A novel label-free fluorescence aptasensor for dopamine detection based on an Exonuclease III- and SYBR Green I- aided amplification strategy

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

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Exonuclease III–assisted cascade signal amplification strategy for label-free and ultrasensitive electrochemical detection of nucleic acids

Biosensors and Bioelectronics ◽

10.1016/j.bios.2016.09.036 ◽

2017 ◽

Vol 87 ◽

pp. 732-736 ◽

Cited By ~ 37

Author(s):

Erhu Xiong ◽

Xiaoxia Yan ◽

Xiaohua Zhang ◽

Yunqing Liu ◽

Jiawan Zhou ◽

...

Keyword(s):

Nucleic Acids ◽

Electrochemical Detection ◽

Signal Amplification ◽

Label Free ◽

Exonuclease Iii ◽

Amplification Strategy

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An enzyme-free and label-free assay for copper(ii) ion detection based on self-assembled DNA concatamers and Sybr Green I

The Analyst ◽

10.1039/c3an00973d ◽

2013 ◽

Vol 138 (17) ◽

pp. 4737 ◽

Cited By ~ 23

Author(s):

Chenchen Ge ◽

Junhua Chen ◽

Wei Wu ◽

Zhiyuan Fang ◽

Lingbo Chen ◽

...

Keyword(s):

Sybr Green ◽

Sybr Green I ◽

Label Free ◽

Ion Detection ◽

Self Assembled

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Improved Detection of Hg2+ in Aqueous Solution with High Sensitivity and Selectivity by Using Mercury-Specific DNA, Sybr Green I and Gold Nanoparticles

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.239-242.934 ◽

2011 ◽

Vol 239-242 ◽

pp. 934-939

Author(s):

Hui Xu ◽

Shuli Gao ◽

Jian Nong Chen ◽

Quan Wen Liu

Keyword(s):

Gold Nanoparticles ◽

High Sensitivity ◽

Sybr Green ◽

Sybr Green I ◽

Fluorescence Signal ◽

Potassium Ions ◽

Label Free ◽

Turn On ◽

Sensitivity And Selectivity ◽

Fluorescence Turn On

We report a label-free, fast, fluorescence turn on assay for Hg2+detecton by using mercury-specific DNA (MSD), Sybr Green I (SG) and gold nanoparticles (AuNPs). SG efficiently discriminates MSD and MSD/Hg2+complex. The addition of gold nanoparticle decreases the background fluorescence signal further for MSD. The fluorescence intensity of MSD/Hg2+complex keeps constant after addition of AuNPs. This property improves the signal-to-background ratio and decreases the detection limitation further. In addition, the method shows improved selectivity compared with that in the absence of AuNPs. This strategy could be applied to the detection of potassium ions and showed good generality.

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A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽

10.1039/c4an00389f ◽

2014 ◽

Vol 139 (11) ◽

pp. 2884-2889 ◽

Cited By ~ 13

Author(s):

Xingti Liu ◽

Qingwang Xue ◽

Yongshun Ding ◽

Jing Zhu ◽

Lei Wang ◽

...

Keyword(s):

Detection Method ◽

Dna Detection ◽

Rolling Circle Amplification ◽

Label Free ◽

Exonuclease Iii ◽

Rolling Circle ◽

Free Dna ◽

Exo Iii ◽

Amplification Strategy ◽

Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

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Exonuclease III-Assisted Cascade Signal Amplification Strategy for Label-Free and Ultrasensitive Chemiluminescence Detection of DNA

Analytical Chemistry ◽

10.1021/ac5024952 ◽

2014 ◽

Vol 86 (17) ◽

pp. 8881-8887 ◽

Cited By ~ 102

Author(s):

Yuan Gao ◽

Baoxin Li

Keyword(s):

Signal Amplification ◽

Label Free ◽

Chemiluminescence Detection ◽

Exonuclease Iii ◽

Amplification Strategy

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A dichromatic label-free aptasensor for sulfadimethoxine detection in fish and water based on AuNPs color and fluorescent dyeing of double-stranded DNA with SYBR Green I

Food Chemistry ◽

10.1016/j.foodchem.2019.125712 ◽

2020 ◽

Vol 309 ◽

pp. 125712 ◽

Cited By ~ 7

Author(s):

Xiang-Xiu Chen ◽

Zheng-Zhong Lin ◽

Cheng-Yi Hong ◽

Qiu-Hong Yao ◽

Zhi-Yong Huang

Keyword(s):

Sybr Green ◽

Sybr Green I ◽

Label Free ◽

Double Stranded Dna ◽

Water Based

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Exonuclease III assisted and label-free detection of mercury ion based on toehold strand displacement amplification strategy

Analytical Methods ◽

10.1039/c6ay02169g ◽

2016 ◽

Vol 8 (39) ◽

pp. 7054-7060 ◽

Cited By ~ 5

Author(s):

Lili Yu ◽

Hui Xu ◽

Hou Chen ◽

Liangjiu Bai ◽

Wenxiang Wang

Keyword(s):

High Sensitivity ◽

Mercury Ion ◽

Fluorometric Assay ◽

Label Free ◽

Strand Displacement ◽

Strand Displacement Amplification ◽

Exonuclease Iii ◽

Label Free Detection ◽

Sensitivity And Selectivity ◽

Amplification Strategy

A label-free, exonuclease III assisted Hg2+ fluorometric assay based on strand displacement amplification was developed with high sensitivity and selectivity.

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