scholarly journals Molecular detection and isolation of pathogenic Leptospira from asymptomatic humans, domestic animals and water sources in Nan province, a rural area of Thailand

2017 ◽  
Vol 115 ◽  
pp. 146-154 ◽  
Author(s):  
Alongkorn Kurilung ◽  
Pattrarat Chanchaithong ◽  
Kittitat Lugsomya ◽  
Waree Niyomtham ◽  
Vanaporn Wuthiekanun ◽  
...  
Keyword(s):  
Rural Area ◽  
Molecular Detection ◽  
Domestic Animals ◽  
Water Sources ◽  
Pathogenic Leptospira

scholarly journals Molecular Detection of Acinetobacter Species in Lice and Keds of Domestic Animals in Oromia Regional State, Ethiopia

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52377 ◽  
Author(s):  
Bersissa Kumsa ◽  
Cristina Socolovschi ◽  
Philippe Parola ◽  
Jean-Marc Rolain ◽  
Didier Raoult
Keyword(s):  
Molecular Detection ◽  
Domestic Animals ◽  
Acinetobacter Species ◽  
Regional State

scholarly journals Molecular characterisation of Leptospira strains in Pakistan

2016 ◽  
Vol 60 (4) ◽  
pp. 417-421
Author(s):  
Muhammad Luqman Sohail ◽  
Muhammad Sarwar Khan ◽  
Muhammad Avais ◽  
Muhammad Yasir Zahoor ◽  
Irfan Khattak ◽  
...  
Keyword(s):  
16S Rrna ◽  
Animal Species ◽  
Molecular Detection ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Intermediate Species ◽  
Specific Primers ◽  
Pathogenic Leptospira ◽  
Wide Range ◽  
Serological Studies

Abstract Introduction: Leptospirosis affects a wide range of mammals, humans, and even a few poikilothermic animal species. In Pakistan, serological studies of equine leptospirosis have reported a prevalence of over 40%, but no study has ever been conducted towards molecular detection of Leptospira in horses. Material and Methods: Blood samples from 128 horses were screened using ELISA and 41 positive samples were examined for the presence of leptospiral DNA using specific primers for 16S rRNA gene. Results: Out of 41 tested samples, 20 samples were found to be PCR-positive, revealing a fragment of 306 bp after gel electrophoresis. Sequencing and phylogenetic analysis of positive samples revealed circulation of pathogenic Leptospira spp. in Pakistani horses. No evidence of circulation of intermediate species was found in this study. Conclusion: This study reports the first molecular evidence of equine leptospirosis in Pakistan and lays ground for further research in this area. It also confirms the efficiency of 16S rRNA for the diagnosis of equine leptospirosis.

scholarly journals Species composition and fauna distribution of mosquitoes (Diptera: Culicidae) and its importance for vector-borne diseases in a rural area of Central Western - Mato Grosso, Brazil

2017 ◽  
Vol 10 (2) ◽  
pp. 94-105 ◽  
Author(s):  
Fábio Alexandre Leal-Santos ◽  
Adaiane Catarina Marcondes Jacobina ◽  
Maria Madalena de Oliveira ◽  
Marinalva Brasilina Arruda Santana ◽  
Otacília Pereira Serra ◽  
...  
Keyword(s):  
Species Composition ◽  
Rural Area ◽  
Domestic Animals ◽  
The State ◽  
Tropical Diseases ◽  
Ecological Data ◽  
Mato Grosso ◽  
Vector Borne Diseases ◽  
The Family ◽  
Vector Borne

