Actigard™ induces a defence response to limit Pseudomonas syringae pv. actinidiae in Actinidia chinensis var. chinensis ‘Hort16A’ tissue culture plants.

2022 ◽  
Vol 295 ◽  
pp. 110806
Author(s):  
Erin.A. Stroud ◽  
Erik.H.A. Rikkerink ◽  
Jay Jayaraman ◽  
Matthew D. Templeton
Keyword(s):  
Tissue Culture ◽  
Pseudomonas Syringae ◽  
Defence Response ◽  
Actinidia Chinensis

scholarly journals Draft Genome Resources Sequences of Six Pseudomonas syringae pv. actinidiae Strains Isolated from Actinidia chinensis var. deliciosa Leaves in Portugal

2020 ◽  
pp. PHYTO-05-20-018
Author(s):  
Aitana Ares ◽  
Marta Tacão ◽  
Daniela Figueira ◽  
Eva Garcia ◽  
Joana Costa
Keyword(s):  
Genetic Diversity ◽  
Pseudomonas Syringae ◽  
Draft Genome ◽  
Clonal Expansion ◽  
Average Nucleotide Identity ◽  
Actinidia Chinensis ◽  
Valuable Resource ◽  
Genetic Population ◽  
Evolutionary Studies ◽  
Reference Strains

Pseudomonas syringae pv. actinidiae is a quarantine bacterium affecting all the Portuguese main areas of kiwifruit production. We report the draft genome of six P. syringae pv. actinidiae strains isolated from symptomatic leaves of Actinidia chinensis var. deliciosa in a study that determined the genetic population structure of the endophytic and epiphytic populations in two consecutive seasons. Average nucleotide identity values were above 99% similarity with reference strains from P. syringae pv. actinidiae biovar 3. The genomic differences found between these strains confirm the genetic diversity described for P. syringae pv. actinidiae population in Portugal. Furthermore, data provide evidence that the initial clonal expansion of P. syringae pv. actinidiae in Europe was followed by a genomic diversification constituting a valuable resource for epidemiological and evolutionary studies, namely when adopting strategies for epidemics management.

Proteomic changes in Actinidia chinensis shoot during systemic infection with a pandemic Pseudomonas syringae pv. actinidiae strain

2013 ◽  
Vol 78 ◽  
pp. 461-476 ◽  
Author(s):  
Milena Petriccione ◽  
Ilaria Di Cecco ◽  
Simona Arena ◽  
Andrea Scaloni ◽  
Marco Scortichini
Keyword(s):  
Pseudomonas Syringae ◽  
Systemic Infection ◽  
Actinidia Chinensis

scholarly journals Infection of ZESPRI GOLD kiwifruit (Actinidia chinensis Hort16A) lenticels by Pseudomonas syringae pv actinidiae

2012 ◽  
Vol 65 ◽  
pp. 289-289
Author(s):  
N.J. Larsen ◽  
P.W. Sutherland ◽  
I.C. Hallett ◽  
M.K. Jones ◽  
I.P.S. Pushparajah ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Pseudomonas Syringae ◽  
Actinidia Chinensis ◽  
Gaseous Exchange ◽  
Woody Tissue ◽  
Severe Infections

Pseudomonas syringae pv actinidiae (Psa) is a bacterium whose virulent form (PsaV) causes severe infections of kiwifruit particularly Actinidia chinensis Hort16A Lenticels on kiwifruit canes function as pores allowing gaseous exchange The lenticellular structure penetrates through the periderm potentially allowing bacterial entry and subsequent cortex infection Bacteria have been observed inside and directly below lenticels from ca 3yearold woody tissue from the field To investigate this pathway of bacterial infection further lenticels from three wood ages were inoculated with a strain of PsaV at 109 cfu/ml Brown staining was observed on lenticels 3 days after inoculation on the youngest wood (< 1 year old) Lenticels were sectioned 2 and 4 weeks after inoculation and isolations were conducted from sterilised tissue after 4 weeks Psa was visible inside lenticels of the youngest wood 2 weeks postinoculation and was also isolated from the youngest tissue after 4 weeks Bacteria were not observed in lenticels of older wood and Psa was not isolated

scholarly journals Pseudomonas syringae pv. actinidiae survival in point-inoculated kiwifruit vines

