Isolation and expression analysis of 18 CsbZIP genes implicated in abiotic stress responses in the tea plant (Camellia sinensis)

Abstract Background Autophagy, meaning ‘self-eating’, is required for degradation and recycling of cytoplasmic constituents under stressful or non-stressful conditions, thereby contributing to maintaining cellular homeostasis, delaying aged and longevity in eukaryotes. So far, the functions of autophagy have been intensively studied in yeast, mammals and model plants, but few studies have focused on economic crops, especially for tea plants, the roles of autophagy in coping with different environment stimuluses have not yet been detailed. Therefore, exploring the functions of autophagy related genes in tea plant would contribute to further understanding the mechanism of autophagy in response to stresses in woody plants. Results Here, we totally identified 35 CsARGs in tea plant. Each CsARG is highly conserved with its homologues stemmed from other plant species, except for CsATG14. Tissue-specific expression analysis revealed that the abundances of CsARGs were varied with different tissues, but CsATG8c/i showed a certain degree of tissue specificity, respectively. Under hormones and abiotic stress conditions, most of CsARGs were up-regulated at different treatment time points. In addition, the transcriptions of 10 CsARGs were higher in cold-resistance cultivar ‘Longjing43’ than the cold-susceptible cultivar ‘Damianbai’ during CA periods, however, CsATG101 showed a contrary tendency. Conclusions We comprehensively analyzed the bioinformatics and physiological roles of CsARGs in tea plant, and these results provide the basis for deepen exploring the molecular mechanism of autophagy involved in tea plant growth and development and stress responses. Meanwhile, some CsARGs would be served as putative molecular markers for cold-resistance breeding of tea plant in future.

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Cloning and Expression Analysis of Auxin Efflux Carrier GeneCsPIN3in Tea Plant(Camellia sinensis)

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2016.00058 ◽

2016 ◽

Vol 42 (1) ◽

pp. 58 ◽

Cited By ~ 1

Author(s):

Bo WANG ◽

Hong-Li CAO ◽

Yu-Ting HUANG ◽

Yu-Rong HU ◽

Wen-Jun QIAN ◽

...

Keyword(s):

Expression Analysis ◽

Camellia Sinensis ◽

Tea Plant ◽

Cloning And Expression ◽

Auxin Efflux Carrier

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Advances in the knowledge of adaptive mechanisms mediating abiotic stress responses in Camellia sinensis

Frontiers in Bioscience-Landmark ◽

10.52586/5063 ◽

2021 ◽

Vol 26 (12) ◽

pp. 1714-1722

Author(s):

Yujun Sun ◽

Juan Zhou ◽

Jiansheng Guo

Keyword(s):

Abiotic Stress ◽

Camellia Sinensis ◽

Stress Responses ◽

Adaptive Mechanisms

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Genome-Wide Identification and Expression Analysis of MRLK Family Genes Associated with Strawberry (Fragaria vesca) Fruit Ripening and Abiotic Stress Responses

PLoS ONE ◽

10.1371/journal.pone.0163647 ◽

2016 ◽

Vol 11 (9) ◽

pp. e0163647 ◽

Cited By ~ 10

Author(s):

Qing Zhang ◽

Meiru Jia ◽

Yu Xing ◽

Ling Qin ◽

Bingbing Li ◽

...

Keyword(s):

Abiotic Stress ◽

Expression Analysis ◽

Stress Responses ◽

Fruit Ripening ◽

Fragaria Vesca ◽

Genome Wide

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Molecular Identification and Characterization of Hydroxycinnamoyl Transferase in Tea Plants (Camellia sinensis L.)

International Journal of Molecular Sciences ◽

10.3390/ijms19123938 ◽

2018 ◽

Vol 19 (12) ◽

pp. 3938 ◽

Cited By ~ 2

Author(s):

Chi-Hui Sun ◽

Chin-Ying Yang ◽

Jason Tzen

Keyword(s):

Abiotic Stress ◽

Amino Acid ◽

Camellia Sinensis ◽

Polymerase Chain Reaction Analysis ◽

Coffea Canephora ◽

Tea Plant ◽

Amino Acid Sequences ◽

Hibiscus Cannabinus ◽

Expression Level ◽

Tea Plants

Tea (Camellia sinensis L.) contains abundant secondary metabolites, which are regulated by numerous enzymes. Hydroxycinnamoyl transferase (HCT) is involved in the biosynthesis pathways of polyphenols and flavonoids, and it can catalyze the transfer of hydroxyconnamoyl coenzyme A to substrates such as quinate, flavanol glycoside, or anthocyanins, thus resulting in the production of chlorogenic acid or acylated flavonol glycoside. In this study, the CsHCT gene was cloned from the Chin-Shin Oolong tea plant, and its protein functions and characteristics were analyzed. The full-length cDNA of CsHCT contains 1311 base pairs and encodes 436 amino acid sequences. Amino acid sequence was highly conserved with other HCTs from Arabidopsis thaliana, Populus trichocarpa, Hibiscus cannabinus, and Coffea canephora. Quantitative real-time polymerase chain reaction analysis showed that CsHCT is highly expressed in the stem tissues of both tea plants and seedlings. The CsHCT expression level was relatively high at high altitudes. The abiotic stress experiment suggested that low temperature, drought, and high salinity induced CsHCT transcription. Furthermore, the results of hormone treatments indicated that abscisic acid (ABA) induced a considerable increase in the CsHCT expression level. This may be attributed to CsHCT involvement in abiotic stress and ABA signaling pathways.

