iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

Abstract Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

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iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

PeerJ ◽

10.7717/peerj.11509 ◽

2021 ◽

Vol 9 ◽

pp. e11509

Author(s):

Jing Wang ◽

Chengliang Liang ◽

Sha Yang ◽

Jingshuang Song ◽

Xuefeng Li ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Proteomic Analysis ◽

Negative Impact ◽

Bioinformatic Analysis ◽

Growth And Yield ◽

Vegetable Crops ◽

Ros Scavenging ◽

Heat Stress Response ◽

Quantitative Proteomic Analysis

Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

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Quantitative Proteomic Analysis of the Heat Stress Response inClostridium difficileStrain 630

Journal of Proteome Research ◽

10.1021/pr200327t ◽

2011 ◽

Vol 10 (9) ◽

pp. 3880-3890 ◽

Cited By ~ 34

Author(s):

Shailesh Jain ◽

Ciaren Graham ◽

Robert L. J. Graham ◽

Geoff McMullan ◽

Nigel G. Ternan

Keyword(s):

Heat Stress ◽

Stress Response ◽

Proteomic Analysis ◽

Heat Stress Response ◽

Quantitative Proteomic Analysis

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Molecular mechanisms of the plant heat stress response

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2013.01.104 ◽

2013 ◽

Vol 432 (2) ◽

pp. 203-207 ◽

Cited By ~ 166

Author(s):

Ai-Li Qu ◽

Yan-Fei Ding ◽

Qiong Jiang ◽

Cheng Zhu

Keyword(s):

Heat Stress ◽

Stress Response ◽

Molecular Mechanisms ◽

Heat Stress Response

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Proteomic Analysis of Heat Stress Response in Leaves of Radish (Raphanus sativus L.)

Plant Molecular Biology Reporter ◽

10.1007/s11105-012-0486-7 ◽

2012 ◽

Vol 31 (1) ◽

pp. 195-203 ◽

Cited By ~ 36

Author(s):

Yanyu Zhang ◽

Liang Xu ◽

Xianwen Zhu ◽

Yiqin Gong ◽

Fei Xiang ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Proteomic Analysis ◽

Raphanus Sativus ◽

Heat Stress Response ◽

Raphanus Sativus L

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Quantitative Proteomic Analysis of the Response to Cold Stress in Jojoba, a Tropical Woody Crop

International Journal of Molecular Sciences ◽

10.3390/ijms20020243 ◽

2019 ◽

Vol 20 (2) ◽

pp. 243 ◽

Cited By ~ 3

Author(s):

Fei Gao ◽

Pengju Ma ◽

Yingxin Wu ◽

Yijun Zhou ◽

Genfa Zhang

Keyword(s):

Superoxide Dismutase ◽

Stress Response ◽

Cold Stress ◽

Proteomic Analysis ◽

Molecular Mechanisms ◽

Soluble Sugars ◽

Pcr Analysis ◽

Ros Scavenging ◽

Quantitative Proteomic Analysis ◽

Woody Crop

Jojoba (Simmondsia chinensis) is a semi-arid, oil-producing industrial crop that have been widely cultivated in tropical arid region. Low temperature is one of the major environmental stress that impair jojoba’s growth, development and yield and limit introduction of jojoba in the vast temperate arid areas. To get insight into the molecular mechanisms of the cold stress response of jojoba, a combined physiological and quantitative proteomic analysis was conducted. Under cold stress, the photosynthesis was repressed, the level of malondialdehyde (MDA), relative electrolyte leakage (REL), soluble sugars, superoxide dismutase (SOD) and phenylalanine ammonia-lyase (PAL) were increased in jojoba leaves. Of the 2821 proteins whose abundance were determined, a total of 109 differentially accumulated proteins (DAPs) were found and quantitative real time PCR (qRT-PCR) analysis of the coding genes for 7 randomly selected DAPs were performed for validation. The identified DAPs were involved in various physiological processes. Functional classification analysis revealed that photosynthesis, adjustment of cytoskeleton and cell wall, lipid metabolism and transport, reactive oxygen species (ROS) scavenging and carbohydrate metabolism were closely associated with the cold stress response. Some cold-induced proteins, such as cold-regulated 47 (COR47), staurosporin and temperature sensitive 3-like a (STT3a), phytyl ester synthase 1 (PES1) and copper/zinc superoxide dismutase 1, might play important roles in cold acclimation in jojoba seedlings. Our work provided important data to understand the plant response to the cold stress in tropical woody crops.

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Genetic and Molecular Mechanisms Conferring Heat Stress Tolerance in Tomato Plants

Frontiers in Plant Science ◽

10.3389/fpls.2021.786688 ◽

2021 ◽

Vol 12 ◽

Author(s):

Ken Hoshikawa ◽

Dung Pham ◽

Hiroshi Ezura ◽

Roland Schafleitner ◽

Kazuo Nakashima

Keyword(s):

Heat Stress ◽

Abiotic Stress ◽

High Temperatures ◽

Food Systems ◽

Molecular Mechanisms ◽

Plant Morphology ◽

Paris Agreement ◽

Growth And Yield ◽

Extreme Weather Events ◽

Vegetable Crops

Climate change is a major threat to global food security. Changes in climate can directly impact food systems by reducing the production and genetic diversity of crops and their wild relatives, thereby restricting future options for breeding improved varieties and reducing the ability to adapt crops to future challenges. The global surface temperature is predicted to rise by an average of 0.3°C during the next decade, and the Paris Agreement (Paris Climate Accords) aims to limit global warming to below an average of 2°C, preferably to 1.5°C compared to pre-industrial levels. Even if the goal of the Paris Agreement can be met, the predicted rise in temperatures will increase the likelihood of extreme weather events, including heatwaves, making heat stress (HS) a major global abiotic stress factor for many crops. HS can have adverse effects on plant morphology, physiology, and biochemistry during all stages of vegetative and reproductive development. In fruiting vegetables, even moderate HS reduces fruit set and yields, and high temperatures may result in poor fruit quality. In this review, we emphasize the effects of abiotic stress, especially at high temperatures, on crop plants, such as tomatoes, touching upon key processes determining plant growth and yield. Specifically, we investigated the molecular mechanisms involved in HS tolerance and the challenges of developing heat-tolerant tomato varieties. Finally, we discuss a strategy for effectively improving the heat tolerance of vegetable crops.

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