scholarly journals Green synthesis of silver nanoparticles using a Melissa officinalis leaf extract with antibacterial properties

2017 ◽  
Vol 7 ◽  
pp. 2639-2643 ◽  
Author(s):  
Álvaro de Jesús Ruíz-Baltazar ◽  
Simón Yobbany Reyes-López ◽  
Daniel Larrañaga ◽  
Miriam Estévez ◽  
Ramiro Pérez
2020 ◽  
Vol 11 (03) ◽  
pp. 379-388
Author(s):  
Anto Cordelia T. A. Dhanapal ◽  
Ashwinie N. Warrant

The leaf extract of O. tenuiflorum was used to synthesize silver nanoparticles (AgNPs) and evaluated for its antioxidant and antibacterial properties. The silver nanoparticle was characterized using the UV-Vis spectrophotometer, SEM, FTIR, and XRD. The total phenolic and total flavonoid contents were determined for both leaf extract and synthesized silver nanoparticles. Antioxidant activities before and after synthesis of silver nanoparticles was assessed by DPPH, ABTS, iron chelating, and NO radical scavenging methods. The antibacterial activity of the leaf extract and AgNP were tested against Escherichia coli and Staphylococcus aureus. Statistical analysis was carried out to establish possible relations between the antioxidant, antibacterial and antioxidant activities. The formation of a dark brown solution mixture confirms the formation of silver nanoparticles at a wavelength of 450 nm. The AgNPs synthesized were spherical, with the size between 14 to 33 nm. Functional groups such as alcohol, aldehyde, nitrile, primary amines, carbonyl, and aromatic groups were confirmed by FTIR and XRD. Total phenol was higher in leaf extract, while total flavonoids were higher in the AgNps. Silver nanoparticles exhibited strong NO scavenging activity while leaf extract showed better ABTS scavenging activity. Silver nanoparticles inhibited E. coli better compared to S. aureus bacteria. It can be coined that the leaf extract of Ocimum tenuiflorum mediated the green synthesis of silver nanoparticles and possess strong antioxidant and antibacterial potentials that can find application in various biomedical areas.


Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4041
Author(s):  
Adriana Cecilia Csakvari ◽  
Cristian Moisa ◽  
Dana G. Radu ◽  
Leonard M. Olariu ◽  
Andreea I. Lupitu ◽  
...  

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.


2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akshay Rajeev Geetha ◽  
Elizabeth George ◽  
Akshay Srinivasan ◽  
Jameel Shaik

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.


Author(s):  
Joy James Costa ◽  
Hassan Hosseinzadeh ◽  
Dabasish Kumar Saha ◽  
Shihab Uddin Al Mahmud ◽  
Bhuiyan Mohammad Mahtab Uddin ◽  
...  

2018 ◽  
Vol 772 ◽  
pp. 73-77
Author(s):  
Ruelson S. Solidum ◽  
Arnold C. Alguno ◽  
Rey Capangpangan

We report on the green synthesis of silver nanoparticles utilizing theP.purpureumleaf extract. Controlling the surface plasmon absorption of silver nanoparticles was achieved by regulating the amount of extract concentration and the molarity of silver nitrate solution. The surface plasmon absorption peak is found at around 430nm. The surface plasmon absorption peak have shifted to lower wavelength as the amount of extract is increased, while plasmon absorption peak shifts on a higher wavelength as the concentration of silver nitrate is increased before it stabilized at 430nm. This can be explained in terms of the available nucleation sites promoted by the plant extract as well as the available silver ions present in silver nitrate solution.


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