Structure and properties of silk fibroin aerogels prepared by non-alkali degumming process

Salt-acid system has been proved to be of high efficiency for silk fibroin dissolution. Using salt-acid system to dissolve silk, native silk fibrils can be preserved in the regenerated solution. Increasing experiments indicate that acquirement of silk fibrils in solution is strongly associated with the degumming process. In this study, the effect of sodium carbonate degumming concentration on solution properties based on lithium bromide-formic acid dissolution system was systematically investigated. Results showed that the morphology transformation of silk fibroin in solution from nanospheres to nanofibrils is determined by sodium carbonate concentration during the degumming process. Solutions containing different silk fibroin structure exhibited different rheological behaviors and different electrospinnability, leading to different electrospun nanofibre properties. The results have guiding significance for preparation and application of silk fibroin solutions.

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Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Polymers ◽

10.3390/polym11122045 ◽

2019 ◽

Vol 11 (12) ◽

pp. 2045 ◽

Cited By ~ 8

Author(s):

Guzmán Carissimi ◽

A. Abel Lozano-Pérez ◽

Mercedes G. Montalbán ◽

Salvador D. Aznar-Cervantes ◽

José Luis Cenis ◽

...

Keyword(s):

Silk Fibroin ◽

Nanoparticle Synthesis ◽

Biochemical Properties ◽

Strong Impact ◽

Lack Of Information ◽

Sds Page ◽

Silk Fibroin Nanoparticles ◽

Mean Size ◽

Degumming Process ◽

The Impact

Several studies have stated that the process used for sericin removal, or degumming, from silk cocoons has a strong impact in the silk fibroin integrity and consequently in their mechanical or biochemical properties after processing it into several biomaterials (e.g. fibers, films or scaffolds) but still, there is a lack of information of the impact on the features of silk nanoparticles. In this work, silk cocoons were degummed following four standard methods: autoclaving, short alkaline (Na2CO3) boiling, long alkaline (Na2CO3) boiling and ultrasounds. The resultant silk fibroin fibers were dissolved in the ionic liquid 1-ethyl-3-methylimidazolium acetate and used for nanoparticle synthesis by rapid desolvation in polar organic solvents. The relative efficiencies of the degumming processes and the integrity of the resulting fibroin fibers obtained were analyzed by mass loss, optical microscopy, thermogravimetric analysis, infrared spectroscopy and SDS-PAGE. Particle sizes and morphology were analyzed by Dynamic Light Scattering and Field Emission Scanning Electronic Microscopy. The results showed that the different treatments had a remarkable impact on the integrity of the silk fibroin chains, as confirmed by gel electrophoresis, which can be correlated with particle mean size and size distribution changes. The smallest nanoparticles (156 ± 3 nm) and the most negative Z potential (−30.2 ± 1.8 mV) were obtained with the combination of long treatment (2 h) of boiling in alkaline solution (Na2CO3 0.02 eq/L). The study confirms that parameters of the process, such as composition of the solution and time of the degumming step, must be controlled in order to reach an optimum reproducibility of the nanoparticle production.

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In vitro studies on the structure and properties of silk fibroin aqueous solutions in silkworm

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2013.08.027 ◽

2013 ◽

Vol 62 ◽

pp. 162-166 ◽

Cited By ~ 17

Author(s):

Yuan Jin ◽

Yichun Hang ◽

Jie Luo ◽

Yaopeng Zhang ◽

Huili Shao ◽

...

Keyword(s):

Aqueous Solutions ◽

Silk Fibroin ◽

In Vitro Studies ◽

Structure And Properties

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An administration on artificial skin for burning patient skin recovery through tissue engineering

Current Applied Polymer Science ◽

10.2174/2452271604999201005163920 ◽

2020 ◽

Vol 04 ◽

Author(s):

Sourav Kumar Das

Keyword(s):

