Suspect screening of antimicrobial agents transformation products in environmental samples development of LC-QTrap method running in pseudo MRM transitions

Twenty-one isolates of Listeria monocytogenes from cabbage, environmental, and water samples were evaluated for antimicrobial resistance by the disk diffusion method. Ninety-five percent (20 of 21) of the isolates tested were resistant to two or more antimicrobial agents. This finding is significant, since multiresistant strains of Listeria spp. are not commonly found in nature. Eighty-five percent (17 of 20) of the multiresistant strains were resistant to penicillin, and the remaining multiresistant isolates were somewhat sensitive to penicillin. A multiresistant strain showing intermediate sensitivity to penicillin was resistant to gentamicin. One isolate was susceptible to all antimicrobial agents except penicillin. Penicillin- and gentamicin-resistant L. monocytogenes have not previously been reported from human, food, or environmental samples. This study provides evidence of the emergence of multiresistant L. monocytogenes strains, pointing to an increase in the potential threat to human health posed by this pathogen.

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Quantifying summed fullerene nC60 and related transformation products in water using LC LTQ Orbitrap MS and application to environmental samples

Environment International ◽

10.1016/j.envint.2011.03.020 ◽

2011 ◽

Vol 37 (6) ◽

pp. 1063-1067 ◽

Cited By ~ 33

Author(s):

Annemarie P. van Wezel ◽

Véronique Morinière ◽

Erik Emke ◽

Thomas ter Laak ◽

Ariadne C. Hogenboom

Keyword(s):

Environmental Samples ◽

Transformation Products ◽

Orbitrap Ms

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Beating the Bio-Terror Threat with Rapid Antimicrobial Susceptibility Testing

Microorganisms ◽

10.3390/microorganisms9071535 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1535

Author(s):

Shahar Rotem ◽

Ida Steinberger-Levy ◽

Ofir Israeli ◽

Eran Zahavy ◽

Ronit Aloni-Grinstein

Keyword(s):

Antibiotic Susceptibility ◽

Environmental Samples ◽

Antimicrobial Agents ◽

Susceptibility Testing ◽

Development Stage ◽

Post Exposure Prophylaxis ◽

Bacterial Agent ◽

Susceptibility Tests ◽

Exposure Prophylaxis ◽

And Control

A bioterror event using an infectious bacterium may lead to catastrophic outcomes involving morbidity and mortality as well as social and psychological stress. Moreover, a bioterror event using an antibiotic resistance engineered bacterial agent may raise additional concerns. Thus, preparedness is essential to preclude and control the dissemination of the bacterial agent as well as to appropriately and promptly treat potentially exposed individuals or patients. Rates of morbidity, death, and social anxiety can be drastically reduced if the rapid delivery of antimicrobial agents for post-exposure prophylaxis and treatment is initiated as soon as possible. Availability of rapid antibiotic susceptibility tests that may provide key recommendations to targeted antibiotic treatment is mandatory, yet, such tests are only at the development stage. In this review, we describe the recently published rapid antibiotic susceptibility tests implemented on bioterror bacterial agents and discuss their assimilation in clinical and environmental samples.

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