Plasma omega 3 polyunsaturated fatty acid status and monounsaturated fatty acids are altered by chronic social stress and predict endocrine responses to acute stress in titi monkeys

2011 ◽  
Vol 84 (3-4) ◽  
pp. 71-78 ◽  
Author(s):  
K.D. Laugero ◽  
J.T. Smilowitz ◽  
J.B. German ◽  
M.R. Jarcho ◽  
S.P. Mendoza ◽  
...  
2012 ◽  
Vol 107 (S2) ◽  
pp. S53-S63 ◽  
Author(s):  
Mario Klingler ◽  
Berthold Koletzko

Over the last few decadesn-3 long chain polyunsaturated fatty acid status became of special interest for scientists. Biochemical measures on then-3 fatty acid status vary depending on body compartment assessed and measures chosen. Plasma phospholipids and red blood cell membrane phospholipids are mainly used asn-3 fatty acid status marker. The conventional analysis of phospholipid fatty acids involves lipid extraction and consecutive chromatographic separation of phospholipids from other lipid fractions, which is time-consuming and costly. In recent years, different investigators have tried to overcome these limitations by using other biological markers or by modifying the analytical procedures used to assessn-3 fatty acid status. The aim of this systematic review was to provide an overview on these novel analytical methods developed for the fatty acid quantification by gas chromatography, highlights the methodological limitations, and discusses advantages or disadvantages of the biological markers used. Seventeen papers were identified that fulfilled the inclusion criteria. New opportunities arise from sensitive and precise high-throughput methodologies for assessment of plasma total lipid and plasma glycerophospholipid fatty acids, as well as cheek cell fatty acid composition.


2017 ◽  
Vol 117 (9) ◽  
pp. 1257-1269 ◽  
Author(s):  
Andrew J. Young ◽  
Bernadette P. Marriott ◽  
Catherine M. Champagne ◽  
Michael R. Hawes ◽  
Scott J. Montain ◽  
...  

AbstractMilitary personnel generally under-consume n-3 fatty acids and overconsume n-6 fatty acids. In a placebo-controlled, double-blinded study, we investigated whether a diet suitable for implementation in military dining facilities and civilian cafeterias could benefit n-3/n-6 fatty acid status of consumers. Three volunteer groups were provided different diets for 10 weeks. Control (CON) participants consumed meals from the US Military’s Standard Garrison Dining Facility Menu. Experimental, moderate (EXP-Mod) and experimental-high (EXP-High) participants consumed the same meals, but high n-6 fatty acid and low n-3 fatty acid containing chicken, egg, oils and food ingredients were replaced with products having less n-6 fatty acids and more n-3 fatty acids. The EXP-High participants also consumed smoothies containing 1000 mg n-3 fatty acids per serving, whereas other participants received placebo smoothies. Plasma and erythrocyte EPA and DHA in CON group remained unchanged throughout, whereas EPA, DHA and Omega-3 Index increased in EXP-Mod and EXP-High groups, and were higher than in CON group after 5 weeks. After 10 weeks, Omega-3 Index in EXP-High group had increased further. No participants exhibited changes in fasting plasma TAG, total cholesterol, LDL, HDL, mood or emotional reactivity. Replacing high linoleic acid (LA) containing foods in dining facility menus with similar high oleic acid/low LA and high n-3 fatty acid foods can improve n-6/n-3 blood fatty acid status after 5 weeks. The diets were well accepted and suitable for implementation in group feeding settings like military dining facilities and civilian cafeterias.


2018 ◽  
Vol 139 ◽  
pp. 20-24 ◽  
Author(s):  
Jesús Castro-Marrero ◽  
Maria Cleofé Zaragozá ◽  
Joan Carles Domingo ◽  
Alba Martinez-Martinez ◽  
José Alegre ◽  
...  

2020 ◽  
Vol 76 (1) ◽  
pp. 3-6
Author(s):  
Varinderpal S Dhillon ◽  
Permal Deo ◽  
Ann Chua ◽  
Phil Thomas ◽  
Michael Fenech

Abstract Lymphocyte telomere length (LTL) is a biomarker of aging that may be modified by dietary factors including fat. Red blood cell fatty acid status is a well-validated indicator of long-term dietary intake of fat from various sources. Recent findings from epidemiological studies of LTL in relation to fatty acids in red blood cells are not conclusive. The present study was carried out to investigate if red blood cell fatty acid status in 174 healthy older South Australians is associated with LTL. Lymphocyte telomere length was measured by real-time qPCR and fatty acid content in red blood cells was measured by gas chromatography. Our results indicate that the majority of saturated fatty acids and monounsaturated fatty acids are negatively associated with LTL, whereas polyunsaturated fatty acids are positively associated with LTL. Multiple regression analysis revealed that arachidonic acid (C20:4n-6) is significantly, independently, positively correlated with LTL (β = 0.262; p = .000). The significant association of fatty acids, particularly C20:4n-6, with telomere length warrants further research.


2006 ◽  
Vol 163 (6) ◽  
pp. 1100-1102 ◽  
Author(s):  
M. Elizabeth Sublette ◽  
Joseph R. Hibbeln ◽  
Hanga Galfalvy ◽  
Maria A. Oquendo ◽  
J. John Mann

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e42142 ◽  
Author(s):  
Marie Hennebelle ◽  
Laure Balasse ◽  
Alizée Latour ◽  
Gaelle Champeil-Potokar ◽  
Stéphanie Denis ◽  
...  

2012 ◽  
Vol 109 (5) ◽  
pp. 962-968 ◽  
Author(s):  
Mario Klingler ◽  
Sabrina Klem ◽  
Hans Demmelmair ◽  
Berthold Koletzko

Adequate intake of n-3 fatty acids plays an important role in human health. The analysis of various blood lipids is used as a measure of fatty acid status in humans. Cheek cell phospholipids (PL) have also been proposed as biological markers, but are rarely used in clinical studies due to limitations in sample quality and quantity. An improved method for the analysis of cheek cell glycerophospholipid fatty acids is applied in a 29 d supplementation trial with 510 mg DHA daily. The DHA increases in cheek cell, plasma and erythrocyte glycerophospholipids are compared. High correlations are shown for glycerophospholipid DHA between cheek cells and plasma (r 0·88) and erythrocytes (r 0·76) before study commencement. After the daily supplementation of DHA, the half-maximal glycerophospholipid DHA level is reached after about 4 d in plasma, 6 d in erythrocytes and 10 d in cheek cells. The mean DHA increase (mol%) relative to baseline was most prominent in plasma (186 %), followed by cheek cells (180 %) and erythrocytes (130 %). Considering a lag phase of about 5 d, cheek cells reflect short-term changes in dietary fat uptake. Based on the data of the present study, they can be used alternatively to plasma and erythrocyte PL as non-invasive n-3 fatty acid status markers.


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