Biocontrol Bacteria Strains Y4 and Y8 Alleviate Tobacco Bacterial Wilt Disease by Altering their Rhizosphere Soil Bacteria Community

Rhizosphere ◽  
2021 ◽  
pp. 100390
Author(s):  
Junying Li ◽  
Qiqi Zhao ◽  
Hada Wuriyanghan ◽  
Chao Yang
Keyword(s):  
Bacterial Wilt ◽  
Soil Bacteria ◽  
Rhizosphere Soil ◽  
Wilt Disease ◽  
Bacterial Wilt Disease ◽  
Biocontrol Bacteria

scholarly journals Bacterial Tomato Pathogen Ralstonia solanacearum Invasion Modulates Rhizosphere Compounds and Facilitates the Cascade Effect of Fungal Pathogen Fusarium solani

2020 ◽  
Vol 8 (6) ◽  
pp. 806
Author(s):  
Lv Su ◽  
Lifan Zhang ◽  
Duoqian Nie ◽  
Eiko E. Kuramae ◽  
Biao Shen ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Bacterial Wilt ◽  
Fusarium Solani ◽  
Ralstonia Solanacearum ◽  
High Throughput Sequencing ◽  
Rhizosphere Soil ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Pathogen Invasion

Soil-borne pathogen invasions can significantly change the microbial communities of the host rhizosphere. However, whether bacterial Ralstonia solanacearum pathogen invasion influences the abundance of fungal pathogens remains unclear. In this study, we combined high-throughput sequencing, qPCR, liquid chromatography and soil culture experiments to analyze the rhizosphere fungal composition, co-occurrence of fungal communities, copy numbers of functional genes, contents of phenolic acids and their associations in healthy and bacterial wilt-diseased tomato plants. We found that R. solanacearum invasion increased the abundance of the soil-borne pathogen Fusarium solani. The concentrations of three phenolic acids in the rhizosphere soil of bacterial wilt-diseased tomato plants were significantly higher than those in the rhizosphere soil of healthy tomato plants. In addition, the increased concentrations of phenolic acids significantly stimulated F. solani growth in the soil. Furthermore, a simple fungal network with fewer links, nodes and hubs (highly connected nodes) was found in the diseased tomato plant rhizosphere. These results indicate that once the symptom of bacterial wilt disease is observed in tomato, the roots of the wilt-diseased tomato plants need to be removed in a timely manner to prevent the enrichment of other fungal soil-borne pathogens. These findings provide some ecological clues for the mixed co-occurrence of bacterial wilt disease and other fungal soil-borne diseases.

Evaluation of seed associated endophytic bacteria from tolerant chilli cv. Firingi Jolokia for their biocontrol potential against bacterial wilt disease

2021 ◽  
pp. 126751
Author(s):  
Bhaskar Dowarah ◽  
Heena Agarwal ◽  
Debasish B Krishnatreya ◽  
Pankaj Losan Sharma ◽  
Nilamjyoti Kalita ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Endophytic Bacteria ◽  
Wilt Disease ◽  
Bacterial Wilt Disease ◽  
Biocontrol Potential

scholarly journals An Antimicrobial Substance Produced by Pseudomonas cepacia B5 against the Bacterial Wilt Disease Pathogen, Pseudomonas solanacearum

1991 ◽  
Vol 55 (3) ◽  
pp. 715-722 ◽  
Author(s):  
Michiko AOKI ◽  
Kyoko UEHARA ◽  
Koshi KOSEKI ◽  
Kazumasa TSUJI ◽  
Masaharu IIJIMA ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Wilt Disease ◽  
Pseudomonas Cepacia ◽  
Antimicrobial Substance ◽  
Pseudomonas Solanacearum ◽  
Bacterial Wilt Disease

Genetics of tolerance to bacterial wilt disease in tomato (Solanum lycopersicum L.)

2018 ◽  
Vol 47 (6) ◽  
pp. 591-600
Author(s):  
Brati Acharya ◽  
Ankit Kumar Ghorai ◽  
Subhramalya Dutta ◽  
Praveen Kumar Maurya ◽  
Subrata Dutta ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Bacterial Wilt ◽  
Wilt Disease ◽  
Bacterial Wilt Disease ◽  
Solanum Lycopersicum L

scholarly journals Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato

Mycobiology ◽  
2015 ◽  
Vol 43 (3) ◽  
pp. 311-318 ◽  
Author(s):  
A Min Kwak ◽  
Kyeong Jin Min ◽  
Sang Yeop Lee ◽  
Hee Wan Kang
Keyword(s):  
Bacterial Wilt ◽  
Water Extract ◽  
Wilt Disease ◽  
Spent Mushroom Substrate ◽  
Hericium Erinaceus ◽  
Bacterial Wilt Disease

scholarly journals Trehalose induces tomato defenses and increases drought tolerance and bacterial wilt disease resistance