2018 ◽  
Vol 71 ◽  
pp. 45-50
Author(s):  
Joy L. Tyson ◽  
Michael A. Manning ◽  
Kieran D. Mellow ◽  
Michelle J. Vergara
Keyword(s):  
Pseudomonas Syringae ◽  
Winter Period ◽  
Actinidia Chinensis ◽  
Active Growth ◽  
Over Time ◽  
Apparently Healthy

The survival and spread over time of Pseudomonas syringae pv. actinidiae (Psa) in point-inoculated kiwifruit vines is poorly understood. Forty-eight 2-year-old vines of Actinidia chinensis var. deliciosa ‘Hayward’ and A. chinensis var. chinensis ‘Hort16A’ were inoculated 30 cm above the crown, either during the active growth (autumn) or dormant  (winter) period in two successive years. Vines were cultivated for 3—4 years, after which bacterial isolations were made at intervals along the vines from crown to tip. Psa was found up to 220 cm above the inoculation point and in some of the crowns, 30 cm below the inoculation point. The sites where Psa was found within vines were not always contiguous. Fewer vines of ‘Hayward’ developed serious symptoms or died than ‘Hort16A’; however, more surviving vines of ‘Hayward’ were Psa-positive than those of ‘Hort16A’. Psa was able to survive for at least 4 years in apparently healthy kiwifruit vines. This has implications for the movement of asymptomatic budwood to areas without Psa.

scholarly journals Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1350
Author(s):  
Tao Wang ◽  
Zhan-Hui Jia ◽  
Ji-Yu Zhang ◽  
Min Liu ◽  
Zhong-Ren Guo ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Plant Immunity ◽  
Domain Architecture ◽  
Bacterial Canker ◽  
Actinidia Chinensis ◽  
R Genes ◽  
Leucine Rich Repeat ◽  
Cis Acting ◽  
Important Disease

Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the present study, we identified 100 NBS-LRR genes in the kiwifruit genome and they were grouped into six distinct classes based on their domain architecture. Of the 100 genes, 79 are truncated non-regular NBS-LRR genes. Except for 37 NBS-LRR genes with no location information, the remaining 63 genes are distributed unevenly across 18 kiwifruit chromosomes and 38.01% of them are present in clusters. Seventeen families of cis-acting elements were identified in the promoters of the NBS-LRR genes, including AP2, NAC, ERF and MYB. Pseudomonas syringae pv. actinidiae (pathogen of the kiwifruit bacterial canker) infection induced differential expressions of 16 detected NBS-LRR genes and three of them are involved in plant immunity responses. Our study provides insight of the NBS-LRR genes in kiwifruit and a resource for the identification of new R-genes in the fruit.

scholarly journals Rapid Evaluation of Pathogenicity in Pseudomonas syringae pv. syringae with a Lilac Tissue Culture Bioassay and Syringomycin DNA Probes

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 905-910 ◽  
Author(s):  
Heather J. Scheck ◽  
Marilyn L. Canfield ◽  
Jay W. Pscheidt ◽  
Larry W. Moore
Keyword(s):  
Tissue Culture ◽  
Pacific Northwest ◽  
Pseudomonas Syringae ◽  
Woody Plants ◽  
Dna Probes ◽  
Rapid Evaluation ◽  
Colony Hybridization ◽  
The Pacific ◽  
One Step ◽  
Tissue Culture Plantlets

Losses from diseases caused by Pseudomonas syringae pv. syringae occur on a large number of deciduous woody plants in commercial nurseries in the Pacific Northwest. Bioassays for pathogenicity are one step in the identification of P. syringae pv. syringae and are usually performed on the host of isolation; however, woody plants can take months to develop symptoms. A bioassay with highly susceptible lilac (Syringa vulgaris ‘Sensation’) tissue culture plantlets evaluated pathogenicity in strains of P. syringae pv. syringae isolated from 25 species of deciduous woody plants. DNA colony hybridization with the syrB probe for a syringomycin synthetase gene and the syrD probe for a syringomycin export gene was also evaluated as a method for identifying pathogens. Of 552 strains provisionally identified as P. syringae pv. syringae, 59% were pathogenic in the bioassay and hybridized with the syr probes, while 19% were non-pathogenic and did not hybridize with the syr probes, giving 78% agreement between the two methods. Nine percent of strains were pathogenic in the bioassay but did not hybridize with the syr probes, and 13% were not pathogenic in the bioassay but did hybridize with the syr probes. These methods detected pathogenic strains of P. syringae pv. syringae isolated from diverse woody plants in 5 to 16 days.

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