Quantitative Proteomic Analysis of Xanthoceras sorbifolium Bunge Seedlings in Response to Drought and Heat Stress

Abstract Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

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Quantitative proteomic analysis reveals heat stress-induced injury in rat small intestine via activation of the MAPK and NF-κB signaling pathways

Molecular BioSystems ◽

10.1039/c4mb00495g ◽

2015 ◽

Vol 11 (3) ◽

pp. 826-834 ◽

Cited By ~ 10

Author(s):

Shasha He ◽

Xiaolin Hou ◽

Xiaolong Xu ◽

Changrong Wan ◽

Peng Yin ◽

...

Keyword(s):

Small Intestine ◽

Heat Stress ◽

Signaling Pathways ◽

Proteomic Analysis ◽

Comparative Proteomics ◽

Injury Mechanism ◽

Rat Small Intestine ◽

Quantitative Proteomic Analysis

We employed comparative proteomics to reveal a heat stress-induced injury mechanism in rat small intestine.

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iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

PeerJ ◽

10.7717/peerj.11509 ◽

2021 ◽

Vol 9 ◽

pp. e11509

Author(s):

Jing Wang ◽

Chengliang Liang ◽

Sha Yang ◽

Jingshuang Song ◽

Xuefeng Li ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Proteomic Analysis ◽

Negative Impact ◽

Bioinformatic Analysis ◽

Growth And Yield ◽

Vegetable Crops ◽

Ros Scavenging ◽

Heat Stress Response ◽

Quantitative Proteomic Analysis

Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

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