Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.

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Optimization of Green Synthesis of Silver Nanoparticles from Leaf Extracts ofPimenta dioica(Allspice)

The Scientific World JOURNAL ◽

10.1155/2013/362890 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 5

Author(s):

Akshay Rajeev Geetha ◽

Elizabeth George ◽

Akshay Srinivasan ◽

Jameel Shaik

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Leaf Extract ◽

Synthesis Method ◽

Leaf Extracts ◽

Synthesis Of Nanoparticles ◽

Hot Air ◽

Vis Spectroscopy ◽

Pimenta Dioica ◽

First Time

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.

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Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

Sustainability ◽

10.3390/su12041484 ◽

2020 ◽

Vol 12 (4) ◽

pp. 1484 ◽

Cited By ~ 3

Author(s):

M. Asimuddin ◽

Mohammed Rafi Shaik ◽

Neeshat Fathima ◽

M. Shaistha Afreen ◽

Syed Farooq Adil ◽

...

Keyword(s):

Silver Nanoparticles ◽

Metallic Nanoparticles ◽

Low Cost ◽

Preliminary Investigation ◽

Antibacterial Properties ◽

Silver Ions ◽

Ziziphus Mauritiana ◽

Bacterial Strains ◽

Vis Spectroscopy ◽

Ft Ir

Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

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Green synthesis and antibacterial activity studies of silver nanoparticles from the aqueous extracts of Thespesia populnea

Letters in Applied NanoBioScience ◽

10.33263/lianbs91.931934 ◽

2020 ◽

Vol 9 (1) ◽

pp. 931-934

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Inhibitory Concentration ◽

Xrd Analysis ◽

Leaf Extracts ◽

Swarming Motility ◽

Thespesia Populnea ◽

Vis Spectroscopy ◽

Test Organisms

Green synthesis of silver nanoparticles were carried out using the aqueous leaf extracts of Thespesia populnea and characterised by UV Vis spectroscopy, SEM, EDX, AFM and XRD analysis. The silver nanoparticles when tested on Pseudomonas aeruginosa and Bacillus subtilis, was found to have potent antibacterial activity. Further, the microbes were susceptible for a minimum inhibitory concentration of 0.5 µg and also arrested the swarming motility of the test organisms. The nanoparticles interacted with the microbial membrane which led to the leakage of proteins from the cells.

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Green Synthesis of Ag Nanoparticles for Anti-Microbial Applications using Aloe Barbadensis Miller Extract

International Journal of Advanced Research in Science, Communication and Technology ◽

10.48175/ijarsct-1230 ◽

2021 ◽

pp. 233-239

Author(s):

Sankar S ◽

Ganga Krishnan

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Ag Nanoparticles ◽

Aloe Vera ◽

Silver Ions ◽

Xrd Analysis ◽

Aloe Barbadensis ◽

Agno3 Solution ◽

Vis Spectroscopy ◽

Ft Ir

The green synthesis of nanomaterials is becoming much popular as a result of worldwide problems associated with environmental concerns. In the present work, leaf extract of Barbadensis Miller, (commonly known as Aloe vera) was used for bio-reduction of silver ions to silver nanoparticles. Aloe vera extract and AgNO3 solution in different volumes were treated and it resulted in the reduction of Ag+ ions to Ag metal atoms, which further accumulated as Ag nanoparticles. The prepared nanoparticles were characterized by UV-vis spectroscopy, SEM, FT-IR spectroscopy and XRD analysis. The present study established that the shape and size of the silver nanoparticles can be effectively controlled and modulated using green synthesis technique. The scope of the prepared particles for anti-microbial applications were also investigated.

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GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM FRUIT RIND EXTRACT OF GARCINIA MANGOSTANA L. AND EVALUATION OF ANTIBACTERIAL PROPERTIES

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.no2.043 ◽

2021 ◽

Vol 21 (2) ◽

Author(s):

Manju Madhavan ◽

Elsa Rose P.J. ◽

Arunima Francis ◽

Grace Maria Benny ◽

Anila Wilson

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Wave Length ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Diffusion Method ◽

Toxic Substances ◽

Garcinia Mangostana ◽

Vis Spectroscopy ◽

Fruit Rind

Nanotechnology is an emerging area of science that involves the engineering of nano sized particles of various materials. Among the various nanoparticles, the silver nanoparticles are used in various applications due to their unique characters. The production of silver nanoparticles using a chemical method is harmful and produces toxic substances as by-products, so the aim of our study was to green synthesis silver nanoparticles using the fruit rind of Garcinia mangostana L. which is being discarded as a waste material. Aqueous rind extract of G. mangostana was prepared and synthesis of silver nanoparticles was analysed by UV-Vis spectrophotometer by optimizing various parameters like pH, time and concentration of extract. The nanoparticles were characterized using UV-Vis spectroscopy and the peak was obtained between the wave length of 410 - 433 nm in various treatments. The antibacterial activities of synthesized silver nanoparticles were tested against both gram negative (Pseudomonas) and gram positive (Staphylococcus) bacteria using the well diffusion method. The aqueous extract shows the remarkable zone of inhibition against Pseudomonas and Staphylococcus.

