Cathelicidin-NV From Nanorana ventripunctata Effectively Protects HaCaT Cells, Ameliorating Ultraviolet B-induced Skin Photoaging

Peptides ◽  
2021 ◽  
pp. 170712
Author(s):  
Guizhu Feng ◽  
Lin Wei ◽  
Helong Che ◽  
Yan Shen ◽  
Kai Mi ◽  
...  
Keyword(s):  
Ultraviolet B ◽  
Hacat Cells ◽  
Skin Photoaging

scholarly journals Protective Effect of Spirulina-Derived C-Phycocyanin against Ultraviolet B-Induced Damage in HaCaT Cells

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 273
Author(s):  
Young Ah Jang ◽  
Bo Ae Kim
Keyword(s):  
Antioxidant Defense System ◽  
Skin Aging ◽  
Ultraviolet B ◽  
Control Group ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Blue Green Algae ◽  
Radiation Group ◽  
Skin Wrinkles

Background and objectives: Reactive oxygen species (ROS) overwhelm the antioxidant defense system, induce oxidative stress, and increase matrix metalloproteinase (MMP) expression, resulting in skin aging. Thus, preventing ultraviolet B (UVB)-induced skin damage can attenuate skin aging. Spirulina (a biomass of cyanobacteria, also called blue-green algae) is comprised of prokaryotes, whereas microalgae are eukaryotes and are rich in phycocyanin, a powerful antioxidant. Materials and Methods: Here, we investigated the photoprotective effects of spirulina-derived C-phycocyanin (C-PC) against UVB radiation using keratinocytes (HaCaT cells). Results: UVB radiation increased MMP-1 and MMP-9 expression but decreased involucrin, filaggrin, and loricrin expression. C-PC showed no toxicity at concentrations of 5–80 μg/mL in terms of HaCaT cell viability. UVB-irradiated HaCaT cells had a 50.8% survival rate, which increased to 80.3% with C-PC treatment. MMP expression increased with UVB treatment, whereas MMP-1 and MMP-9 concentrations decreased with C-PC treatment. UVB reduced involucrin, filaggrin, and loricrin expression in HaCaT cells, but 80 μg/mL C-PC increased their expression by >25%. In the UVB radiation group, dichlorofluorescin diacetate fluorescence intensity in HaCaT cells increased by 81.6% compared with that in the control group, whereas ROS production was reduced by 51.2% and 55.1% upon treatment with 40 and 80 μg/mL C-PC, respectively. Conclusions: C-PC might reduce or prevent skin aging by reducing UVB irradiation-induced skin wrinkles and free radicals.

scholarly journals MicroRNA-145 attenuates IL-6-induced enhancements of sensitivity to UVB irradiation by suppressing MyD88 in HaCaT cells

2018 ◽  
Vol 32 ◽  
pp. 205873841879594 ◽  
Author(s):  
Hui Dong ◽  
Wei Jiang ◽  
Hongquan Chen ◽  
Shui Jiang ◽  
Yunshu Zang ◽  
...  
Keyword(s):  
Western Blot ◽  
Cell Viability ◽  
Lupus Erythematosus ◽  
Blot Analysis ◽  
Cell Apoptosis ◽  
Western Blot Analysis ◽  
Ultraviolet B ◽  
Hacat Cells ◽  
Related Factors ◽  
Uvb Irradiation

MicroRNAs (miRNAs/miRs) play vital roles in various immune diseases including systemic lupus erythematosus (SLE). The current study aimed to assess the role of miR-145 in interleukin-6 (IL-6)-treated HaCaT cells under ultraviolet B (UVB) irradiation and further explore the potential regulatory mechanism. HaCaT cells were pretreated with IL-6 and then exposed to UVB to assess the effect of IL-6 on sensitivity of HaCaT cells to UVB irradiation. The levels of miR-145 and MyD88 were altered by transfection and the transfected efficiency was verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR)/western blot analysis. Cell viability, percentage of apoptotic cells and expression levels of apoptosis-related factors were measured by trypan blue assay, flow cytometry assay, and western blot analysis, respectively. In addition, the levels of c-Jun N-terminal kinases (JNK) and nuclear factor-κB (NF-κB) signaling pathway-related factors were assessed by western blot analysis. IL-6 treatments significantly aggravated the reduction of cell viability and promotion of cell apoptosis caused by UVB irradiation in HaCaT cells. Interestingly, miR-145 level was augmented by UVB exposure and miR-145 mimic alleviated IL-6-induced increase of sensitivity to UVB irradiation in HaCaT cells, as dramatically increased cell viability and reduced cell apoptosis. Opposite effects were observed in miR-145 inhibitor-transfected cells. Meanwhile, MyD88 was negatively regulated by miR-145 and MyD88 mediated the regulatory effect of miR-145 on IL-6- and UVB-treated cells. In addition, miR-145 mimic inhibited the JNK and NF-κB pathways by down-regulating MyD88. In conclusion, the present study demonstrated that miR-145 alleviated IL-6-induced increase of sensitivity to UVB irradiation by down-regulating MyD88 in HaCaT cells.

scholarly journals Effect of Shenqin biochemical extract on hypoxia-inducible factor-1α expression in ultraviolet B-irradiated HaCaT cells

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
X. Chen ◽  
J. Bai ◽  
Y. Li ◽  
X.-G. Li ◽  
G.-F. Lv ◽  
...  
Keyword(s):  
Hypoxia Inducible Factor ◽  
Ultraviolet B ◽  
Hacat Cells ◽  
Hypoxia Inducible Factor 1Α

Prevention of the Ultraviolet B-Mediated Skin Photoaging by a Nuclear Factor κB Inhibitor, Parthenolide

2005 ◽  
Vol 315 (2) ◽  
pp. 624-630 ◽  
Author(s):  
Kiyotaka Tanaka ◽  
Junichi Hasegawa ◽  
Kaori Asamitsu ◽  
Takashi Okamoto
Keyword(s):  
Nuclear Factor Κb ◽  
Ultraviolet B ◽  
Nuclear Factor ◽  
Skin Photoaging

Protective effects of antioxidin-RL from Odorrana livida against ultraviolet B-irradiated skin photoaging

Peptides ◽  
2018 ◽  
Vol 101 ◽  
pp. 124-134 ◽  
Author(s):  
Di Qin ◽  
Wen-Hui Lee ◽  
Zhiqin Gao ◽  
Weifen Zhang ◽  
Meiyu Peng ◽  
...  
Keyword(s):  
Ultraviolet B ◽  
Protective Effects ◽  
Skin Photoaging

scholarly journals Increased 5-hydroxymethylcytosine and Ten-eleven Translocation Protein Expression in Ultraviolet B-irradiated HaCaT Cells

2017 ◽  
Vol 130 (5) ◽  
pp. 594-599 ◽  
Author(s):  
Dan Wang ◽  
Jin-Hua Huang ◽  
Qing-Hai Zeng ◽  
Can Gu ◽  
Shu Ding ◽  
...  
Keyword(s):  
Protein Expression ◽  
Ultraviolet B ◽  
Hacat Cells
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