Antioxidant activities of aqueous and ethyl acetate fractions of Daucus carota L. seed against triton X-100 induced oxidative stress in mice

Free radicals are chemical species with unpaired electrons in their outer orbital that can attack other molecules, causing cell oxidative damage and degenerative diseases. Free radicals can be prevented by antioxidant. An antioxidant can be found in nature as secondary metabolites in plants, such as carrot (Daucus carota L.). This research was conducted to study the antioxidant activities of roots, leaves, and stems of carrot extracts using DPPH and FRAP methods, determine total phenolic content (TPC) and total flavonoid content (TFC), analyse the correlation between TPC and TFC with AAI DPPH and FRAP, and the correlation between two methods. The sample was extracted by reflux using n-hexane, ethyl acetate, and ethanol. Determination of TPC, TFC, AAI DPPH and FRAP was performed using UV-visible spectrophotometry. Correlation of TPC and TFC with AAI DPPH and FRAP and also the correlation between the two methods were conducted using Pearson's method. Ethyl acetate carrot leaves extract showed the highest TPC and TFC (8.88 ± 0.44 g GAE/100 g and 9.00 ± 0.31 g QE/100 g). AAI DPPH of carrot extract in the range of 0.16 – 1.42, meanwhile AAI FRAP 1.89 – 5.45. TPC and TFC of carrot roots extract showed a significantly positive correlation with AAI DPPH and FRAP. AAI DPPH and FRAP of carrot roots extract gave a significantly positive correlation. Ethyl acetate and ethanol carrot leave extracts were strong to very strong antioxidant by DPPH and FRAP methods. TPC and TFC in carrot roots extract contributed to antioxidant activities by DPPH and FRAP. DPPH and FRAP presented linear results in antioxidant activities of carrot roots extract.

Download Full-text

Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L.

Archives of Biological Sciences ◽

10.2298/abs160902123h ◽

2017 ◽

Vol 69 (3) ◽

pp. 481-489

Author(s):

Haote Han ◽

Na Liu ◽

Lin Zhang ◽

Minghua Gong ◽

Ming Cao ◽

...

Keyword(s):

Oxidative Stress ◽

Prostate Cancer ◽

Cell Death ◽

Daucus Carota ◽

Daucus Carota L ◽

Cytoplasmic Vacuolation ◽

Cell Death Pathway ◽

Hela Cell Lines

We investigated the antitumor activity of daucusol (DS) derived from Daucus carota L. in PC-3, A549 and HeLa cell lines by the MTT assay. Optical microscopy revealed that exposure of PC-3 cells to DS resulted in cytoplasmic vacuolation. Flow cytometry analysis of the phase of the cell cycle did not reveal a sub-G1 peak, and no caspase-dependent activation was observed after DS treatment. The levels of endoplasmic reticulum (ER) stress biomarkers, LC3B-II and ubiquitinated proteins were increased. It was also observed that oxidative stress played an important role in the activation of the cytoplasmic vacuolation-mediated cell-death pathway. In vivo, DS inhibited tumor growth in nude mice by 39.13% compared to the vehicle. Protein expression in the tumor tissue was consistent with their expression in vitro. Our findings indicate that DS induced cytoplasmic vacuolation-mediated death in PC-3 cells by triggering oxidative stress and suggest that targeting this pathway could serve as a novel therapeutic approach for prostate cancer.

Download Full-text

DAYA TERIMA JAJANAN LOKAL BARONCONG DENGAN PENAMBAHAN WORTEL (DAUCUS CAROTA L)

Media Gizi Pangan ◽

10.32382/mgp.v26i1.993 ◽

2019 ◽

Vol 26 (1) ◽

pp. 1

Author(s):

Suriani Rauf ◽

Manjilala Manjilala ◽

Elvi Kusuma

Keyword(s):

Experimental Study ◽

Daucus Carota ◽

Vegetable Consumption ◽

Negative Impact ◽

Local Foods ◽

Ministry Of Health ◽

Daucus Carota L

The behavior of people who less consume vegetables and fruit can have a negative impact on health. The lack of appetite for vegetable consumption by the society made the researchers interested in making a combination of vegetables, namely carrots with local foods, namely Baroncong.This research aims to determine the acceptability of local baroncong snacks with the addition of carrots.This research is a pre-experimental study. The panelists from this study consisted of 30 Nutrition Department Students from Health Polytechnic Ministry of Health Makassar. Assessment based on taste aspects, color of aroma and texture of baroncong products with the addition of carrots 50%, 75%, 100% with the criteria of very like, like, dislike and very dislike.The results showed that the acceptability of baroncong with the addition of carrots in terms of taste was a concentration of 75%, which was very much as much as 46.7% and liked as much as 50%, the color aspect was 50% concentration consisting of 36.7% likes and likes as much as 53.3%, the aroma aspectis a concentration of 50% which consists of very much as much as 33.3% and likes as much as 63.3%, in terms of aspects of texture are concentrations of 50% and 75%, each of which consists of as much as 23.3% and likes as much as 63, 3%. Based on these data, Baroncong with the addition of carrots received is a concentration of 75%.

Download Full-text