A comprehensive insight into the application of white rot fungi and their lignocellulolytic enzymes in the removal of organic pollutants

2021 ◽  
Vol 778 ◽  
pp. 146132
Author(s):  
Rui Zhuo ◽  
Fangfang Fan
Keyword(s):  
Organic Pollutants ◽  
White Rot Fungi ◽  
White Rot ◽  
Lignocellulolytic Enzymes ◽  
Insight Into

Mycoremediation of Lignocelluloses

Biotechnology ◽  
2019 ◽  
pp. 1086-1108
Author(s):  
Saritha Vara
Keyword(s):  
Spatial Distribution ◽  
Animal Feed ◽  
Space Exploration ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Mechanisms Of Action ◽  
White Rot ◽  
Lignocellulolytic Enzymes ◽  
Biotechnological Applications ◽  
Potential Use

The most abundant aromatic biopolymer on earth Lignin is extremely recalcitrant to degradation. It creates a barrier to solutions or enzymes by linking to both hemicellulose and cellulose preventing the penetration of lignocellulolytic enzymes into the interior lignocellulosic structure. Global attention has been gained by fungi owing to the potential use of their versatile enzymes for agriculture, medicines, industries and bioremediation. The combination of extracellular ligninolytic enzymes, mediators, organic acids and accessory enzymes make some of the basidiomycete white-rot fungi to be able to degrade lignin efficiently. This review describes remediation of lignocelluloses by fungi, properties of fungi, their spatial distribution and the mechanisms of action which render them attractive candidates in biotechnological applications like biopulping, animal feed, genetic engineering and space exploration.

Mycoremediation of Lignocelluloses

2017 ◽  
pp. 264-286 ◽  
Author(s):  
Saritha Vara
Keyword(s):  
Spatial Distribution ◽  
Animal Feed ◽  
Space Exploration ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Mechanisms Of Action ◽  
White Rot ◽  
Lignocellulolytic Enzymes ◽  
Biotechnological Applications ◽  
Potential Use

The most abundant aromatic biopolymer on earth Lignin is extremely recalcitrant to degradation. It creates a barrier to solutions or enzymes by linking to both hemicellulose and cellulose preventing the penetration of lignocellulolytic enzymes into the interior lignocellulosic structure. Global attention has been gained by fungi owing to the potential use of their versatile enzymes for agriculture, medicines, industries and bioremediation. The combination of extracellular ligninolytic enzymes, mediators, organic acids and accessory enzymes make some of the basidiomycete white-rot fungi to be able to degrade lignin efficiently. This review describes remediation of lignocelluloses by fungi, properties of fungi, their spatial distribution and the mechanisms of action which render them attractive candidates in biotechnological applications like biopulping, animal feed, genetic engineering and space exploration.

scholarly journals Late-Stage Functionalisation of Polycyclic (N-Hetero-) Aromatic Hydrocarbons by Detoxifying CYP5035S7 Monooxygenase of the White-Rot Fungus Polyporus arcularius

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1708
Author(s):  
Nico D. Fessner ◽  
Christopher Grimm ◽  
Wolfgang Kroutil ◽  
Anton Glieder
Keyword(s):  
Aromatic Hydrocarbons ◽  
Active Site ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Cytochrome P450 Enzymes ◽  
Factors Influencing ◽  
Steric Factors ◽  
Polycyclic Aromatic ◽  
Insight Into

Functionalisation of polycyclic aromatic hydrocarbons (PAHs) and their N-heteroarene analogues (NPAHs) is a tedious synthetic endeavour that requires diverse bottom-up approaches. Cytochrome P450 enzymes of white-rot fungi were shown to participate in the fungal detoxification of xenobiotics and environmental hazards via hydroxylation of PAH compounds. In this paper, the recently discovered activity of the monooxygenase CYP5035S7 towards (N)PAHs was investigated in detail, and products formed from the substrates azulene, acenaphthene, fluorene, anthracene, and phenanthrene by whole-cell biocatalysis were isolated and characterised. The observed regioselectivity of CYP5035S7 could be explained by a combination of the substrate’s electron density and steric factors influencing the substrate orientation giving insight into the active-site geometry of the enzyme.

