Phase composition and morphological characterization of human kidney stones using IR spectroscopy, scanning electron microscopy and X-ray Rietveld analysis

Author(s):  
Paramita Chatterjee ◽  
Arup Chakraborty ◽  
Alok K. Mukherjee
2009 ◽  
Vol 42 (4) ◽  
pp. 629-635 ◽  
Author(s):  
Soumen Ghosh ◽  
Sharmila Basu ◽  
Santu Chakraborty ◽  
Alok K. Mukherjee

Structural and microstructural characterizations of eight human kidney stones (KS1–KS8) from eastern India have been carried out using IR spectroscopy, X-ray powder diffraction, scanning electron microscopy and thermogravimetric methods. An X-ray diffraction phase quantification revealed that three of the renal stones (KS1–KS3) were composed exclusively of calcium oxalate monohydrate (COM) and the remaining five (KS4–KS8) contained varying amounts of calcium oxalate dihydrate (40.1–53.0 wt%) and hydroxyapatite (1.3–17.3 wt%), in addition to the COM phase. The crystalline structure of COM (whewellite) at the atomic scale was redetermined through an X-ray powder diffraction study at room temperature using Rietveld analysis. Thermogravimetric analysis of KS1 reveals that COM (whewellite) is stable up to around 439 K, above which temperature anhydrous calcium oxalate is formed. The oxalate transforms to calcium carbonate at 751 K and finally to calcium oxide above 969 K. It should be emphasized that meaningful statistics in total number or gender specificity cannot be achieved with eight kidney stones.


Author(s):  
Samiran Pramanik ◽  
Soumen Ghosh ◽  
Arkaprovo Roy ◽  
Ramanuj Mukherjee ◽  
Alok Kumar Mukherjee

AbstractQuantitative phase composition and morphological characterization of 12 human gallbladder stones (GS1–GS12) retrieved from patients of eastern India have been carried out using IR-spectroscopy, powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM). The FTIR spectra indicated that the primary composition of gallstones studied was cholesterol. X-ray powder diffraction study revealed cholesterol monohydrate (CHM) as the major crystalline phase in GS1–GS12. The Rietveld analysis showed that nine of the gallstones were composed exclusively of CHM, while the remaining three stones contained in addition to CHM, small amounts (4.2–10.6 wt%) of calcium carbonate as aragonite and vaterite. The crystallite size of CHM in GS1–GS12 varied between 82(6) and 249(3) nm. The SEM images of gallstones showed different crystal habits of CHM such as plates, thin rods, rectangular and hexagonal blocks, which resulted into different levels of agglomeration at the mesoscopic scale. Presence of numerous parasitic eggs with a typical muskmelon surface in three gallstones (GS2, GS7 and GS9) suggests possible association between the liver fluke infection and biliary stone formation in these patients. To the best of our knowledge, the study constitutes the first report of X-ray quantitative phase analysis of gallstones using the Rietveld methodology.


Crystals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 654 ◽  
Author(s):  
Aleksei V. Rusakov ◽  
Mariya A. Kuzmina ◽  
Alina R. Izatulina ◽  
Olga V. Frank-Kamenetskaya

To study strontium (Sr) incorporation into calcium oxalates (weddellite and whewellite), calcium-strontium oxalate solid solutions (Ca,Sr)[C2O4]∙nH2O (n = 1, 2) are synthesized and studied by a complex of methods: powder X-ray diffraction (PXRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) spectroscopy. Two series of solid solutions, isomorphous (Ca,Sr)[C2O4]·(2.5 − x)H2O) (space group I4/m) and isodimorphous Ca[C2O4]·H2O(sp.gr. P21/c)–Sr[C2O4]·H2O(sp.gr. P 1 - ), are experimentally detected. The morphogenetic regularities of their crystallization are revealed. The factors controlling this process are discussed.


2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Liu Liu ◽  
Decai Gong ◽  
Zhengquan Yao ◽  
Liangjie Xu ◽  
Zhanyun Zhu ◽  
...  

Abstract Historically, sutras played an important role in spreading Buddhist faith and doctrine, and today these remain important records of Buddhist thought and culture. A Mahamayuri Vidyarajni Sutra with polychrome paintings was found inside the cavity on top of the Nanmen Buddhist pagoda, built in the early Tang dynasty (618–627 CE) and located in Anhui Province, China. Textile was found on the preface which is strongly degraded and fragile. Unfortunately, the whole sutra is under severe degradation and is incomplete. Technical analysis based on scientific methods will benefits the conservation of the sutra. Optical microscopy (OM), micro-Raman spectroscopy combined with optical microscope (Raman), scanning electron microscopy in combination with energy dispersive X-ray analysis (SEM–EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were used to characterize the pigment and gilded material, as well as the paper fiber and textile. Pigments such as cinnabar, minium, paratacamite, azurite, lead white were found. Gilded material was identified as gold. A five-heddle warp satin, made of silk, was found as the textile on the preface of the sutra. The sutra’s preface and inner pages were made of paper comprised of bamboo and bark. As a magnificent yet recondite treasure of Buddhism, the sutra was analyzed for a better understanding of the material. A conservation project of the sutra will be scheduled accordingly.


2012 ◽  
Vol 620 ◽  
pp. 314-319
Author(s):  
Nur Amira Mamat Razali ◽  
Fauziah Abdul Aziz ◽  
Saadah Abdul Rahman

Hardwood is wood from angiosperm trees. The characteristic of hardwood include flowers, endosperm within seeds and the production of fruits that contain the seeds. This paper aims to discuss the preparation and characterization of cellulose obtained from hardwood. The hardwood Merbau (Intsia bijuga) was chosen as raw material in this study. Alkaline treatment and delignification methods were used for the preparation of cellulose. Acid hydrolysis was employed to produce cellulose nanocrystal (CNC). The treated and untreated samples were characterized using x-ray diffraction (XRD) and field emission scanning electron microscopy (FE-SEM). The final product, from both trated and untreated samples were then compared.


2012 ◽  
Vol 174-177 ◽  
pp. 508-511
Author(s):  
Lin Lin Yang ◽  
Yong Gang Wang ◽  
Yu Jiang Wang ◽  
Xiao Feng Wang

BiFeO3 polyhedrons had been successfully synthesized via a hydrothermal method. The as-prepared products were characterized by power X-ray diffraction (XRD) pattern, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The possible mechanisms for the formation of BiFeO3 polyhedrons were discussed. Though comparison experiments, it was found that the kind of precursor played a key role on the morphology control of BiFeO3 crystals.


2022 ◽  
Vol 321 ◽  
pp. 126326
Author(s):  
Gladis Aparecida Galindo Reisemberger de Souza ◽  
Ramón Sigifredo Cortés Paredes ◽  
Frieda Saicla Barros ◽  
Gustavo Bavaresco Sucharski ◽  
Sebastião Ribeiro Junior ◽  
...  

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