The effectiveness of pyrimethanil to inhibit germination of Penicillium digitatum and to control citrus green mold after harvest

2006 ◽  
Vol 42 (1) ◽  
pp. 75-85 ◽  
Author(s):  
J.L. Smilanick ◽  
M.F. Mansour ◽  
F. Mlikota Gabler ◽  
W.R. Goodwine
2022 ◽  
Vol 22 (1) ◽  
pp. 142-150
Author(s):  
Redouan Qessaoui ◽  
Mariem Zanzan ◽  
Abdelhadi Ajerrar ◽  
Hind Lahmyed ◽  
Ahmed Boumair ◽  
...  

Plant Disease ◽  
2006 ◽  
Vol 90 (6) ◽  
pp. 765-770 ◽  
Author(s):  
Leigh S. Schmidt ◽  
Jennifer M. Ghosoph ◽  
Dennis A. Margosan ◽  
Joseph L. Smilanick

Thiabendazole (TBZ) is commonly applied to harvested citrus fruit in packinghouses to control citrus green mold, caused by Penicillium digitatum. Although TBZ is not used before harvest, another benzimidazole, thiophanate methyl, is commonly used in Florida and may be introduced soon in California to control postharvest decay of citrus fruit. Isolates from infected lemons and oranges were collected from many geographically diverse locations in California. Thirty-five isolates collected from commercial groves and residential trees were sensitive to TBZ, while 19 of 74 isolates collected from 10 packinghouses were resistant to TBZ. Random amplified polymorphic DNA analysis indicated that the isolates were genetically distinct and differed from each other. Nineteen TBZ-resistant isolates and a known TBZ-resistant isolate displayed a point mutation in the β-tubulin gene sequence corresponding to amino acid codon position 200. Thymine was replaced by adenine (TTC → TAC), which changed the phenylalanine (F) to tyrosine (Y). In contrast, for 49 TBZ-sensitive isolates that were sequenced, no mutations at this or any other codon positions were found. All of the isolates of P. digitatum resistant to TBZ collected from a geographically diverse sample of California packinghouses appeared to have the same point mutation conferring thiabendazole resistance.


2000 ◽  
Vol 90 (9) ◽  
pp. 932-943 ◽  
Author(s):  
Nicole Benhamou ◽  
Jacques Brodeur

Chronological events of the intercellular interaction between Verticillium lecanii and the postharvest pathogen Penicillium digitatum were investigated by transmission electron microscopy and gold cytochemistry. Growth inhibition of P. oligandrum as a response to V. lecanii attack correlated with striking host changes including retraction of the plasma membrane and cytoplasm disorganization. Such changes were associated with the deposition on the inner host cell surface of a chitin- and cellulose-enriched material which appeared to be laid down as a structural defense reaction. The accumulation of chitin in the newly formed material correlated with a decrease in the amount of wallbound chitin. However, the deposition of cellulose appeared to correspond to a de novo synthesis, as evidenced by the occurrence of cellulose-containing vesicles which released their content in the space between the invaginated plasma membrane and the host cell wall. Results of the present study provide the first ultrastructural and cytochemical evidence that antagonism, triggered by V. lecanii, is a multifaceted process in which antibiosis, with alteration of the host hyphae prior to contact with the antagonist, appears to be the key process in the antagonism against P. digitatum.


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 604e-604
Author(s):  
John E. Fucik

The harvest of Rio Red grapefruit (Citrus paradisi Macf.) was “intercepted” at three stages: 1) unpicked fruit, 2) picked and carried to pallet box trailer, and 3) picked, carried, dumped in the pallet box and transported to the packing shed. Three harvesters picked fruit from four canopy locations on two trees each. At each intercept, half the fruit was dipped into a spore solution of green mold (Penicillium digitatum) and half left nontreated as controls. Intercept 1 fruit was dipped and left unpicked on the tree. After 10 days incubation, the rate of green mold infection and its location on the fruit was determined. Tests were run in May 1995 and Feb. and Apr. 1996. The rate of infection increased with each intercept, and treated fruit had 15 times the infection rate of the controls. The highest infection rate, 1.3%, occurred in May 1995 followed by Feb. (0.8%), and April (0.5%). Most infection sites appeared above and below the fruit's equator, rather than on its top or bottom exclusively. There were no effects associated with harvesters or the location of the fruit in the canopy.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Shuhua Lin ◽  
Yuanxiu Wang ◽  
Qunlin Lu ◽  
Bin Zhang ◽  
Xiaoyu Wu

AbstractPenicillium digitatum is the primary spoilage fungus that causes green mold during postharvest in citrus. To reduce economic losses, developing more efficient and less toxic natural antimicrobial agents is urgently required. We previously found that the X33 antimicrobial oligopeptide (X33 AMOP), produced by Streptomyces lavendulae X33, exhibited a sterilization effect on P. digitatum. In this study, the effects, and physiological mechanisms of X33 AMOP as an inhibitor of P. digitatum were investigated. The transcriptional and metabolome profiling of P. digitatum exposed to X33 AMOP revealed 3648 genes and 190 metabolites that were prominently changed. The omics analyses suggested that X33 AMOP mainly inhibited P. digitatum growth by affecting cell integrity, genetic information delivery, oxidative stress tolerance, and energy metabolism. These findings provide helpful information regarding the antimicrobial mechanism of X33 AMOP against P. digitatum at the molecular level and indicate that X33 AMOP is a potential candidate to control P. digitatum. Graphical Abstract


2020 ◽  
Vol 8 (10) ◽  
pp. 1477 ◽  
Author(s):  
Mariana Andrea Díaz ◽  
Martina María Pereyra ◽  
Fabricio Fabián Soliz Santander ◽  
María Florencia Perez ◽  
Josefina María Córdoba ◽  
...  

Fungal rots are one of the main causes of large economic losses and deterioration in the quality and nutrient composition of fruits during the postharvest stage. The yeast Clavispora lusitaniae 146 has previously been shown to efficiently protect lemons from green mold caused by Penicillium digitatum. In this work, the effect of yeast concentration and exposure time on biocontrol efficiency was assessed; the protection of various citrus fruits against P. digitatum by C. lusitaniae 146 was evaluated; the ability of strain 146 to degrade mycotoxin patulin was tested; and the effect of the treatment on the sensory properties of fruits was determined. An efficient protection of lemons was achieved after minimum exposure to a relatively low yeast cell concentration. Apart from lemons, the yeast prevented green mold in grapefruits, mandarins, oranges, and tangerines, implying that it can be used as a broad-range biocontrol agent in citrus. The ability to degrade patulin indicated that strain 146 may be suitable for the control of further Penicillium species. Yeast treatment did not alter the sensory perception of the aroma of fruits. These results corroborate the potential of C. lusitaniae 146 for the control of postharvest diseases of citrus fruits and indicate its suitability for industrial-scale fruit processing.


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