Multiplex assays of bladder cancer protein markers with magnetic structural color hydrogel microcarriers based on microfluidics

Abstract Artificial micromotors have a demonstrated value in the biomedical area. Attempts to develop this technology tend to impart micromotors with novel functions to improve the values. Herein, we present novel structural color-barcode micromotors for the multiplex assays. We found that, by rapidly extracting solvent and assembling monodispersed nanoparticles in droplets, it could form stomatocyte colloidal crystal clusters, which not only showed striking structural colors and characteristic reflection peaks due to their ordered nanoparticles arrangement, but also provided effective cavities for the integration of functional elements. Thus, the micromotors with catalysts or magnetic elements in their cavities, as well as with the corresponding structural color coding, could be achieved by using the platinum and ferric oxide dispersed pre-gel to fill and duplicate the stomatocyte colloidal crystal clusters. We have demonstrated that the self-movement of these structural color-barcode micromotors could efficiently accelerate the mixing speed of the detection sample and greatly increase the probe–target interactions towards faster and more sensitive single or multiplex detection, and the magnetism of these barcode micromotors enables the flexible collection of the micromotors, which could facilitate the detection processes. These features make the stomatocyte structural color-barcode micromotors ideal for biomedical applications.

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Abstract 300: Identification of a panel of protein markers associated with disease progression in bladder cancer

10.1158/1538-7445.am2011-300 ◽

2011 ◽

Author(s):

Karin Birkenkamp-Demtroder ◽

Lars Dyrskjøt ◽

Francisco Mansilla ◽

Torben F. Ørntoft

Keyword(s):

Bladder Cancer ◽

Disease Progression ◽

Protein Markers

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Comparison of Commercial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based Immunoassay for Detecting a Urine-Based Bladder-Cancer-Associated Diagnostic Signature

Diagnostics ◽

10.3390/diagnostics9040166 ◽

2019 ◽

Vol 9 (4) ◽

pp. 166

Author(s):

Hideki Furuya ◽

Ian Pagano ◽

Keanu Chee ◽

Takashi Kobayashi ◽

Regan S. Wong ◽

...

Keyword(s):

Bladder Cancer ◽

Matrix Metalloproteinase ◽

Protein Array ◽

Enzyme Linked Immunosorbent Assay ◽

Operating Characteristics ◽

Predictive Values ◽

Multiplex Assays ◽

Diagnostic Signature ◽

Technical Specifications ◽

Multiplex Bead

The ability to accurately measure multiple proteins simultaneously in a single assay has the potential to markedly improve the efficiency of clinical tests composed of multiple biomarkers. We investigated the diagnostic accuracy of the two multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 subjects (40 with bladder cancer). Banked urine samples collected from Kyoto and Nara Universities were compared to histologically determined bladder cancer. The concentrations of the 10 proteins (A1AT; apolipoprotein E—APOE; angiogenin—ANG; carbonic anhydrase 9—CA9; interleukin 8—IL-8; matrix metalloproteinase 9—MMP-9; matrix metalloproteinase 10—MMP10; plasminogen activator inhibitor 1—PAI-1; syndecan—SDC1; and vascular endothelial growth factor—VEGF) were monitored using two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s technical specifications. The range for detecting each biomarker was improved in the multiplex assays, even though the lower limit of quantification (LLOQ) was typically lower in the commercial ELISA kits. The area under the receiver operating characteristics (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA). The sensitivities and specificities for MBA were 0.93 and 0.95, respectively, and for MEA were 0.85 and 0.80, respectively. Accuracy, positive predictive values (PPV), and negative predictive values (NPV) for MBA were 0.94, 0.95, and 0.93, respectively, and for MEA were 0.83, 0.81, and 0.84, respectively. Based on these encouraging preliminary data, we believe that a multiplex protein array is a viable platform that can be utilized as an efficient and highly accurate tool to quantitate multiple proteins within biologic specimens.

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Erratum to “Mass spectrometric detection combined with bioinformatic analysis identified possible protein markers and key pathways associated with bladder cancer” [Gene 626C (2017) 407–413]

Gene ◽

10.1016/j.gene.2017.08.007 ◽

2017 ◽

Vol 631 ◽

pp. 68-69

Author(s):

Ziyu Wu ◽

Sugui Wang ◽

Fujin Jiang ◽

Qiang Li ◽

Chao Wang ◽

...

