measure their stiffness (elastic modulus). DPSCs were encapsulated in hydrogels with and without COAM. Cell viability and circularity was determined using confocal microscopy imaging, and proliferation was determined using DNA quantification. Inclusion of COAM did not alter the microstructure of the fibrin hydrogels at the fiber level, while affecting the SAP hydrogel microstructure (homogeneity) leading to fiber aggregation. The stiffness of the SAP hydrogels was 7-fold higher than the fibrin hydrogels. The viability and attachment of DPSCs and DNA content was significantly higher in fibrin hydrogels than in SAP hydrogels. The microstructural stability after COAM inclusion and the favorable DPSCs’ response observed in fibrin hydrogels suggest this system as a promising carrier for COAM and for application in endodontic regeneration.

scholarly journals Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mostafa EzEldeen ◽  
Burak Toprakhisar ◽  
Denise Murgia ◽  
Nick Smisdom ◽  
Olivier Deschaume ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Microstructural Stability ◽  
Force Microscopy ◽  
Regenerative Endodontics ◽  
Self Assembling ◽  
Atomic Force ◽  
Human Dental Pulp ◽  
Peptide Hydrogels

AbstractTailored hydrogels mimicking the native extracellular environment could help overcome the high variability in outcomes within regenerative endodontics. This study aimed to evaluate the effect of the chemokine-binding and antimicrobial polymer, chlorite-oxidized oxyamylose (COAM), on the microstructural properties of fibrin and self-assembling peptide (SAP) hydrogels. A further goal was to assess the influence of the microstructural differences between the hydrogels on the in vitro behavior of human dental pulp stem cells (hDPSCs). Structural and mechanical characterization of the hydrogels with and without COAM was performed by atomic force microscopy and scanning electron microscopy to characterize their microstructure (roughness and fiber length, diameter, straightness, and alignment) and by nanoindentation to measure their stiffness (elastic modulus). Then, hDPSCs were encapsulated in hydrogels with and without COAM. Cell viability and circularity were determined using confocal microscopy, and proliferation was determined using DNA quantification. Inclusion of COAM did not alter the microstructure of the fibrin hydrogels at the fiber level while affecting the SAP hydrogel microstructure (homogeneity), leading to fiber aggregation. The stiffness of the SAP hydrogels was sevenfold higher than the fibrin hydrogels. The viability and attachment of hDPSCs were significantly higher in fibrin hydrogels than in SAP hydrogels. The DNA content was significantly affected by the hydrogel type and the presence of COAM. The microstructural stability after COAM inclusion and the favorable hDPSCs' response observed in fibrin hydrogels suggest this system as a promising carrier for COAM and application in endodontic regeneration.

scholarly journals Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro

2018 ◽  
Vol Volume 13 ◽  
pp. 6717-6733 ◽  
Author(s):  
Franziska Koch ◽  
Anne Wolff ◽  
Stephanie Mathes ◽  
Uwe Pieles ◽  
Sina Saxer ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Periodontal Tissue ◽  
Self Assembling ◽  
Tissue Cells ◽  
Peptide Hydrogels

scholarly journals Self-Assembling Peptide Hydrogels - PeptiGels® as a Platform for Hepatic Organoid Culture

2021 ◽  
Author(s):  
Adedamola Olayanju ◽  
Aline F Miller ◽  
Tahera Ansari ◽  
Christopher E. Goldring
Keyword(s):  
Ex Vivo ◽  
3D Culture ◽  
Molecular Techniques ◽  
3 Dimensional ◽  
Culture Systems ◽  
Self Assembling ◽  
Technological Platform ◽  
Peptide Hydrogels

AbstractA major challenge in advancing preclinical studies is the lack of robust in vitro culture systems that fully recapitulate the in vivo scenario together with limited clinical translational to humans. Organoids, as 3-dimensional (3D) self-replicating structures are increasingly being shown as powerful models for ex vivo experimentation in the field of regenerative medicine and drug discovery. Organoid formation requires the use of extracellular matrix (ECM) components to provide a 3D platform. However, the most commonly used ECM, essential for maintaining organoid growth is Matrigel and is derived from a tumorigenic source which limits its translational ability. PeptiGels® which are self-assembling peptide hydrogels present as alternatives to traditional ECM for use in 3D culture systems. Synthetic PeptiGels® are non-toxic, biocompatible, biodegradable and can be tuneable to simulate different tissue microenvironments. In this study, we validated the use of different types of PeptiGels® for porcine hepatic organoid growth. Hepatic organoids were assessed morphologically and using molecular techniques to determine the optimum PeptiGel® formulation. The outcome clearly demonstrated the ability of PeptiGel® to support organoid growth and offer themselves as a technological platform for 3D cultured physiologically and clinically relevant data.

Torus Circumference and Thickness Parameters From a Linear DNA Size Series Suggest How Toruses Self-Assemble

1985 ◽  
Vol 43 ◽  
pp. 522-523
Author(s):  
George C. Ruben ◽  
Kenneth A. Marx
Keyword(s):  
Tertiary Structure ◽  
Dna Complexes ◽  
Tertiary Structures ◽  
Self Assembling ◽  
Double Stranded Dna ◽  
Calf Thymus ◽  
Dna Organization ◽  
Condensed Dna ◽  
Dna Size

Certain double stranded DNA bacteriophage and viruses are thought to have their DNA organized into large torus shaped structures. Morphologically, these poorly understood biological DNA tertiary structures resemble spermidine-condensed DNA complexes formed in vitro in the total absence of other macromolecules normally synthesized by the pathogens for the purpose of their own DNA packaging. Therefore, we have studied the tertiary structure of these self-assembling torus shaped spermidine- DNA complexes in a series of reports. Using freeze-etch, low Pt-C metal (10-15Å) replicas, we have visualized the microscopic DNA organization of both calf Thymus( CT) and linear 0X-174 RFII DNA toruses. In these structures DNA is circumferentially wound, continuously, around the torus into a semi-crystalline, hexagonal packed array of parallel DNA helix sections.

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