scholarly journals Antimicrobial effect of methylene blue formulations with oxygen carrier at different pHs: preliminary study

2019 ◽  
Vol 22 (1) ◽  
pp. Process
Author(s):  
Jessica Klöckner Knorst ◽  
Gabriela Scarton Barriquello ◽  
Marcos Antônio Villetti ◽  
Roberto Christ Vianna Santos ◽  
Karla Zanini Kantorski
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
Oxygen Carrier ◽  
Statistical Significance ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Preliminary Study ◽  
Post Hoc ◽  
Multispecies Biofilms

Objective: Evaluate methylene blue (MB) formulations containing oxygen carrier at different pHs in antimicrobial photodynamic therapy (aPDT). Material and Methods: Biofilms of Pseudomonas aeruginosa PA01 formed over acrylics specimens during five days were treated with aPDT using different formulations: MB/pH 7.4; MB/pH 5.6; MB/carrier pH 7.4; MB/carrier pH 5.6. Biofilms not exposed to treatment were used as a control. Blind examiner for the experimental groups performed the counting of colonies per ml suspension (CFU/ml). Two-way ANOVA was used to determine the effect of factors solvent (carrier vs water) and pH (7.4 vs 5.6). One-way ANOVA and post-hoc Tukey’s test was used to evaluate differences among the five groups (control; MB/carrier pH 7.4; MB pH 7.4; MB/carrier pH 5.6; MB pH 5.6). The Statistics 8.0 software was used (P<0.05). Results: All of photodynamic therapy groups showed significant reduction in P. aeruginosa compared to the control group. The solvent factor was not significant (P=0.18), while the pH factor presented statistical significance (P=0.01). When the carrier was used, MB formulation at pH 7.4 presented a statistically greater reduction of P. aeruginosa than the formulation with pH 5.6. Conclusion: The PDT using methylene blue formulations with oxygen carrier demonstrated potential for the treatment of localized infections by P. aeruginosa. MB formulations with oxygen carrier and pH 7.4 resulted in higher antimicrobial effect and should be considered for future studies with multispecies biofilms.  KeywordsAntimicrobial photodynamic therapy; biofilm; laser; Pseudomonas aeruginosa.

Photodynamic therapy using methylene blue, combined or not with gentamicin, against Staphylococcus aureus and Pseudomonas aeruginosa

2020 ◽  
Vol 31 ◽  
pp. 101810
Author(s):  
Vanesa Pérez-Laguna ◽  
Isabel García-Luque ◽  
Sofía Ballesta ◽  
Luna Pérez-Artiaga ◽  
Verónica Lampaya-Pérez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methylene Blue ◽  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy

scholarly journals Comparative Effects of Photodynamic Therapy mediated by Curcumin on Standard and Clinical Isolate of Streptococcus mutans

2015 ◽  
Vol 16 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Marco Aurelio Paschoal ◽  
Caroline C Tonon ◽  
Marilia Correia ◽  
Denise MP Spolidório ◽  
Vanderlei S Bagnato ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
Clinical Isolate ◽  
Light Source ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Lower Sensitivity ◽  
Bacterial Reduction ◽  
Blue Wavelength ◽  
Suspension Model

ABSTRACT Aim The aim of this study was investigate the effect of photodynamic therapy (PDT) using curcumin (C) as a photosensitizing agent irradiated with an LED (L) in the blue wavelength as a light source on a standard and clinical isolate of Streptococcus mutans (S. mutans) in a planktonic suspension model. Materials and methods Suspensions of both strains were divided into 4 groups as follows: absence of C and L (control group: C–L–), with C and without L (C group: C+L–), absence of C with L (L group: C–L+) and presence of C and L (PDT group: C+L+). Three different concentrations of curcumin (0.75 mg/ml, 1.5 mg/ml and 3 mg/ml) and three light fluences of studied light source (24, 48 and 72 J cm–2) were tested. Aliquots of each studied group was plated in BHI agar and submitted to colony forming units counting (CFU/ml) and the data transformed into logarithmical scale. Results A high photoinactivation rate of more than 70% was verified to standard S. mutans strain submitted to PDT whereas the clinical isolate showed a lower sensitivity to all the associations of curcumin and LED. A slight bacterial reduction was verified to C+L– and C–L+, demonstrating no toxic effects to the isolated application of light and photosensitizer to both S. mutans strains tested. Conclusion Photodynamic therapy using a combination of curcumin and blue LED presented a substantial antimicrobial effect on S. mutans standard strain in a planktonic suspension model with a less pronounced effect on its clinical isolate counterparts due to resistance to this alternative approach. Clinical significance Alternative antimicrobial approaches, as photodynamic therapy, should be encouraged due to optimal results against cariogenic bacteria aiming to prevent or treat dental caries. How to cite this article Tonon CC, Paschoal MA, Correia M, Spolidório DMP, Bagnato VS, Giusti JSM, Santos-Pinto L. Comparative Effects of Photodynamic Therapy mediated by Curcumin on Standard and Clinical Isolate of Streptococcus mutans. J Contemp Dent Pract 2015;16(1):1-6.

