Open study of photodynamic therapy for skin ulcers infected with MRSA and Pseudomonas aeruginosa

2021 ◽  
pp. 102484
Author(s):  
Miyu Shiratori ◽  
Toshiyuki Ozawa ◽  
Nobuhisa Ito ◽  
Kunio Awazu ◽  
Daisuke Tsuruta
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
Open Study ◽  
Skin Ulcers

scholarly journals An Eschar-like souvenir from a journey to Colombia: Ecthyma gangrenosum as a differential diagnosis of tropical diseases in immunocompromised patients – a case report

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Gabriela M. Wiedemann ◽  
Jochen Schneider ◽  
Mareike Verbeek ◽  
Björn Konukiewitz ◽  
Christoph D. Spinner ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Differential Diagnosis ◽  
Antibiotic Treatment ◽  
Partial Remission ◽  
Combined Treatment ◽  
Lymphocytic Leukemia ◽  
Entire Body ◽  
Tropical Diseases ◽  
Ecthyma Gangrenosum ◽  
Skin Ulcers

Abstract Background Ecthyma gangrenosum (EG) is a cutaneous infectious disease characterized by eschar-like skin ulcers typically caused by Pseudomonas aeruginosa. Here, we report a case of relapsing EG in a patient who had returned from a trip to Colombia, thus establishing EG as an important differential diagnosis of tropical diseases, and demonstrating that even long-term antibiotic treatment can result in only partial remission of EG. Case presentation A 77-year-old man with underlying chronic lymphocytic leukemia (CLL) on ibrutinib treatment was admitted because of a superinfected mosquito bite on the left ear and multiple partially necrotic skin lesions disseminated all over the entire body five days after returning from a trip to Colombia. The initial clinical suspicion of a tropical disease (leishmaniosis, systemic mycosis, or others) could not be confirmed. During the diagnostic workup, microbiological cultures of the skin biopsies and bronchoalveolar lavage revealed Pseudomonas aeruginosa, leading to a diagnosis of EG. Initial antibiotic treatment resulted in partial remission. However, the patient had to be re-admitted due to a relapse 3–4 weeks after the first episode. Finally, the patient was successfully treated with a combined approach consisting of antibiotics, recurrent surgical incisions, and administration of immunoglobulins. Conclusions In conclusion, EG should be considered as a differential diagnosis in immunosuppressed patients presenting with eschar-like skin ulcers. A combined treatment approach seems to be the best choice to achieve clinical cure and avoid relapse.

scholarly journals Antimicrobial effect of methylene blue formulations with oxygen carrier at different pHs: preliminary study

2019 ◽  
Vol 22 (1) ◽  
pp. Process
Author(s):  
Jessica Klöckner Knorst ◽  
Gabriela Scarton Barriquello ◽  
Marcos Antônio Villetti ◽  
Roberto Christ Vianna Santos ◽  
Karla Zanini Kantorski
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
Oxygen Carrier ◽  
Statistical Significance ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Preliminary Study ◽  
Post Hoc ◽  
Multispecies Biofilms

Objective: Evaluate methylene blue (MB) formulations containing oxygen carrier at different pHs in antimicrobial photodynamic therapy (aPDT). Material and Methods: Biofilms of Pseudomonas aeruginosa PA01 formed over acrylics specimens during five days were treated with aPDT using different formulations: MB/pH 7.4; MB/pH 5.6; MB/carrier pH 7.4; MB/carrier pH 5.6. Biofilms not exposed to treatment were used as a control. Blind examiner for the experimental groups performed the counting of colonies per ml suspension (CFU/ml). Two-way ANOVA was used to determine the effect of factors solvent (carrier vs water) and pH (7.4 vs 5.6). One-way ANOVA and post-hoc Tukey’s test was used to evaluate differences among the five groups (control; MB/carrier pH 7.4; MB pH 7.4; MB/carrier pH 5.6; MB pH 5.6). The Statistics 8.0 software was used (P<0.05). Results: All of photodynamic therapy groups showed significant reduction in P. aeruginosa compared to the control group. The solvent factor was not significant (P=0.18), while the pH factor presented statistical significance (P=0.01). When the carrier was used, MB formulation at pH 7.4 presented a statistically greater reduction of P. aeruginosa than the formulation with pH 5.6. Conclusion: The PDT using methylene blue formulations with oxygen carrier demonstrated potential for the treatment of localized infections by P. aeruginosa. MB formulations with oxygen carrier and pH 7.4 resulted in higher antimicrobial effect and should be considered for future studies with multispecies biofilms.  KeywordsAntimicrobial photodynamic therapy; biofilm; laser; Pseudomonas aeruginosa.

