The control of the adsorption of bovine serum albumin on mercaptan-modified gold thin films investigated by SERS spectroscopy

2018 ◽  
Vol 204 ◽  
pp. 119-124 ◽  
Author(s):  
Lucas de Oliveira Noman ◽  
Antonio Carlos Sant'Ana
Keyword(s):  
Thin Films ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gold Thin Films

Poly(allylamine hydrochloride) (PAH) and Bovine Serum Albumin (BSA) Protein Nanostructured as Layer-by-Layer Thin Films

2018 ◽  
Vol 18 (6) ◽  
pp. 3908-3915 ◽  
Author(s):  
Antonio Wellington Lima de Sa ◽  
Hugo Gajardoni de Lemos ◽  
Marcelo A Pereira-da-Silva ◽  
Everaldo Carlos Venancio
Keyword(s):  
Thin Films ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Layer By Layer ◽  
Allylamine Hydrochloride

Optical Properties of Thin Layers of Bovine Serum Albumin, γ-Globulin, and Hemoglobin*

1986 ◽  
Vol 40 (3) ◽  
pp. 313-318 ◽  
Author(s):  
H. Arwin
Keyword(s):  
Thin Films ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Dielectric Response ◽  
Thin Layers ◽  
Silicon Substrates ◽  
Protein Films ◽  
Effective Medium Theories ◽  
Monomolecular Films

We have used spectroscopic ellipsometry to determine the dielectric functions of thin films of γ-globulin, bovine serum albumin, and hemoglobin in the visible and near-uv photon energy range. We show that both thickness and dielectric function can be resolved for monomolecular films adsorbed on substrates with relatively low polarizability. The data which we consider to be closest to the intrinsic dielectric response of the protein films were obtained on HgTe and HgCdTe substrates. Less resolution was obtained on silicon substrates. For a density-deficient film, we were able to model the dielectric response with effective medium theories, and the void fraction could be determined.

Pulsed laser deposition of bovine serum albumin protein thin films

2003 ◽  
Vol 208-209 ◽  
pp. 658-662 ◽  
Author(s):  
M.A. Hernandez-Perez ◽  
C. Garapon ◽  
C. Champeaux ◽  
P. Shahgaldian ◽  
A. Coleman ◽  
...  
Keyword(s):  
Thin Films ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Pulsed Laser Deposition ◽  
Bovine Serum ◽  
Pulsed Laser ◽  
Laser Deposition ◽  
Bovine Serum Albumin Protein

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

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