Tissue Engineering ◽

Cell Culture ◽

Silk Fibroin ◽

Human Body ◽

Research Work ◽

Collagen Scaffold ◽

Artificial Skin ◽

Skin Substitutes ◽

Degumming Process

Background: Artificial skin has been tried to implement on subject which is damaged by burn effect.In this research i tried with Silk fibroin as a block biopolymer and which is very high biodegradability and more flexible for cell culture as being a part of human body.With this artificial skin of SF/Chitosan will help to produce more pores of tissue engineering which will create a good implantation system.Skin is the longest and most important organ of human body.Artificial skin is a collagen scaffold that causes skin regeneration in animals such as humans. The innovative biomaterial silk consists of this collagen.I also tried to introduce collagen consisting of silk nanofibers for human body linked to burning patients impacted areas via tissue engineering administration.Body comprises of three layers.They are sometimes called the hypodermis, epidermis, dermis and the fat layer. Purpose: An exploration to implement human compatible artificial skin for burned body. Materials and methods: At first turned silk fibroin through degumming process as a block biopolymer and then check oxygen permeability with silk fibroin and chitosan combination.Check mechanical properties and this total experiment done through tissue engineering and cell culture. Tissue-built skin substitutes may offer a viable helpful choice for the treatment of patients with skin harms. Results: Implementable long term sustainability and high biodegradability artificial skin through cell culture. Conclusion: In this research, artificial skin has been developed with chitosan/SF layer based with complete block biopolymer and i got a significant result which will be compatible for human original skin with high oxygen and water permeability with cell culture and it is effective and result oriented for burning patient skin recovery and accidental case in the replacement of skin. Discussion: Artificial skin is a collagen scaffold that induces regeneration of skin in mammals such as humans.The novel biomaterial silk consists of this collagen.In this research work,we tried to implement collagen consisting silk nanofibers for artificial skin for human body related to burning patients affected areas through tissue engineering administration.

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Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

Materials Research Express ◽

10.1088/2053-1591/ab69c4 ◽

2020 ◽

Vol 7 (1) ◽

pp. 015413 ◽

Cited By ~ 1

Author(s):

Ruixia Hou ◽

Yuxi Wang ◽

Jiali Han ◽

Yabin Zhu ◽

Hua Zhang ◽

...

Keyword(s):

Silk Fibroin ◽

Cell Types ◽

Growth Behavior ◽

Structure And Properties

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Effect of Glycerol on Structure and Properties of Silk Fibroin/Pearl Powder Blend Films

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.796.126 ◽

2013 ◽

Vol 796 ◽

pp. 126-131 ◽

Cited By ~ 4

Author(s):

Rui Juan Xie ◽

Meng Zhang

Keyword(s):

Dissolution Rate ◽

Silk Fibroin ◽

Testing Machine ◽

Amorphous Structure ◽

Structure And Properties ◽

X Ray Diffraction ◽

Elongation At Break ◽

Blend Films ◽

Universal Testing ◽

Significant Difference

In the present study, glycerol was used as plasticizer to prepare silk fibroin (SF)/pearl powder (PP) blend films. The effects of amount of glycerol on structure and properties of the films were investigated. The surface morphology was observed with scanning electron microscopy. The structure of films was investigated by X-ray diffraction and thermal analysis. The mechanical properties of the films were measured on a universal testing machine, and the dissolution rate of SF was examined by ultraviolet spectroscopy. The results showed that surface of pure SF films was smooth, but the surface of films containingPP was uneven, particles of PP dispersed in the films. The structure of the film without glycerol was mainly amorphous structure. The structure of the SF in the film was mainly silk I and silk II when the proportion of glycerol added was in the range of 10%- 20%, while the main structure of the SF in the films was silk I when the proportion of glycerol was more than 20%. The dissolution rate of SF in films without glycerol is rather great, while the dissolution rate had a significant decrease by adding glycerol. There was no significant difference in dissolution rates of SF which were all below 1.3% when the proportion of glycerol is 10-40%. The films without glycerol had very small elongation at break. The elongation at break of SF films increased with the increased amount of glycerol. Compared to films without glycerol, there was a significant difference when the proportion of glycerol was greater than 10%. The tensile strength of the films dropped significantly with the increase of glycerol, but there was no significant difference when the proportion of glycerol was greater than 30%. Therefore, the advisable addition percentage of glycerol is 20%.

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