Morphology and genic-SSRs-based diversity analysis and georeferencing of economic traits in natural populations of Jack (Artocarpus heterophyllus Lam.) from Eastern India

Jackfruit (Artocarpus heterophyllus Lam.) being an important dryland horticultural tree, grows well with minimum care and maintenance. The study aimed at identifying the genetic diversity present in twenty jackfruit genotypes for different pulp colours. The genotypes were identified for commercial cultivation, based on fruit and flake quality characters viz., fruit weight, fruit rind weight per kg fruit, number of flakes per kg fruit, weight of flakes per kg fruit, individual fresh flake weight and TSS as per the Jackfruit descriptors given by IPGRI, Rome. The best genotypes identified from the study were Swarna, Lalbagh Madhura, Byrachandra, NSP, Ashoka Yellow and NEL and these can be used for commercial purpose. Molecular diversity analysis was also carried out using 22 SSR primers, out of which six primers (SSR 9, SSR 10, SSR 30, SSR 34, SSR 45 and SSR 48) showed polymorphism among twenty genotypes. The genetic similarity co-efficient ranged from 0 to 0.96, indicating a vast variation in genetic diversity for pulp colour. A dendrogram was constructed by UPGMA analysis revealed three major clusters. The genotypes with cream and yellow colour pulp grouped in cluster I and II, followed by yellow, orange and red in cluster III. These markers effectively segregated the genotypes based on different pulp colours and hence, can be used for both diversity analysis and in breeding applications.

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Evaluation of genetic polymorphism of proteins in stellate sturgeon Acipenser stellatus (Pall) in aquaculture with reference to economically valuable traits

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/937/3/032033 ◽

2021 ◽

Vol 937 (3) ◽

pp. 032033

Author(s):

Elena Shishanova ◽

Grigory Shishanov ◽

Dmitry Isaev

Keyword(s):

Genetic Polymorphism ◽

Survival Rates ◽

Natural Populations ◽

Natural Environments ◽

Economic Traits ◽

Allelic Variants ◽

Stellate Sturgeon ◽

Size Growth ◽

Economically Valuable Traits ◽

Acipenser Stellatus

Abstract One of the main goals of sturgeon aquaculture is the restoration and renewal of wild populations of sturgeon fish in nature, which creates a tension between selective breeding with maintenance of valuable economic traits in a homozygous state and the need to conserve genetic diversity ensuring fitness in natural environments. It is generally assumed that important economic characteristics, such as body weight and size, growth rate, fecundity, etc., as well as fitness in fish, are associated with heterozygosity and genetic polymorphism of some key metabolic enzymes. It remains unclear whether aquaculture conditions can be as a whole selection factor in favor of certain allelic variants of these enzymes. To establish the relationship between some economic traits and enzymatic polymorphism, we studied the distribution of allelic variants of LDH-3, AAT-2, FGM, and Est enzymes in stellate sturgeon reared in aquaculture. We revealed a frequency bias of some allelic variants of the studied enzymes in a cohort of fish from the generation bred in aquaculture compared to the frequencies of those alleles in wild natural populations. Our study suggests that industrial breeding promotes selection in the direction of reducing size but increasing survival rates, which correlates with certain allelic variants.

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Identification of Genic SSRs Provide a Perspective for Studying Environmental Adaptation in the Endemic Shrub Tetraena mongolica

Genes ◽

10.3390/genes11030322 ◽

2020 ◽

Vol 11 (3) ◽

pp. 322 ◽

Cited By ~ 1

Author(s):

Zhenhua Dang ◽

Lei Huang ◽

Yuanyuan Jia ◽

Peter J. Lockhart ◽

Yang Fong ◽

...

Keyword(s):

Natural Populations ◽

Environmental Adaptation ◽

Survival Strategies ◽

Kegg Pathways ◽

Polymorphic Ssrs ◽

Genic Ssrs ◽

Heterogeneous Habitats ◽

Fast Adaptation ◽

Go Terms ◽

Simple Sequence

Tetraena mongolica is a xerophytic shrub endemic to desert regions in Inner Mongolia. This species has evolved distinct survival strategies that allow it to adapt to hyper-drought and heterogeneous habitats. Simple sequence repeats (SSRs) may provide a molecular basis in plants for fast adaptation to environmental change. Thus, identifying SSRs and their possible effects on gene behavior has the potential to provide valuable information for studies of adaptation. In this study, we sequenced six individual transcriptomes of T. mongolica from heterogeneous habitats, focused on SSRs located in genes, and identified 811 polymorphic SSRs. Of the identified SSRs, 172, 470, and 76 were located in 5′ UTRs, CDSs, and 3′ UTRs in 591 transcripts; and AG/CT, AAC/GTT, and AT/AT were the most abundant repeats in each gene region. Functional annotation showed that many of the identified polymorphic SSRs were in genes that were enriched in several GO terms and KEGG pathways, suggesting the functional significance of these genes in the environmental adaptation process. The identification of polymorphic genic SSRs in our study lays a foundation for future studies investigating the contribution of SSRs to regulation of genes in natural populations of T. mongolica and their importance for adaptive evolution of this species.

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