Photodynamic therapy using methylene blue, combined or not with gentamicin, against Staphylococcus aureus and Pseudomonas aeruginosa

2020 ◽  
Vol 31 ◽  
pp. 101810
Author(s):  
Vanesa Pérez-Laguna ◽  
Isabel García-Luque ◽  
Sofía Ballesta ◽  
Luna Pérez-Artiaga ◽  
Verónica Lampaya-Pérez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methylene Blue ◽  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy

scholarly journals 61* Photodynamic therapy against antibiotic resistant Pseudomonas aeruginosa isolates from CF patients

2007 ◽  
Vol 6 ◽  
pp. S15
Author(s):  
O.Y. Torun ◽  
H. Uludag ◽  
S. Pekcan ◽  
B. Sener ◽  
D. Dogru ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
Antibiotic Resistant

scholarly journals NITRIC OXIDE METABOLISM FEATURES UNDER CONDITIONS OF EXPERIMENTAL INFECTED RADIATION-INDUCED SKIN INJURIES DEVELOPMENT AND THEIR TREATMENT WITH PHOTODYNAMIC THERAPY

2020 ◽  
Vol 73 (8) ◽  
pp. 1655-1658
Author(s):  
Mykola V. Krasnoselskyi ◽  
Elena S. Pushkar ◽  
Larisa I. Simonova-Pushkar ◽  
Mykhailo S. Myroshnychenko
Keyword(s):  
Nitric Oxide ◽  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Blood Serum ◽  
Skin Ulcer ◽  
Skin Injuries ◽  
Skin Ulcers ◽  
Nitric Oxide Metabolism ◽  
Radiation Induced ◽  
Nitric Oxide Metabolites

The aim: To follow-up nitric oxide content values in rat serum at the development of Staphylococcus aureus infected radiation skin injuries and their photodynamic therapy. Materials and methods: Eighty WAG male rats were studied in an experiment. Four groups were identified for evaluation. Group 1 included unaffected intact rats (n=20). Group 2 involved rats (n=20) with a modeled radiation-induced ulcer of the skin. The rats (n=20) with a modeled radiation-induced skin ulcer followed by infecting with Staphylococcus aureus were referred to group 3. Group 4 included rats (n=20) with Staphylococcus aureus infected radiation skin ulcer exposed to photodynamic therapy. Rats of groups 1-4 were sampled for biochemical blood examination on days 7, 14, 21, 30 and 45. Total nitric oxide metabolites (nitrites and nitrates) were measured according to V.A. Metelskaya et al. method. Results: Infectious agent (Staphylococcus aureus) present in skin ulcer impairs nitric oxide metabolism in rat blood serum that manifested in decreased total nitric oxide metabolites content on day 7, followed by its increase within days 14 to 45. While photodynamic therapy exposed on the Staphylococcus aureus infected radiation skin ulcer, total nitric oxide metabolites in blood serum had increased by day 7, but days 14 to 45 level was compliant with physiological norm. Conclusions: Infecting radiation skin ulcers with Staphylococcus aureus causes impaired nitric oxide metabolism, while photodynamic therapy helps to normalize the metabolism of the above-mentioned chemical compound that can improve healing of radiation skin ulcers.

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