Preparation, characterization, degradation and biocompatibility of different silk fibroin based composite scaffolds prepared by freeze-drying method for tissue engineering application

In the present study, poly(3-hydroxybutyrate)-based composite scaffolds were prepared with multi-walled carbon nanotubes and hydroxyapatite nanoparticles for hard tissue engineering applications by electrospinning. All the prepared scaffolds showed connective porous structure, which were suitable for cell proliferation and migration. The mechanical properties of the poly(3-hydroxybutyrate) scaffold were improved by 0.5% of carbon nanotube addition, whereas the addition of hydroxyapatite nanoparticles up to 10% had an insignificant effect in tensile strength. However, scanning electron microscopy and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results suggested that the mesenchymal stem cells attachment and their metabolic activities on the surface of the poly(3-hydroxybutyrate) scaffolds with hydroxyapatite were enhanced compared to poly(3-hydroxybutyrate) scaffolds. In addition, after 6 weeks of in vivo biocompatibility results in a model of rat indicated better tissue reactions for the scaffolds that contained hydroxyapatite. Overall, poly(3-hydroxybutyrate) composite scaffolds with 10% hydroxyapatite and 0.5% carbon nanotube showed optimal performances for the potential scaffold for hard tissue engineering application.

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Substantial effect of silk fibroin reinforcement on properties of hydroxyapatite/silk fibroin nanocomposite for bone tissue engineering application

Journal of Molecular Structure ◽

10.1016/j.molstruc.2020.127739 ◽

2020 ◽

Vol 1206 ◽

pp. 127739 ◽

Cited By ~ 5

Author(s):

J. Mobika ◽

M. Rajkumar ◽

V. Nithya Priya ◽

S.P. Linto Sibi

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Silk Fibroin ◽

Engineering Application ◽

Substantial Effect ◽

Tissue Engineering Application ◽

Bone Tissue Engineering Application

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Preparation, characterization and biocompatible properties of β-chitin/silk fibroin/nanohydroxyapatite composite scaffolds prepared using a freeze-drying method

RSC Advances ◽

10.1039/c5ra22987a ◽

2016 ◽

Vol 6 (9) ◽

pp. 7048-7060 ◽

Cited By ~ 9

Author(s):

Mohammad Azadi ◽

Abbas Teimouri ◽

Ghasem Mehranzadeh

Keyword(s):

Silk Fibroin ◽

Freeze Drying ◽

Drying Method ◽

Composite Scaffolds ◽

Organic Weight

β-Chitin/silk fibroin/nanohydroxyapatite (CT/SF/nHAp) composite scaffolds were synthesized using a freeze-drying method by blending β-chitin hydrogel, silk fibroin and nHAp at different inorganic/organic weight ratios.

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Poly(Caprolactone)/chitosan-based scaffold using freeze drying technique for bone tissue engineering application

2015 10th Asian Control Conference (ASCC) ◽

10.1109/ascc.2015.7244570 ◽

2015 ◽

Author(s):

Lor Huai Chong ◽

Nadira Zamal Zarith ◽

Naznin Sultana

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Freeze Drying ◽

Engineering Application ◽

Tissue Engineering Application ◽

Bone Tissue Engineering Application ◽

Poly Caprolactone

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Fabrication of Poly Caprolactone (PCL) Based Microspheres for Drug Delivery and Tissue Engineering Application

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.695.191 ◽

2014 ◽

Vol 695 ◽

pp. 191-194

Author(s):

Goon Chia Nyoke ◽

Naznin Sultana

Keyword(s):

Drug Delivery ◽

Tissue Engineering ◽

Freeze Drying ◽

Engineering Application ◽

Double Emulsion ◽

Synthetic Polymer ◽

Biodegradable Microspheres ◽

Tissue Engineering Application ◽

Drug Delivery Applications ◽

Poly Caprolactone

This paper reports the fabrication of poly (caprolactone) (PCL) based biodegradable microspheres using freeze-drying technique for drug delivery and tissue engineering application. Microspheres play an important role in tissue engineering and drug delivery applications. The properties can determine the success or failure of the development of the materials. Biodegradable and biocompatible synthetic polymer, PCL was used to fabricate biodegradable microspheres in this study. Through a double emulsion and freeze-drying technique, the microspheres were produced. Different concentrations of polymers were used to fabricate the microspheres. The microspheres were characterized using different techniques.

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