Effect of the supplementation of fish oil on the conjugated linoleic acid production, especially accumulation of trans-11 C18:1 and trans-10 C18:1 fatty acids: An in vitro rumen fermentation study

Purpose: To investigate the effect of dietary supplementation of two omega fatty acids on in vitro rumen fermentation, microbial populations, total gas and methane (CH4) production．Methods: Both linoleic and linolenic acids were supplemented at 0 (control), 1, 3, 5 and 7 % of dry matter (DM) in a ration with a high roughage to concentrate ratio (70: 30). Total gas and CH4 were measured at 3, 6, 9, 12 and 24 h of fermentation while pH, volatile fatty acids (VFA), and ammonia nitrogen (NH3-N) concentrations were measured at 24 h using buffalo rumen fluid in an in vitro batch culture system. Microbial populations were determined using 16S-rDNA gene primers by RT-PCR.Results: The results revealed that linoleic acid at 3, 5 and 7 % decreased the concentration of NH3-N (p< 0.05) but linolenic acid at 5 and 7 % increased NH3-N (p < 0.05). A linear decrease (p <0.001) in acetate and butyrate, coupled with linear increase (p <0.001) in propionate was observed in response to treatment. Furthermore, supplementation of 3, 5 and 7 % of both fatty acids linearly (p < 0.001) decreased total gas and CH4 production when compared to the control. The addition of linoleic acid linearly (p < 0.001) decreased the number of protozoa without affecting methanogens, while linolenic acid linearly and quadratically (p < 0.001) reduced the population of both protozoa and methanogens (p < 0.05).Conclusion: Linolenic acid is more effective at a 3 % level in reducing methane production (up to 63 %) in high roughage diets.

Download Full-text

Effect of supplementing conjugated linoleic acid producing Bifidobacterial strains on in vitro rumen fermentation attributes

Indian Journal of Animal Nutrition ◽

10.5958/2231-6744.2020.00022.5 ◽

2020 ◽

Vol 37 (2) ◽

pp. 132

Author(s):

Neeru Jaglan ◽

Sachin Kumar ◽

Prasanta Kumar Choudhury ◽

Bhawna Tyagi ◽

P.S. Banakar ◽

...

Keyword(s):

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Rumen Fermentation ◽

In Vitro Rumen Fermentation

Download Full-text

Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽

10.3390/ani11010108 ◽

2021 ◽

Vol 11 (1) ◽

pp. 108

Author(s):

Yichong Wang ◽

Sijiong Yu ◽

Yang Li ◽

Shuang Zhang ◽

Xiaolong Qi ◽

...

Keyword(s):

Fatty Acids ◽

Acetic Acid ◽

Propionic Acid ◽

Acid Content ◽

Volatile Fatty Acids ◽

Rumen Fermentation ◽

Gas Production ◽

Quadratic Response ◽

In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

Download Full-text