Inhibition of protein and RNA synthesis in Escherichia coli results in declustering of plasmid RK2

Plasmid ◽  
2006 ◽  
Vol 56 (2) ◽  
pp. 124-132 ◽  
Author(s):  
Shiyin Yao ◽  
Aresa Toukdarian ◽  
Donald R. Helinski
Keyword(s):  
Escherichia Coli ◽  
Rna Synthesis ◽  
Protein And Rna Synthesis ◽  
Plasmid Rk2

Relationship between ppGpp levels and rates of protein and RNA synthesis in Escherichia coli

1976 ◽  
Vol 54 (3) ◽  
pp. 291-295 ◽  
Author(s):  
Gillian Chaloner-Larsson ◽  
Hiroshi Yamazaki
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Inverse Relationship ◽  
Rna Synthesis ◽  
Amino Acid Starvation ◽  
Cellular Proteins ◽  
Mrna Species ◽  
Protein And Rna Synthesis ◽  
Rna And Protein Synthesis

When amino acid starvation is ended in stringent (relA+) strains of Escherichia coli, the rates of RNA and protein synthesis as well as their accumulation return to normal more slowly in spoT− strains than in the spoT+ strains. The level of ppGpp accumulated declines more slowly in the spoT− strains than in the spoT+ strains. Thus, there is an inverse relationship between ppGpp levels and the rates of RNA and protein synthesis. The slow resumption of protein synthesis in the spoT−relA+ strains could therefore be explained in terms of the limited synthesis of mRNA species coding for the bulk of cellular proteins.

Alternate pressurization–depressurization effects on growth and net protein, RNA and DNA synthesis by Escherichia coli and Vibrio marinus

1969 ◽  
Vol 15 (10) ◽  
pp. 1237-1240 ◽  
Author(s):  
Lawrence J. Albright
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Dna Synthesis ◽  
Rna Synthesis ◽  
Synthetic Process ◽  
E Coli ◽  
Protein And Rna Synthesis ◽  
Absorbance Changes ◽  
Pressure Resistance ◽  
Net Protein

The effects on growth and net protein, ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) synthesis of alternately pressurizing and depressurizing exponentially growing cells of Escherichia coli B/r and Vibrio marinus MP-1 between 1 atm and 544 atm of hydrostatic pressure were studied. The application of 544 atm to these 1 atm exponentially growing cultures caused an almost immediate cessation or lowering in the rates of cell division, absorbance changes, and net protein and RNA synthesis. The cells of E. coli B/r, however, in some manner adapted to 544 atm, since cell division, absorbance, and synthesis of protein and RNA, within minutes, resumed exponential increases but at much lower rates. V. marinus MP-1 did not display this pressure adaption upon the first application of 544 atm. Upon pressure release both cultures resumed the 1-atm exponential increases with respect to cell division and absorbance, and net protein and RNA synthesis. A second application of 544 atm, however, had a less drastic influence on cell division and the rates of increase of absorbance and net protein and RNA synthesis. Both cultures immediately shifted to lower synthetic rates. There was no immediate effect upon DNA synthesis in these two bacteria upon application of 544 atm, but this synthetic process slowed and ceased with time. The data indicate a reversible sensitivity to 544 atm in the order protein > RNA > DNA synthesis and that the cells can in some fashion adapt to pressure resistance.

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

scholarly journals Micro-analysis of pure deoxyribonucleic acid-dependent ribonucleic acid polymerase from Escherichia coli. Action of heparin and rifampicin on structure and function

1970 ◽  
Vol 117 (3) ◽  
pp. 623-631 ◽  
Author(s):  
Volker Neuhoff ◽  
Wolf-Bernhard Schill ◽  
Hans Sternbach
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Rna Synthesis ◽  
Deoxyribonucleic Acid ◽  
Structure And Function ◽  
Disc Electrophoresis ◽  
Inhibitory Mechanism ◽  
And Function ◽  
Micro Analysis ◽  
Template Complex

By using micro disc electrophoresis and micro-diffusion techniques, the interaction of pure DNA-dependent RNA polymerase (EC 2.7.7.6) from Escherichia coli with the template, the substrates and the inhibitors heparin and rifampicin was investigated. The following findings were obtained: (1) heparin converts the 24S and 18S particles of the polymerase into the 13S form; (2) heparin inhibits RNA synthesis by dissociating the enzyme–template complex; (3) rifampicin does not affect the attachment of heparin to the enzyme; (4) the substrates ATP and UTP are bound by enzyme loaded with rifampicin; (5) rifampicin is bound by an enzyme–template complex to the same extent as by an RNA-synthesizing enzyme–template complex. From this it is concluded that the mechanism of the inhibition of RNA synthesis by rifampicin is radically different from that by heparin. As a working hypothesis to explain the inhibitory mechanism of rifampicin, it is assumed that it becomes very firmly attached to a position close to the synthesizing site and only blocks this when no synthesis is in progress.

scholarly journals SITE OF ACTION OF 3',5'-CYCLIC ADENOSINE MONOPHOSPHATE IN PRODUCTION OF TRYPTOPHANASE IN ESCHERICHIA COLI

Genetics ◽  
1972 ◽  
Vol 70 (1) ◽  
pp. 175-180
Author(s):  
LaDonna Immken ◽  
David Apirion
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Messenger Rna ◽  
Rna Synthesis ◽  
Polypeptide Chain ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Chain Elongation ◽  
Site Of Action

ABSTRACT 3″,5″ cyclic-AMP (cAMP) will stimulate the rate of tryptophanase synthesis in Escherichia coli cultures induced with tryptophan. Adding cAMP after the initiation of messenger RNA synthesis was blocked by rifampicin, did not stimulate tryptophanase synthesis. This indicates that cAMP acts at initiation of either transcription or translation and not at the level of chain elongation of either the messenger or the polypeptide chain.

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