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Synthesis of Ag-NPs from extracts of Persea americana and its antimicrobial effects in human pathogens

10.34256/nnxt2012 ◽

2020 ◽

Vol 1 (1) ◽

pp. 10-17

Author(s):

Selvam K.A ◽

◽

Suriyakumar M ◽

Devanathan J ◽

Kolanjinathan K ◽

...

Keyword(s):

Silver Nanoparticles ◽

Streptococcus Mutans ◽

Persea Americana ◽

Human Beings ◽

Human Pathogens ◽

Zone Of Inhibition ◽

Ag Nps ◽

Leaf Extracts ◽

Agar Disc ◽

Vis Spectroscopy

Medicines of plant origin have been used to combat human diseases since time immemorial even before the history of their documentation. They have been used by human beings since time immemorial for curing health. The phytochemical screening of leaf extracts of Persea americanashows the presence of alkaloids, flavanoids, carbohydrates, saponins, protein, tannins and glycoside. Also the synthesis of silver nanoparticles was done using ethanolic Persea americana leaf extract. The synthesized silver nanoparticles were characterized by using Ultraviolet- visible (UV-Vis) spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis. The antimicrobial potential of synthesized nanoparticles was determined on the basis of mean diameter of zone of inhibition around the disc in millimeters. The antibacterial activity was evaluated qualitatively through agar disc diffusion towards Lactobacillus sp, Staphylococcus aureus, Streptococcus mutans, Klebsiella pneumoniae, Serratia marcescens, and Escherichia coli. The highest zone of inhibition value (15.0 mm) in Streptococcus mutans 15 ± 0.6. The antifungal activity against Aspergillus niger, Aspergillus flaves ,Candida albicans and Fusarium sp. From the results, excellent and eco-friendly green source for production of potential bio antimicrobial silver nanoparticles.

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Green Synthesis of Antimicrobial Silver Nanoparticles using Fruit Extract of Glycosmis Pentaphylla and its Theoretical Explanations.

10.21203/rs.3.rs-200301/v1 ◽

2021 ◽

Author(s):

Tanmoy Dutta ◽

Swapan Kumar Chowdhury ◽

Narendra Nath Ghosh ◽

Mahuya Das ◽

Asoke P. Chattopadhyay ◽

...

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Density Functional ◽

Cost Effective ◽

Human Pathogens ◽

Fruit Extract ◽

One Pot ◽

Theoretical Simulation ◽

Vis Spectroscopy ◽

Average Size

Abstract The present study reports a novel, one-pot, cost-effective, green synthesis route of silver nanoparticles (AgNPs) from the fruit extract of Glycosmis pentaphylla (FGP). The UV–vis spectroscopy (UV-Vis), dynamic light scattering (DLS) and transmission electron microscopy (TEM) studies confirmed that the synthesis produces stable, monodispersed AgNPs with an average size of 17 nm. Theoretical simulation using density functional theory (DFT) established that among the different compounds of FGP, arborine is mainly responsible for the stabilization of AgNPs with a binding energy of 58.45 kJ/mol. Synthesized AgNPs showed strong antifungal and antibacterial activity. The synergistic study of AgNPs with fungicide Bavistin and antibiotic Streptomycin produced remarkable morphological abnormalities of A. alternata as observed under the light microscope. Hence, the AgNPs synthesis approach is a progressive step towards various applications to soon control crop and human pathogens.

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Green Synthesis of Silver Nanoparticles Using Apple Extract and Its Antibacterial Properties

Advances in Materials Science and Engineering ◽

10.1155/2016/4102196 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 28

Author(s):

Zainal Abidin Ali ◽

Rosiyah Yahya ◽

Shamala Devi Sekaran ◽

R. Puteh

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Color Change ◽

Antibacterial Properties ◽

Reducing Agent ◽

X Ray Diffraction ◽

Gram Positive Bacteria ◽

Clean Synthesis ◽

Vis Spectroscopy ◽

Ag Crystallites

Silver nanoparticles (AgNPs) were synthesized using apple extract as a reducing agent and aqueous silver nitrate as the precursor. The AgNPs formation was observed as a color change of the mixture from colorless to dark-brownish. The X-ray diffraction pattern confirmed the presence of only Ag crystallites, and the dynamic light scattering estimates the average sizes of the AgNPs to be 30.25 ± 5.26 nm. Furthermore, Fourier Transform Infrared as well as UV-vis spectroscopy identifies ethylene groups as the reducing agent and capping agent for the formation of the AgNPs. This green synthesis provides an economic, eco-friendly, and clean synthesis route to AgNPs. AgNPs in suspension showed activity against Gram-negative and Gram-positive bacteria with minimum bactericidal concentrations (MBCs) to be in the range from 125 μg/mL to 1000 μg/mL.

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