Label-free quantitative lymphocyte activation profiling using microfluidic impedance cytometry

2021 ◽  
pp. 129864
Author(s):  
Chayakorn Petchakup ◽  
Paul Edward Hutchinson ◽  
Hui Min Tay ◽  
Sheng Yuan Leong ◽  
King Ho Holden Li ◽  
...  
Keyword(s):  
Lymphocyte Activation ◽  
Label Free ◽  
Impedance Cytometry

scholarly journals Integrated inertial-impedance cytometry for rapid label-free leukocyte isolation and profiling of neutrophil extracellular traps (NETs)

Lab on a Chip ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 1736-1746 ◽  
Author(s):  
Chayakorn Petchakup ◽  
Hui Min Tay ◽  
King Ho Holden Li ◽  
Han Wei Hou
Keyword(s):  
Neutrophil Extracellular Traps ◽  
Neutrophil Extracellular Trap ◽  
Label Free ◽  
Extracellular Traps ◽  
Trap Formation ◽  
Extracellular Trap ◽  
Impedance Cytometry

A novel integrated inertial-impedance cytometer for rapid and label-free electrical profiling of neutrophil extracellular trap formation (NETosis).

scholarly journals Microfluidics: Direct and Label‐Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry (Small 21/2021)

Small ◽  
2021 ◽  
Vol 17 (21) ◽  
pp. 2170101
Author(s):  
Lingyan Gong ◽  
Chayakorn Petchakup ◽  
Pujiang Shi ◽  
Pei Leng Tan ◽  
Lay Poh Tan ◽  
...  
Keyword(s):  
Free Cell ◽  
Label Free ◽  
Status Monitoring ◽  
Impedance Cytometry

scholarly journals Electrophysiology-based stratification of pancreatic tumorigenicity by label-free single-cell impedance cytometry

2020 ◽  
Vol 1101 ◽  
pp. 90-98 ◽  
Author(s):  
J.S. McGrath ◽  
C. Honrado ◽  
J.H. Moore ◽  
S.J. Adair ◽  
W.B. Varhue ◽  
...  
Keyword(s):  
Single Cell ◽  
Label Free ◽  
Cell Impedance ◽  
Impedance Cytometry

scholarly journals Impedance Characterization of Particles One by One Using a Nanosensor Electronic Platform

2021 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Diana Isabel Sandoval Bojorquez ◽  
Eduardo Sergio Oliveros Mata ◽  
Julian Schütt ◽  
Michael Bachmann ◽  
Larysa Baraban
Keyword(s):  
Single Cell Analysis ◽  
Electrical Signal ◽  
Microfluidic Channels ◽  
Cell Analysis ◽  
Label Free ◽  
Dielectric Characteristics ◽  
Label Free Detection ◽  
Impedance Cytometry ◽  
Impedance Characterization

Impedance cytometry represents a technique that allows the electronic characterization of colloids and living cells in a highly miniaturized way. In contrast with impedance spectroscopy, the measurements are performed at a fixed frequency, providing real-time monitoring of the species traveling over the sensor. By measuring the electrical properties of particles in suspension, the dielectric characteristics (electric conductivity and capacitance) of both cells and particles can be readily determined. During the last years, this technique has been broadly investigated; however, it is still not trivial to differentiate particles of similar size based on their dielectric characteristics. A way to increase the discrimination abilities of this technique could be the integration of nanostructures into the impedance platforms. In this work, we present the impedance cytometry study of particles using microfluidic channels aligned over interdigitated gold nanowire structures as our impedimetric sensor. The characterization of particles of different sizes and their comparison with particles of different compositions will provide an understanding of the correlation between the electrical signal and the characteristics of each particle. This approach is an attractive element for label-free detection platforms that can be integrated into lab-on-a-chip systems and further implemented for single-cell analysis.

Direct and Label‐Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry

Small ◽  
2021 ◽  
pp. 2007500
Author(s):  
Lingyan Gong ◽  
Chayakorn Petchakup ◽  
Pujiang Shi ◽  
Pei Leng Tan ◽  
Lay Poh Tan ◽  
...  
Keyword(s):  
Free Cell ◽  
Label Free ◽  
Status Monitoring ◽  
Impedance Cytometry

scholarly journals SINGLE CELL IMPEDANCE CYTOMETRY FOR RAPID AND LABEL-FREE MONOCYTE PHENOTYPING

2017 ◽  
Author(s):  
King Ho Holden Li ◽  
Chayakorn Petchakup ◽  
Hui Min Tay ◽  
Nishanth Menon ◽  
Han Wei Hou
Keyword(s):  
Single Cell ◽  
Label Free ◽  
Cell Impedance ◽  
Impedance Cytometry

scholarly journals Integrated Field’s metal microelectrodes based microfluidic impedance cytometry for cell-in-droplet quantification

2018 ◽  
Author(s):  
Jatin Panwar ◽  
Rahul Roy
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Microfluidic Channel ◽  
High Sensitivity ◽  
Cell Detection ◽  
Label Free ◽  
Single Cell Detection ◽  
Electrode Fabrication ◽  
Impedance Cytometry

AbstractMicrofluidic impedance cytometry (MIC) provides a non-optical and label-free method for single cell detection and classification in microfluidics. However, the cleanroom intensive infrastructure required for MIC electrode fabrication limits its wide implementation in microfluidic analysis. To bypass the conventional metal (platinum) electrode fabrication protocol, we fabricated coplanar ‘in-contact’ Field’s metal (icFM) microelectrodes in multilayer elastomer devices with a single photolithography step. Our icFM microelectrodes displayed excellent and comparable performance to the platinum electrodes for detection of single erythrocytes with a lock-in amplifier based MIC setup. We further characterized it for water-in-oil droplets generated in a T-junction microfluidic channel and found high sensitivity and long-term operational stability of these electrodes. Finally, to facilitate droplet based single cell analysis, we demonstrate detection and quantification of single cells entrapped in aqueous droplets.

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

A microfluidic device with integrated impedance detection for λ-DNA

2003 ◽  
Vol 773 ◽  
Author(s):  
Myung-Il Park ◽  
Jonging Hong ◽  
Dae Sung Yoon ◽  
Chong-Ook Park ◽  
Geunbae Im
Keyword(s):  
Microfluidic Device ◽  
Electrochemical Impedance ◽  
Direct Detection ◽  
Full Potential ◽  
Label Free ◽  
Buffer Solution ◽  
Detection Systems ◽  
Significant Difference ◽  
Detection Of Biomolecules ◽  
Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

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