Abstract. This study describes ecological data obtained in a rural area in the State of Mato Grosso, including the insects belonging to the family Culicidae, especially those framed as potential vectors of tropical diseases. In 2015, we collected adult mosquitoes in fragments of forest in a rural area located in Mato Grosso Central West of Brazil. We captured 18,256 mosquitoes of the sub-families Culicinae and Anophelinae and have identified 34 species belonging to 12 genera: Aedes (1 species), Anopheles (8 species), Coquillettidia (1 species), Haemagogus (1 species), Culex (5 species), Psorophora  (5 species), Ochlerotatus (4 species), Deinocerites (1 species),  Mansonia (4 species), Sabethes (2 species), Limatus (1 species), Wyeomyia (1 species). The family Culicidae presented high richness and abundance, established by diversity indexes (Margalef α =3.26; Shannon H' = 2.09; Simpson D = 0.19) with dominance of the species Anopheles (Nyssorhyncus) darlingi Root (89.8%). This species has considerable epidemiological value, considered the main vector of malaria in Mato Grosso. Many species of mosquitoes are vectors of pathogens that cause disease in humans and domestic animals, transmitting pathogens including viruses (arboviruses), filaria worms (helminths) and protozoa.Composição de espécies e distribuição da fauna de mosquitos (Diptera: Culicidae) e sua importância para doenças transmitidas por vetores em uma área rural do centro-ocidental - Mato Grosso, BrasilResumo. Este estudo descreve dados ecológicos de uma área rural do Estado de Mato Grosso e dos insetos da família Culicidae especialmente aqueles enquadrados como vetores potenciais de doenças tropicais. Em 2015, coletamos mosquitos adultos em fragmentos de floresta em localidades de áreas rurais no Mato Grosso região Centro Oeste do Brasil. Foram capturados 18.256 exemplares alados de mosquitos das subfamílias Culicinae e Anophelinae e identificadas 34 espécies pertencentes a 12 gêneros: Aedes (1 espécie), Anopheles (8 espécies), Coquillettidia (1 espécie), Haemagogus (1 espécie), Culex (5 espécies), Psorophora (5 espécies), Ochlerotatus (4 espécies), Deinocerites (1 espécie), Mansonia (4 espécies), Sabethes (2 espécies), Limatus (1 espécie), Wyeomyia (1 espécie). A família Culicidae apresentou alta riqueza e abundância, estabelecida por índices de diversidade (Margalef α = 3.26, Shannon H' = 2.09, Simpson D = 0.19) com predominância da espécie Anopheles (Nyssorhyncus)darlingi Root (89.8%). Esta espécie tem considerável valor epidemiológico, sendo considerada o principal vetor de malária em Mato Grosso. Muitas espécies de mosquitos são vetores de patógenos que causam doenças em humanos e animais domésticos, transmitindo patógenos incluindo vírus (arboviroses), filárias (helmintos) e protozoários.

A Survey of Antibodies against Rickettsia rickettsii and Ehrlichia chafeensis in Domestic Animals from a Rural Area of Colombia

2009 ◽  
Vol 80 (6) ◽  
pp. 1029-1030 ◽  
Author(s):  
Marylin Hidalgo ◽  
Diana Lizarazo ◽  
Gustavo Valbuena ◽  
Juan Fernando Vesga
Keyword(s):  
Rural Area ◽  
Domestic Animals ◽  
Rickettsia Rickettsii

Molecular Detection ofToxoplasma gondiiin Water Sources of District Nowshehra, Khyber Pakhtunkhwa, Pakistan

2013 ◽  
Vol 76 (14) ◽  
pp. 837-841 ◽  
Author(s):  
Imran Khan ◽  
Amir Muhammad Khan ◽  
Sultan Ayaz ◽  
Sanaullah Khan ◽  
Muhammad Anees ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Water Sources ◽  
Khyber Pakhtunkhwa

The Research and Design of Horizontal Power Cutting Mixer

2012 ◽  
Vol 184-185 ◽  
pp. 574-577
Author(s):  
Xiang Xiong Zhao ◽  
Jiong Hui Liu
Keyword(s):  
Environmental Pollution ◽  
Rural Area ◽  
Domestic Animals ◽  
Comprehensive Utilization ◽  
Research And Design ◽  
Livestock Excreta ◽  
At Home

The environmental pollution of the rural area partly results from the excrement of sheep, cattle and poultry. In a systematic project of regulating and harnessing the excrement, the additive is applied to mix the faeces to remove the unbearable smell from livestock excreta and to make better and comprehensive utilization of the excreta. At present, the mixers at home and abroad are not applicable to mix the excrement of domestic animals for the reason that there are so many fibers in it. The shapes of sheep and cattle excrement like a ball which demands the rotor of the mixer to have the clipping function. Thus, the clipping and mixing machine is designed. Its principle, structure, technique and its main parameters are analyzed and proved in this paper.

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