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Genome-Wide Identification and Expression Analysis of the bZIP Transcription Factors in the Mycoparasite Coniothyrium minitans

Microorganisms ◽

10.3390/microorganisms8071045 ◽

2020 ◽

Vol 8 (7) ◽

pp. 1045

Author(s):

Yuping Xu ◽

Yongchun Wang ◽

Huizhang Zhao ◽

Mingde Wu ◽

Jing Zhang ◽

...

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Gene Family ◽

Expression Analysis ◽

Stress Responses ◽

Leucine Zipper ◽

Coniothyrium Minitans ◽

Basic Leucine Zipper ◽

Bzip Domain ◽

Conidial Development

The basic leucine zipper (bZIP) proteins family is one of the largest and most diverse transcription factors, widely distributed in eukaryotes. However, no information is available regarding the bZIP gene family in Coniothyrium minitans, an important biocontrol agent of the plant pathogen Sclerotinia sclerotiorum. In this study, we identified 34 bZIP genes from the C. minitans genome, which were classified into 8 groups based on their phylogenetic relationships. Intron analysis showed that 28 CmbZIP genes harbored a variable number of introns, and 15 of them shared a feature that intron inserted into the bZIP domain. The intron position in bZIP domain was highly conserved, which was related to recognize the arginine (R) and could be treated as a genomic imprinting. Expression analysis of the CmbZIP genes in response to abiotic stresses indicated that they might play distinct roles in abiotic stress responses. Results showed that 22 CmbZIP genes were upregulated during the later stage of conidial development. Furthermore, transcriptome analysis indicated that CmbZIP genes are involved in different stages of mycoparasitism. Among deletion mutants of four CmbZIPs (CmbZIP07, -09, -13, and -16), only ΔCmbZIP16 mutants significantly reduced its tolerance to the oxidative stress. The other mutants exhibited no significant effects on colony morphology, mycelial growth, conidiation, and mycoparasitism. Taken together, our results suggested that CmbZIP genes play important roles in the abiotic stress responses, conidial development, and mycoparasitism. These results provide comprehensive information of the CmbZIP gene family and lay the foundation for further research on the bZIP gene family regarding their biological functions and evolutionary history.

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Isolation and Characterization of CsWRKY7, a Subgroup IId WRKY Transcription Factor from Camellia sinensis, Linked to Development in Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms20112815 ◽

2019 ◽

Vol 20 (11) ◽

pp. 2815 ◽

Cited By ~ 1

Author(s):

Wei Chen ◽

Wan-Jun Hao ◽

Yan-Xia Xu ◽

Chao Zheng ◽

De-Jiang Ni ◽

...

Keyword(s):

Expression Analysis ◽

Camellia Sinensis ◽

Wrky Transcription Factor ◽

Tea Plant ◽

Wrky Gene ◽

Cis Acting ◽

Isolation And Characterization ◽

Growth Assay ◽

Meristem Identity ◽

Potential Strategy

WRKY transcription factors (TFs) containing one or two WRKY domains are a class of plant TFs that respond to diverse abiotic stresses and are associated with developmental processes. However, little has been known about the function of WRKY gene in tea plant. In this study, a subgroup IId WRKY gene CsWRKY7 was isolated from Camellia sinensis, which displayed amino acid sequence homology with Arabidopsis AtWRKY7 and AtWRKY15. Subcellular localization prediction indicated that CsWRKY7 localized to nucleus. Cis-acting elements detected in the promotor region of CsWRKY7 are mainly involved in plant response to environmental stress and growth. Consistently, expression analysis showed that CsWRKY7 transcripts responded to NaCl, mannitol, PEG, and diverse hormones treatments. Additionally, CsWRKY7 exhibited a higher accumulation both in old leaves and roots compared to bud. Seed germination and root growth assay indicated that overexpressed CsWRKY7 in transgenic Arabidopsis was not sensitive to NaCl, mannitol, PEG, and low concentration of ABA treatments. CsWRKY7 overexpressing Arabidopsis showed a late-flowering phenotype under normal conditions compared to wild type. Furthermore, gene expression analysis showed that the transcription levels of the flowering time integrator gene FLOWERING LOCUS T (FT) and the floral meristem identity genes APETALA1 (AP1) and LEAFY (LFY) were lower in WRKY7-OE than in the WT. Taken together, these findings indicate that CsWRKY7 TF may participate in plant growth. This study provides a potential strategy to breed late-blooming tea cultivar.

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