In situ target enzyme-activated near-infrared fluorescent probe: A case study of CYP2J2 using three-fragmentary molecular assembly engineering

2021 ◽  
Vol 328 ◽  
pp. 129034 ◽  
Author(s):  
Yanpeng Dai ◽  
Tianzi Xue ◽  
Hefang Ji ◽  
Pan Zhang ◽  
Dongdong Zhang ◽  
...  
2021 ◽  
Vol 136 (2) ◽  
Author(s):  
Dario Barni ◽  
Luisa Raimondo ◽  
Anna Galli ◽  
Rossella Yivlialin ◽  
Simone Caglio ◽  
...  

AbstractAcrylic colors are mixtures of several components that can be identified as pigments, binders, and fillers, so that, when analyzed, the characteristic response of the different components may not be recognizable. This limits the accuracy of spectroscopic techniques, nonetheless particularly useful as they are noninvasive and can be applied in situ on real artworks. Here, a method is proposed to chemically separate and identify the different components of acrylic colors, in order to be able to study their spectroscopic response separately, in particular by ultraviolet–visible–near-infrared diffuse reflectance. The results clearly show that the chemical and analytical method developed here is fully reliable, with the advantage of clearly separating the response of the different components without any change of their chromatic/chemical properties. As a case study, the new method is applied here to original acrylic colors used by the Italian artist Ico Parisi, in view of building a spectra database.


2019 ◽  
Vol 91 (16) ◽  
pp. 10901-10907 ◽  
Author(s):  
Yang Tian ◽  
Yongfei Li ◽  
Wen-Li Jiang ◽  
Dong-Ye Zhou ◽  
Junjie Fei ◽  
...  

2017 ◽  
Vol 8 (4) ◽  
pp. 2795-2803 ◽  
Author(s):  
Zi-Ru Dai ◽  
Lei Feng ◽  
Qiang Jin ◽  
Hailing Cheng ◽  
Yan Li ◽  
...  

A practical strategy was proposed and successfully used to design and develop an isoform-specific probe for a given enzyme.


2020 ◽  
Vol 92 (3) ◽  
pp. 2802-2808 ◽  
Author(s):  
Songjiao Li ◽  
Dan Song ◽  
Weijing Huang ◽  
Zhen Li ◽  
Zhihong Liu

2020 ◽  
Vol 8 (9) ◽  
pp. 1914-1921 ◽  
Author(s):  
Lintao Zeng ◽  
Tianhong Chen ◽  
Bao-Quan Chen ◽  
Hou-Qun Yuan ◽  
Ruilong Sheng ◽  
...  

A mitochondria-targeting and in situ-activatable near-infrared fluorescent probe has been developed for imaging sulfur dioxide derivatives and their fluctuations in vivo.


Heritage ◽  
2019 ◽  
Vol 2 (1) ◽  
pp. 467-489
Author(s):  
Dimitris Roubis ◽  
Claudia Sciuto

The study of stone artifacts and their provenance is an important proxy for understanding the entangled relationship between humans and geological resources. In this paper, we explore the potentialities of an interdisciplinary approach combining in situ documentation of tool marks and characterization of stone types using a near infrared (NIR) portable probe. We argue that this protocol is useful for collecting screening data on objects that cannot be moved or sampled. NIR spectra describe textural and molecular features of the materials and can be used to achieve a preliminary characterization of raw materials. We present a case study from the territory of Montescaglioso (Basilicata, near Matera, Italy), where we combined the analysis of a calcarenite (limestone) quarry, in Masseria D’Alessio, which was exploited since the 6th century BC, as well as artifacts of the same chronology from surveys and excavations in the surroundings. The aim was to collect preliminary data about the distribution of the particular calcarenite extracted from the quarry and identify exploitation and trade patterns. The data were processed using multivariate statistics to highlight the relevant spectral information and perform supervised classification of spectral features. Documentation of tool marks and the process of stone working were combined with the spectral signature of the artifacts to link the stone types to the description of their extraction/carving methods.


2019 ◽  
Vol 10 (9) ◽  
pp. 2805-2810 ◽  
Author(s):  
Ping Li ◽  
Jijuan Wang ◽  
Xin Wang ◽  
Qi Ding ◽  
Xiaoyi Bai ◽  
...  

A near-infrared fluorescent probe was exploited to sensitively visualize ozone in the brains of mice with depression phenotypes in situ.


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