Optimization of purification conditions for papain in a polyethylene glycol-phosphate aqueous two-phase system using quaternary ammonium ionic liquids as adjuvants by BBD-RSM

2019 ◽  
Vol 156 ◽  
pp. 8-16 ◽  
Author(s):  
Lei Yu ◽  
Haide Zhang ◽  
Lvqing Yang ◽  
Kangyong Tian
Keyword(s):  
Ionic Liquids ◽  
Polyethylene Glycol ◽  
Quaternary Ammonium ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Ammonium Ionic Liquids

Partition of proteins with extraction in aqueous two-phase system by hydroxyl ammonium-based ionic liquid

2014 ◽  
Vol 6 (12) ◽  
pp. 4067-4076 ◽  
Author(s):  
Jing Chen ◽  
Yuzhi Wang ◽  
Qun Zeng ◽  
Xueqin Ding ◽  
Yanhua Huang
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Ammonium Ionic Liquids

A series of hydroxyl ammonium ionic liquids have been designed and synthesized and used for the extraction of proteins.

Polyethylene glycol-modified avidin: A novel agent for the selective extraction of biotinylated immune-complex in an aqueous two-phase system

1996 ◽  
Vol 7 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Hiroyuki Nishimura ◽  
Nobuhiro Munakata ◽  
Konosuke Hayashi ◽  
Masao Hayakawa ◽  
Hideki Iwamoto ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Immune Complex ◽  
Selective Extraction ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Novel Agent

Aqueous Two-Phase Extraction of Potassium Glycyrrhizinate with Polyethylene Glycol/(NH4)2SO4

2012 ◽  
Vol 550-553 ◽  
pp. 1687-1690
Author(s):  
Chen Sun ◽  
Wen Ju Liu ◽  
Hai Ming Zhang
Keyword(s):  
Polyethylene Glycol ◽  
Mass Fraction ◽  
Ph Value ◽  
Phase System ◽  
Phase Extraction ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Result Show ◽  
A New Technique ◽  
Aqueous Two Phase Extraction

An aqueous two-phase system of polyethylene glycol (PEG)/(NH4)2SO4 with the behavior of forming aqueous two-phase and the distribution rules of potassium glycyrrhizinate in the aqueous two-phase system were studied. Effects of the molecular weight of PEG, the mass fraction of PEG and (NH4)2SO4, and pH value were investigated. The result show that when the mass fraction of PEG1000 was 50%, the mass fraction of (NH4)2SO4 was 5.7%, and pH value was 6.5, the highest extraction recovery of potassium glycyrrhizinate could reach 99.7%. This aqueous two-phase extraction may provide a new technique for separation of potassium glycyrrhizinate.

scholarly journals Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system

2017 ◽  
Vol 133 ◽  
pp. 8-14 ◽  
Author(s):  
Maria Carolina de Albuquerque Wanderley ◽  
José Manoel Wanderley Duarte Neto ◽  
Wendell Wagner Campos Albuquerque ◽  
Daniela de Araújo Viana Marques ◽  
Carolina de Albuquerque Lima ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Phase System ◽  
Purification And Characterization ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Penicillium Sp
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