Methylobacterium oryzae Influences Isoepoxydon Dehydrogenase Gene Expression and Patulin Production by Penicillium expansum

Biofilms can be considered the main source of microorganisms in drinking water distribution systems (DWDS). The ecology of a biofilm is dependent on a variety of factors, including the presence of microbial metabolites excreted by its inhabitants. This study reports the effect of the Gram-negative bacteria Methylobacterium oryzae on the idh gene expression levels and patulin production of Penicillium expansum mature biofilms. For this purpose, a RT-qPCR method to quantify idh mRNA levels was applied. In addition, the idh expression levels were compared with the patulin production. The results obtained revealed that the effect of the bacterium on pre-established P. expansum biofilms is dependent on the time of interaction. More mature P. expansum biofilms appear to be more resistant to the inhibitory effect that M. oryzae causes towards idh gene expression and patulin production. A positive trend was observed between the idh expression and patulin production values. The results indicate that M. oryzae affects patulin production by acting at the transcriptional level of the idh gene.

Efficacy of Methylobacterium oryzae Supplemented Sargassum wightii Seaweed Liquid Fertilizer on Chilly and Tomato Plant Growth

Liquid seaweed fertilizer gaining interest due to their use in crop productivity. The present study was designed to evaluate the impact of Methylobacterium oryzae amended liquid seaweed fertilizer on domestic plants like chilly and tomato. The Sargassum wightii methanolic extract was maximum (137 mg/g) among other solvent tested. Extract showed 0.456mg/mL, 1.587 mg/mL, 0.78 mg/g, 5.27 mg/g, 0.63 mg/mL, 0.98 mg/mL, 0.285 g/mL and 0.546 μg/mL of total phenolic, total flavonoids, total chlorophyll, total carotenoids, total protein, total carbohydrates, total lipids and total aminoacids respectively. The maximum survival of Methylobacterium oryzae was observed at 40 % of Sargassum wightii SLF extract with 3% Methylobacterium oryzae culture concentration and has viability of 750 × 106 CFU/l after 6 months. Impact of foliar sprayed liquid fertilizer on the chlorophyll, internode and shoot length had been promising over seed soaked.