Planar poly(dimethylsiloxane) (PDMS)–glass hybrid microdevice for a flow-through polymerase chain reaction (PCR) employing a single heater assisted by an intermediate metal alloy layer for temperature gradient formation

2014 ◽  
Vol 190 ◽  
pp. 177-184 ◽  
Author(s):  
Kieu The Loan Trinh ◽  
Wenming Wu ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Temperature Gradient ◽  
Alloy Layer ◽  
Metal Alloy ◽  
Chain Reaction ◽  
Gradient Formation ◽  
Flow Through ◽  
Polymerase Chain ◽  
Single Heater

A Reusable Flow-Through Polymerase Chain Reaction Instrument for the Continuous Monitoring of Infectious Biological Agents

2003 ◽  
Vol 75 (14) ◽  
pp. 3446-3450 ◽  
Author(s):  
Phillip Belgrader ◽  
Christopher J. Elkin ◽  
Steven B. Brown ◽  
Shanavaz N. Nasarabadi ◽  
Richard G. Langlois ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Continuous Monitoring ◽  
Biological Agents ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Instrument ◽  
Flow Through ◽  
Polymerase Chain

Renewable microcolumns for automated DNA purification and flow-through amplification: from sediment samples through polymerase chain reaction

2002 ◽  
Vol 469 (1) ◽  
pp. 129-140 ◽  
Author(s):  
Cynthia J. Bruckner-Lea ◽  
Toyoko Tsukuda ◽  
Brian Dockendorff ◽  
James C. Follansbee ◽  
Mark T. Kingsley ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Purification ◽  
Chain Reaction ◽  
Sediment Samples ◽  
Flow Through ◽  
Polymerase Chain

Flow-through polymerase chain reaction inside a seamless 3D helical microreactor fabricated utilizing a silicone tube and a paraffin mold

The Analyst ◽  
2015 ◽  
Vol 140 (5) ◽  
pp. 1416-1420 ◽  
Author(s):  
Wenming Wu ◽  
Kieu The Loan Trinh ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Hot Plate ◽  
Silicone Tube ◽  
Flow Through ◽  
Polymerase Chain

Seamless 3D helical silicone tube microreactors were fabricated for performing flow-through PCR employing a single hot plate and a portable micropump.

Portable plastic syringe as a self-actuated pump for long-distance uniform delivery of liquid inside a microchannel and its application for flow-through polymerase chain reaction on chip

RSC Advances ◽  
2015 ◽  
Vol 5 (16) ◽  
pp. 12071-12077 ◽  
Author(s):  
Wenming Wu ◽  
Kieu The Loan Trinh ◽  
Yu Zhang ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Flow Rate ◽  
Chain Reaction ◽  
Long Distance ◽  
Chip Flow ◽  
Hands On ◽  
Plastic Syringe ◽  
Flow Through ◽  
Polymerase Chain ◽  
On Chip

A strategy for realizing self-actuated pumping with uniform flow rate over a long distance is introduced using hands-on operation of disposable syringe, and was applied for on-chip flow-through PCR inside a serpentine PMMA microchannel.

scholarly journals One-heater flow-through polymerase chain reaction device by heat pipes cooling

2015 ◽  
Vol 9 (1) ◽  
pp. 014107 ◽  
Author(s):  
Jyh Jian Chen ◽  
Ming Huei Liao ◽  
Kun Tze Li ◽  
Chia Ming Shen
Keyword(s):  
Polymerase Chain Reaction ◽  
Heat Pipes ◽  
Chain Reaction ◽  
Flow Through ◽  
Polymerase Chain ◽  
Reaction Device
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