Characterization of Lignocellulolytic Enzymes from White-Rot Fungi

2014 ◽  
Vol 70 (4) ◽  
pp. 485-498 ◽  
Author(s):  
Tamilvendan Manavalan ◽  
Arulmani Manavalan ◽  
Klaus Heese
Keyword(s):  
White Rot Fungi ◽  
White Rot ◽  
Lignocellulolytic Enzymes

scholarly journals Coupling structural characterization with secretomic analysis reveals mechanism of the disruption of cross-linked structure in bamboo culms by Echinodontium taxodii

2019 ◽  
Author(s):  
Wen Kong ◽  
Jialong Zhang ◽  
Qiuyun Xiao ◽  
Jiashu Liu ◽  
Zhixiang Cao ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Extracellular Enzymes ◽  
Early Stage ◽  
White Rot Fungi ◽  
White Rot ◽  
Biomass Recalcitrance ◽  
Lignocellulose Conversion ◽  
Fungal Pretreatment ◽  
Insight Into

Abstract Background: Overcoming the biomass recalcitrance is essential for efficient utilization of lignocellulosic biomass in industrial bio-refining. White-rot fungi can overcome the biomass recalcitrance and accelerate the conversion of lignocellulose to biofuels via a large number of special extracellular lignocellulolytic enzymes. Previous studies try to dissect the function of extracellular enzymes on biomass resistant cross-linked structures by secretome analysis, but the bio-alteration of cross-linked structures is ignored usually. A deeper and detailed understanding of relationship between secretome and bio-alteration of cross-linked structure in lignocellulosic biomass is still lack. Results: As an efficient wood-decaying fungus, Echinodontium taxodii could improve the conversion efficiency of lignocellulose to biofuels. This study coupled comparative analysis of fungal secretomes and 2D HSQC NMR analysis of lignocellulose fractions, aiming to elucidate the role of extracellular enzymes from Echinodontium taxodii 2538 in the disruption of resistant cross-linked structure of bamboo culms. Carboxylesterases, alcohol oxidases and Class-II peroxidases showed importance in the cleavage of cross-linked structures, including ester and ether linkages of lignin-carbohydrate complexes (LCCs) and inter-unit linkages of lignin, which contributed to biomass resistance removal and cellulose exposure during the early stage of fungal decay. Moreover, the rapid oxidation of Cα-OH was found to contribute to the lignin bio-depolymerization. Conclusions: These findings revealed the detailed mechanisms of biomass recalcitrance reduction by fungal pretreatment, and provide insight into efficient strategy of lignocellulose conversion. It will advance the development in design of enzyme cocktail for efficient lignocellulose bio-refinery.

Ligninolytic Activity of White Rot Fungi from GGV campus as Bioremediation of Organic Pollutants

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Sushil Kumar Shahi ◽  
Nikki Agrawal
Keyword(s):  
Organic Pollutants ◽  
Organic Pollutant ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Ligninolytic Activity ◽  
The North ◽  
Physiological Group ◽  
Wide Range

White rot fungi constitute a diverse physiological group are capable of transforming and mineralizing a wide range of organopollutants. The Ligninolytic enzymes of white rot fungi are substrate specific, essential for Lignin degradation and organic pollutant remediation. In the present study white rot fungi were collected from the north region of Chhattisgarh of Guru Ghasidas Vishwavidyalaya (GGV) Campus. 40 species were collected and isolated then Qualitative and Quantitative screening for the Ligninolytic enzyme assaywere carried out. Out of 40 species, 5 species show potent Ligninolytic activity. In future we can utilize these fungi for the degradation of organic pollutants.

OPTIMIZATION OF BIOMASS AND ENDO-B-1,4-GLUCANASE BY WHITE ROT FUNGI NATIVE FROM ARGENTINA

2017 ◽  
Vol 16 (11) ◽  
pp. 2581-2588
Author(s):  
Ernesto M. Giorgio ◽  
Maria I. Fonseca ◽  
Andrea L. Morales ◽  
Pedro D. Zapata ◽  
Laura L. Villalba
Keyword(s):  
White Rot Fungi ◽  
White Rot
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