scholarly journals Cerebrospinal Fluid Biomarkers in Spinocerebellar Ataxia: A Pilot Study

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Ashley M. Brouillette ◽  
Gülin Öz ◽  
Christopher M. Gomez
Keyword(s):  
Cerebrospinal Fluid ◽  
Statistical Significance ◽  
Control Group ◽  
Csf Biomarkers ◽  
Spinocerebellar Ataxias ◽  
Disease Subtype ◽  
Cerebrospinal Fluid Biomarkers ◽  
Sporadic Disease ◽  
Cerebellar Type ◽  
Post Hoc

Neurodegenerative diseases, including the spinocerebellar ataxias (SCA), would benefit from the identification of reliable biomarkers that could serve as disease subtype-specific and stage-specific indicators for the development and monitoring of treatments. We analyzed the cerebrospinal fluid (CSF) level of tau,α-synuclein, DJ-1, and glial fibrillary acidic protein (GFAP), proteins previously associated with neurodegenerative processes, in patients with the autosomal dominant SCA1, SCA2, and SCA6, and the sporadic disease multiple system atrophy, cerebellar type (MSA-C), compared with age-matched controls. We estimated disease severity using the Scale for the Assessment and Rating of Ataxia (SARA). Most proteins measured trended higher in disease versus control group yet did not reach statistical significance. We found the levels of tau in both SCA2 and MSA-C patients were significantly higher than control. We found thatα-synuclein levels were lower with higher SARA scores in SCA1 and tau levels were higher with greater SARA in MSA-C, although this final correlation did not reach statistical significance after post hoc correction. Additional studies with larger sample sizes are needed to improve the power of these studies and validate the use of CSF biomarkers in SCA and MSA-C.

scholarly journals ANTIMICROBIAL EFFECT OF A MEANS FOR ENZYME EXCAVATION (BRIX 3000) AND PHOTODYNAMIC THERAPY IN CARIOUS LESIONS OF PRIMARY TEETH – IN VITRO EXPERIMENT

2021 ◽  
Vol 27 (4) ◽  
pp. 4048-4051
Author(s):  
Zornitsa Lazarova ◽  
◽  
Maya Rashkova ◽  
Raina Gergova ◽  
Virna-Maria Tsitou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Photodynamic Therapy ◽  
Primary Teeth ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Carious Lesions ◽  
Lactobacillus Spp

The selective removal of the caries dentin via enzyme methods for excavation appears to be an alternative to the conventional treatment of carious lesions in childhood. Photodynamic therapy is an effective alternative for the reduction of cariesogenic microorganisms. Brix 3000 is an enzyme-based material for excavation of carious dentin. Aim: To study the antimicrobial effect of means for enzyme excavation (Brix 3000) and photodynamic therapy with FotoSan 630 Intro Kit to the two main cariesogenic microorganisms – S. mutans and L. acidophilus, in vitro experiment; Materials and Methods: Eighty plates were prepared: group 1- 20 plates only with Brix 3000; group2 – 20 plates only with FotoSan; group 3 – 20 plates with a combination of Brix 3000 and FotoSan; group 4 – 20 plates without an active agent. In the agar of each plate, three 7 mm wells in diameter were made, where the Brix 3000 gel was placed, as well as discs soaked with the dye and irradiated with FotoSan and a combination of them. After 24 hours, the zone of inhibition was measured. Results: Compared to the control group, Brix 3000 and FotoSan have a defined antimicrobial activity against S. mutans and Lactobacillus spp. By combining the two materials, their antimicrobial activity significantly increases. S. mutans shows greater resistance compared to Lactobacillus spp. Conclusion: A combination of enzyme-based excavation and photodynamic therapy could be used successfully in the treatment of carious lesions in primary teeth.

