Real-time monitoring of skin wound healing on nano-grooves topography using electric cell-substrate impedance sensing (ECIS)

2017 ◽  
Vol 250 ◽  
pp. 461-468 ◽  
Author(s):  
Yao Cui ◽  
Yu An ◽  
Tongyu Jin ◽  
Fan Zhang ◽  
Pingang He
Keyword(s):  
Wound Healing ◽  
Real Time ◽  
Skin Wound ◽  
Real Time Monitoring ◽  
Skin Wound Healing ◽  
Impedance Sensing ◽  
Cell Substrate

Real-time Monitoring Skin Cell Alignment on Nano-grooves Using Electric Cell-substrate Impedance Sensing (ECIS)

2017 ◽  
Vol 75 (11) ◽  
pp. 1115
Author(s):  
Tongyu Jin ◽  
Yu An ◽  
Fan Zhang ◽  
Pingang He
Keyword(s):  
Real Time ◽  
Cell Alignment ◽  
Real Time Monitoring ◽  
Impedance Sensing ◽  
Skin Cell ◽  
Cell Substrate

scholarly journals Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Hyo Eun Park ◽  
Donghee Kim ◽  
Hyun Sook Koh ◽  
Sungbo Cho ◽  
Jung-Suk Sung ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Real Time ◽  
Neural Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Real Time Monitoring ◽  
Impedance Sensing ◽  
Cell Substrate ◽  
Cells Cultured

Stem cells are useful for cell replacement therapy. Stem cell differentiation must be monitored thoroughly and precisely prior to transplantation. In this study we evaluated the usefulness of electric cell-substrate impedance sensing (ECIS) forin vitroreal-time monitoring of neural differentiation of human mesenchymal stem cells (hMSCs). We cultured hMSCs in neural differentiation media (NDM) for 6 days and examined the time-course of impedance changes with an ECIS array. We also monitored the expression of markers for neural differentiation, total cell count, and cell cycle profiles. Cellular expression of neuron and oligodendrocyte markers increased. The resistance value of cells cultured in NDM was automatically measured in real-time and found to increase much more slowly over time compared to cells cultured in non-differentiation media. The relatively slow resistance changes observed in differentiating MSCs were determined to be due to their lower growth capacity achieved by induction of cell cycle arrest in G0/G1. Overall results suggest that the relatively slow change in resistance values measured by ECIS method can be used as a parameter for slowly growing neural-differentiating cells. However, to enhance the competence of ECIS forin vitroreal-time monitoring of neural differentiation of MSCs, more elaborate studies are needed.

Real-Time Monitoring In Vitro Cellular Cytotoxicity of Silica Nanotubes Using Electric Cell-Substrate Impedance Sensing (ECIS)

2013 ◽  
Vol 9 (2) ◽  
pp. 286-290 ◽  
Author(s):  
Trong Binh Tran ◽  
Phuong Diem Nguyen ◽  
Soong Ho Um ◽  
Sang Jun Son ◽  
Junhong Min
Keyword(s):  
Real Time ◽  
Cellular Cytotoxicity ◽  
Real Time Monitoring ◽  
Silica Nanotubes ◽  
Impedance Sensing ◽  
Cell Substrate

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

scholarly journals The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 457
Author(s):  
Andreu Blanquer ◽  
Jana Musilkova ◽  
Elena Filova ◽  
Johanka Taborska ◽  
Eduard Brynda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Chronic Wounds ◽  
Human Keratinocytes ◽  
Skin Wound ◽  
Platelet Lysate ◽  
Therapeutic Approaches ◽  
Skin Wound Healing ◽  
New Therapeutic Approaches ◽  
Proliferation And Differentiation

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

Flexible Bicolorimetric Polyacrylamide/Chitosan Hydrogels for Smart Real‐Time Monitoring and Promotion of Wound Healing

2021 ◽  
pp. 2102599
Author(s):  
Kaikai Zheng ◽  
Yu Tong ◽  
Shihao Zhang ◽  
Ruiying He ◽  
Lan Xiao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Real Time ◽  
Real Time Monitoring ◽  
Chitosan Hydrogels

scholarly journals Sus Scrofa immune tissues as a new source of bioactive substances for skin wound healing

2020 ◽  
Author(s):  
Alexandr Basov ◽  
Liliya Fedulova ◽  
Ekaterina Vasilevskaya ◽  
Ekaterina Trofimova ◽  
Nataliya Murashova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Sus Scrofa ◽  
Skin Wound ◽  
Bioactive Substances ◽  
Skin Wound Healing ◽  
Immune Tissues
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