Preparation and performance evaluation of electrospun poly(3-hydroxybutyrate) composite scaffolds as a potential hard tissue engineering application

2019 ◽  
Vol 34 (4-5) ◽  
pp. 386-400 ◽  
Author(s):  
Moein Zarei ◽  
Nader Tanideh ◽  
Shahrokh Zare ◽  
Fatemeh Sari Aslani ◽  
Omid Koohi-Hosseinabadi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Hydroxyapatite Nanoparticles ◽  
Hard Tissue ◽  
Composite Scaffolds ◽  
Tissue Reactions ◽  
Hard Tissue Engineering

In the present study, poly(3-hydroxybutyrate)-based composite scaffolds were prepared with multi-walled carbon nanotubes and hydroxyapatite nanoparticles for hard tissue engineering applications by electrospinning. All the prepared scaffolds showed connective porous structure, which were suitable for cell proliferation and migration. The mechanical properties of the poly(3-hydroxybutyrate) scaffold were improved by 0.5% of carbon nanotube addition, whereas the addition of hydroxyapatite nanoparticles up to 10% had an insignificant effect in tensile strength. However, scanning electron microscopy and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results suggested that the mesenchymal stem cells attachment and their metabolic activities on the surface of the poly(3-hydroxybutyrate) scaffolds with hydroxyapatite were enhanced compared to poly(3-hydroxybutyrate) scaffolds. In addition, after 6 weeks of in vivo biocompatibility results in a model of rat indicated better tissue reactions for the scaffolds that contained hydroxyapatite. Overall, poly(3-hydroxybutyrate) composite scaffolds with 10% hydroxyapatite and 0.5% carbon nanotube showed optimal performances for the potential scaffold for hard tissue engineering application.

Bioactive glass containing 90% SiO 2 in hard tissue engineering: An in vitro and in vivo characterization study

2019 ◽  
Vol 13 (9) ◽  
pp. 1651-1663
Author(s):  
Luiz Felipe Cardoso Lehman ◽  
Mariana Saturnino Noronha ◽  
Ivana Márcia Alves Diniz ◽  
Rosangela Maria Ferreira Costa e Silva ◽  
Ângela Leão Andrade ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Hard Tissue ◽  
Characterization Study ◽  
In Vivo Characterization ◽  
Hard Tissue Engineering

The Mechanical Behavior of Engineered Hydrogels

2009 ◽  
Author(s):  
Audrey L. Earnshaw ◽  
Justine J. Roberts ◽  
Garret D. Nicodemus ◽  
Stephanie J. Bryant ◽  
Virginia L. Ferguson
Keyword(s):  
Tissue Engineering ◽  
Ethylene Glycol ◽  
Mechanical Behavior ◽  
Three Dimensional ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
A Chain

Agarose and poly(ethylene-glycol) (PEG) are commonly used as scaffolds for cell and tissue engineering applications [1]. Agarose is a natural biomaterial that is thought to be inert [2] and permits growing cells and tissues in a three-dimensional suspension [3]. Gels synthesized from photoreactive poly(ethylene glycol) (PEG) macromonomers are well suited as cell carriers because they can be rapidly photopolymerized in vivo by a chain radical polymerization that is not toxic to cells, including chondrocytes. This paper explores the differences in mechanical behavior between agarose, a physically cross-linked hydrogel, and PEG, a chemically cross-linked hydrogel, to set the foundation for choosing hydrogel properties and chemistries for a desired tissue engineering application.

scholarly journals Fabrication and properties of PLA/nano-HA composite scaffolds with balanced mechanical properties and biological functions for bone tissue engineering application

2021 ◽  
Vol 10 (1) ◽  
pp. 1359-1373
Author(s):  
Wenzhao Wang ◽  
Boqing Zhang ◽  
Lihong Zhao ◽  
Mingxin Li ◽  
Yanlong Han ◽  
...  
Keyword(s):  
Fused Deposition Modeling ◽  
Composite Scaffold ◽  
Engineering Application ◽  
Equal Mass ◽  
Tissue Engineering Application ◽  
Composite Scaffolds ◽  
Fused Deposition ◽  
Critical Bone Defects

Abstract Repair of critical bone defects is a challenge in the orthopedic clinic. 3D printing is an advanced personalized manufacturing technology that can accurately shape internal structures and external contours. In this study, the composite scaffolds of polylactic acid (PLA) and nano-hydroxyapatite (n-HA) were manufactured by the fused deposition modeling (FDM) technique. Equal mass PLA and n-HA were uniformly mixed to simulate the organic and inorganic phases of natural bone. The suitability of the composite scaffolds was evaluated by material characterization, mechanical property, and in vitro biocompatibility, and the osteogenesis induction in vitro was further tested. Finally, the printed scaffold was implanted into the rabbit femoral defect model to evaluate the osteogenic ability in vivo. The results showed that the composite scaffold had sufficient mechanical strength, appropriate pore size, and biocompatibility. Most importantly, the osteogenic induction performance of the composite scaffold was significantly better than that of the pure PLA scaffold. In conclusion, the PLA/n-HA scaffold is a promising composite biomaterial for bone defect repair and has excellent clinical transformation potential.

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