scholarly journals EXPERIMENTAL STUDY OF THE NEUROTOXIC EFFECTS OF PHOTODYNAMIC THERAPY ON THE SPINAL CORD

2019 ◽  
Vol 18 (3) ◽  
pp. 176-180
Author(s):  
HERTON RODRIGO TAVARES COSTA ◽  
ELAINE APARECIDA DEL BEL BELLUZ GUIMARÃES ◽  
ANTÔNIO CLAUDIO TEDESCO ◽  
FERNANDO LUCAS PRIMO ◽  
CÉLIA APARECIDA DA SILVA ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Dura Mater ◽  
Aluminum Chloride ◽  
Control Group ◽  
Functional Evaluation ◽  
Neurotoxic Effects ◽  
Pathological Evaluation ◽  
Male Wistar Rats

ABSTRACT Objective To evaluate the effects of photodynamic therapy (PDT) on the dura mater using the photosensitizers aluminum chloride phthalocyanine and methylene blue in in vivo assays. Methods Fifty-six male Wistar rats were divided into two groups; one submitted to PDT and the other submitted to the photosensitizers without their photoactivation (control). The photosensitizers were applied to the dura mater after laminectomy at the T10 level. The methods used for assessment were the Basso, Beattie and Bresnahan (BBB) functional evaluation scale and study of the dura mater by light microscopy. Results No changes in motor activity were observed in the animals submitted to PDT compared to control. Histological and pathological evaluation did not show any differences between the group exposed to activated photosensitizers and the control group with regard to the inflammatory process and tissue necrosis. Conclusion The joint use of PDT with the photosensitizing pharmaceuticals aluminum chloride phthalocyanine and methylene blue did not induce any clinical neurotoxic effects or histological changes in the dura mater of the animals studied. Level de evidence V; Expert Opnion.

scholarly journals Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

2020 ◽  
Vol 47 (1) ◽  
pp. 70-77
Author(s):  
Choa Park ◽  
Howon Park ◽  
Juhyun Lee ◽  
Hyunwoo Seo ◽  
Siyoung Lee
Keyword(s):  
Photodynamic Therapy ◽  
Laser Scanning ◽  
Light Emitting Diode ◽  
Antimicrobial Effect ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Control Group ◽  
Antimicrobial Photodynamic Therapy ◽  
Group Dynamic ◽  
Light Emitting

The present study is aimed to assess the effect of antimicrobial photodynamic therapy (aPDT) on <i>Streptococcus mutans</i> biofilm through teeth whitening light emitting diode (LED).<br/>Planktonic and dynamic biofilm state cultures of <i>S. mutans</i> were used. Erythrosine 20 μM/L was used as the photosensitizer. Irradiation was performed by exposing cultures to clinic and homecare whitening LEDs for 15 minutes. The viability was measured through Colony Forming Unit counts and confocal laser scanning microscopy.<br/>aPDT using whitening LEDs and erythrosine significantly decreased the CFU count of <i>S. mutans</i> compared to that in the control group. Dynamic biofilm group showed more resistant features to aPDT compared with planktonic state. Clinic and homecare whitening LED device showed similar antimicrobial effect.<br/>The whitening LED, which could irradiate the entire oral arch, showed a significant photodynamic effect on cariogenic <i>S. mutans</i> biofilm. aPDT mediated by erythrosine and LEDs used for teeth whitening exhibited promising antimicrobial activity.

scholarly journals Assessment of Photodynamic Therapy and Nanoparticles Effects on Caries Models

2018 ◽  
Vol 6 (7) ◽  
pp. 1289-1295 ◽  
Author(s):  
Ali Saafan ◽  
Mohamed H. Zaazou ◽  
Marwa K. Sallam ◽  
Osama Mosallam ◽  
Heba A. El Danaf
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Diode Laser ◽  
Streptococcus Mutans ◽  
Treated Group ◽  
Nano Particles ◽  
Control Group ◽  
P Value ◽  
Ag Nps ◽  
Colony Forming Units

AIM: To assess the antibacterial competence of 650 nm diode laser, Methylene Blue (MB) and Silver Nano-Particles (Ag NPs) on Streptococcus mutans in biofilm-induced caries models.MATERIAL AND METHODS: One hundred eighty specimens were prepared and equally divided into 6 groups. One group was untreated (control), and the others were subjected to either MB, laser, Ag NPs, the combination of MB and Laser or MB, laser and Ag NPs.RESULTS: Comparison of the log10 mean Colony Forming Units per millilitre (CFU/ml) values of each of the treated 5 groups and the control group was found statistically significant (P-value < 0.05).The combination of MB, laser and Ag NPs recorded the greatest reduction (95.28%). MB alone represented the least capable (74.09%). The efficiency differences among the Ag NPs treated group; the Laser treated group and the combined MB/Laser treated group were found statistically insignificant.CONCLUSION: The combination of MB, 650 nm diode laser and Ag NPs may be among the highly effective modern antimicrobial therapeutics in dentistry.

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