Keyword(s):

Bladder Cancer ◽

Bioinformatic Analysis ◽

Mass Spectrometric ◽

Mass Spectrometric Detection ◽

Cancer Gene ◽

Protein Markers ◽

Key Pathways

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Abstract 3656: Cathepsin E, Maspin, PLK1, and Survivin are promising prognostic protein markers for progression in non-muscle invasive bladder cancer

10.1158/1538-7445.am2012-3656 ◽

2012 ◽

Author(s):

Niels Fristrup ◽

Benedicte Parm Ulhøi ◽

Karin Birkenkamp-Demtröder ◽

Francisco Mansilla ◽

Marta Sanchez-Carbayo ◽

...

Keyword(s):

Bladder Cancer ◽

Invasive Bladder Cancer ◽

Protein Markers ◽

Muscle Invasive Bladder Cancer ◽

Cathepsin E ◽

Muscle Invasive

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Panel of Urinary Diagnostic Markers for Non-Invasive Detection of Primary and Recurrent Urothelial Urinary Bladder Carcinoma

Urologia Internationalis ◽

10.1159/000368166 ◽

2015 ◽

Vol 95 (1) ◽

pp. 56-64 ◽

Cited By ~ 18

Author(s):

Viktor Soukup ◽

Marta Kalousová ◽

Otakar Capoun ◽

Roman Sobotka ◽

Zuzana Breyl ◽

...

Keyword(s):

Bladder Cancer ◽

Cancer Recurrence ◽

Annexin V ◽

Clinical Situation ◽

Enzyme Linked Immunosorbent Assay ◽

Urinary Bladder Carcinoma ◽

Protein Markers ◽

Urine Sediment ◽

Primary Diagnostics ◽

Lower Accuracy

Objectives: To determine the combination of urinary protein markers for noninvasive detection of primary and recurrent urothelial bladder carcinomas. Methods: Urinary concentrations of 27 biomarkers (NSE, ATT, AFABP, Resistin, Midkine, Clusterin, Uromodulin, ZAG2, HSP27, HSP 60, NCAM1/CD56, Angiogenin, Calreticulin, Chromogranin A, CEACAM1, CXCL1, IL13Ra2, Progranulin, VEGFA, CarbAnhydIX, Annexin-V, TIM4, Galectin1, Cystatin B, Synuclein G, ApoA1 and ApoA2) were assessed by enzyme-linked immunosorbent assay or by electrochemiluminiscence immunoassay. Results: During the primary diagnostics, a group of 70 patients with primary occurrence of bladder cancer and 49 healthy control subjects were compared. For this clinical situation, the most accurate combination proved to be the combination of cytology with markers Midkine and Synuclein G (sensitivity 91.8%, specificity 97.5%). During the monitoring of patients with non-muscle invasive bladder cancer (NMIBC), a group of 44 patients with cancer recurrence was compared with the group of 61 patients with a history of NMIBC without current disease. For this clinical situation, the most accurate combination proved to be the combination of cytology and erythrocytes count in urine sediment with markers Midkine, ZAG2, CEACAM1, and Synuclein G (sensitivity 92.68%, specificity 90.16%). A lower accuracy of the diagnostic panel and the necessity to use more markers in the case of recurrence was connected with a different structure of patients. Conclusions: Multi-marker test can significantly improve the bladder cancer detection both during the primary diagnostics and monitoring of patients with NMIBC. This outcome should result in other, larger studies.

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Responsive Janus Structural Color Hydrogel Micromotors for Label-Free Multiplex Assays

Research ◽

10.34133/2021/9829068 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Huan Wang ◽

Lijun Cai ◽

Dagan Zhang ◽

Luoran Shang ◽

Yuanjin Zhao

Keyword(s):

Detection Efficiency ◽

Multiplex Assay ◽

Detection Sensitivity ◽

Structural Color ◽

Label Free ◽

Structural Colors ◽

Multiplex Assays ◽

Highly Sensitive ◽

Label Free Detection ◽

Coding Capacity

Micromotors with self-propelling ability demonstrate great values in highly sensitive analysis. Developing novel micromotors to achieve label-free multiplex assay is particularly intriguing in terms of detection efficiency. Herein, structural color micromotors (SCMs) were developed and employed for this purpose. The SCMs were derived from phase separation of droplet templates and exhibited a Janus structure with two distinct sections, including one with structural colors and the other providing catalytic self-propelling functions. Besides, the SCMs were functionalized with ion-responsive aptamers, through which the interaction between the ions and aptamers resulted in the shift of the intrinsic color of the SCMs. It was demonstrated that the SCMs could realize multiplex label-free detection of ions based on their optical coding capacity and responsive behaviors. Moreover, the detection sensitivity was greatly improved benefiting from the autonomous motion of the SCMs which enhanced the ion-aptamer interactions. We anticipate that the SCMs can significantly promote the development of multiplex assay and biomedical fields.

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