2021 ◽  
Author(s):  
April M MacIntyre ◽  
Valerian Meline ◽  
Zachary Gorman ◽  
Steven P Augustine ◽  
Carolyn J Dye ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Stomatal Conductance ◽  
Water Use ◽  
Bacterial Wilt ◽  
Xylem Sap ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Infected Tomato ◽  
Use Efficiency

Ralstonia solanacearum causes plant bacterial wilt disease, leading to severe crop losses. Xylem sap from R. solanacearum-infected tomato is enriched in host produced trehalose. Water stressed plants accumulate the disaccharide trehalose, which increases drought tolerance via abscisic acid (ABA) signaling networks. Because infected plants have reduced water flow, we hypothesized that bacterial wilt physiologically mimics drought stress, which trehalose could mitigate. Transcriptomic responses of susceptible vs. resistant tomato plants to R. solanacearum infection revealed differential expression of drought-associated genes, including those involved in ABA and trehalose metabolism. ABA was enriched in xylem sap from R. solanacearum-infected plants. Treating roots with ABA lowered stomatal conductance and reduced R. solanacearum stem colonization. Treating roots with trehalose increased ABA in xylem sap and reduced plant water use by reducing stomatal conductance and temporarily improving water use efficiency. Further, trehalose-treated plants were more resistant to bacterial wilt disease. Trehalose treatment also upregulated expression of salicylic acid (SA)-dependent defense genes, increased xylem sap levels of SA and other antimicrobial compounds, and increased wilt resistance of SA-insensitive NahG tomato plants. Additionally, trehalose treatment increased xylem concentrations of jasmonic acid and related oxylipins. Together, these data show that exogenous trehalose reduced both water stress and bacterial wilt disease and triggered systemic resistance. This suite of responses revealed unexpected linkages between plant responses to biotic and abiotic stress and suggests that that R. solanacearum-infected tomato plants produce more trehalose to improve water use efficiency and increase wilt disease resistance. In turn, R. solanacearum degrades trehalose as a counter-defense.

scholarly journals Revisiting the source of wilt symptoms: X-ray microcomputed tomography provides direct evidence that Ralstonia biomass clogs xylem vessels

2021 ◽  
Author(s):  
Brian Ingel ◽  
Denise Caldwell ◽  
Fiona Duong ◽  
Dilworth Y. Parkinson ◽  
Katherine A. McCulloh ◽  
...  
Keyword(s):  
Stomatal Conductance ◽  
Light Microscopy ◽  
Bacterial Wilt ◽  
Xylem Sap ◽  
Physiological Mechanism ◽  
Bacterial Biomass ◽  
Microcomputed Tomography ◽  
Wilt Disease ◽  
X Ray ◽  
Bacterial Wilt Disease

AbstractPlant pathogenic Ralstonia cause wilt diseases by colonizing xylem vessels and disrupting water transport. Due to the abundance of Ralstonia cells in vessels, the dogma is that bacterial biomass clogs vessels and reduces the flow of xylem sap. However, the physiological mechanism of xylem disruption during bacterial wilt disease is untested. Using a tomato and Ralstonia pseudosolanacearum GMI1000 model, we visualized and quantified the spatiotemporal dynamics of xylem disruption during bacterial wilt disease. First, we measured stomatal conductance of leaflets on mock-inoculated and wilt-symptomatic plants. Wilted leaflets had reduced stomatal conductance, as did turgid leaflets located on the same petiole as wilted leaflets. Next, we used X-ray microcomputed tomography (X-ray microCT) and light microscopy to differentiate between mechanisms of xylem disruption: blockage by bacterial biomass, blockage by vascular tyloses, or sap displacement by gas embolisms. We imaged stems on plants with intact roots and leaves to quantify embolized vessels. Embolized vessels were rare, but there was a slight trend of increased vessel embolisms in infected plants with low bacterial population sizes. To test the hypothesis that vessels are clogged during bacterial wilt, we imaged excised stems after allowing the sap to evaporate during a brief dehydration. Most xylem vessels in mock-infected plants emptied their contents after excision, but non-conductive clogged vessels were abundant in infected plants by 2 days post infection. At wilt onset when bacterial populations exceeded 5×108 cfu/g stem tissue, approximately half of the xylem vessels were clogged with electron-dense bacterial biomass. We found no evidence of tyloses in the X-ray microCT reconstructions or light microscopy on the preserved stems. Bacterial blockage of vessels appears to be the principal cause of vascular disruption during Ralstonia wilt.

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