scholarly journals Butyribacter intestini gen. nov., sp. nov., a butyric acid-producing bacterium of the family Lachnospiraceae isolated from human faeces, and reclassification of Acetivibrio ethanolgignens as Acetanaerobacter ethanolgignens gen. nov., comb. nov

2021 ◽  
Vol 44 (3) ◽  
pp. 126201
Author(s):  
Yuanqiang Zou ◽  
Wenbin Xue ◽  
Xiaoqian Lin ◽  
Mei Lv ◽  
Guangwen Luo ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Human Faeces ◽  
The Family

Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces.

2020 ◽  
Author(s):  
CC Kim ◽  
WJ Kelly ◽  
ML Patchett ◽  
GW Tannock ◽  
Z Jordens ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Middle Lamella ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Citrus Peel ◽  
Human Faeces ◽  
The Family

© 2017 IUMS. A novel anaerobic pectinolytic bacterium (strain 14T) was isolated from human faeces. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 14T belonged to the family Ruminococcaceae, but was located separately from known clostridial clusters within the taxon. The closest cultured relative of strain 14T was Acetivibrio cellulolyticus (89.7% sequence similarity). Strain 14T shared ~99% sequence similarity with cloned 16S rRNA gene sequences from uncultured bacteria derived from the human gut. Cells were Gram-stain-positive, non-motile cocci approximately 0.6μm in diameter. Strain 14T fermented pectins from citrus peel, apple, and kiwifruit as well as carbohydrates that are constituents of pectins and hemicellulose, such as galacturonic acid, xylose, and arabinose. TEM images of strain 14T, cultured in association with plant tissues, suggested extracellular fibrolytic activity associated with the bacterial cells, forming zones of degradation in the pectin-rich regions of middle lamella. Phylogenetic and phenotypic analysis supported the differentiation of strain 14T as a novel genus in the family Ruminococcaceae. The name Monoglobus pectinilyticus gen. nov., sp. nov. is proposed; the type strain is 14T (JCM 31914T=DSM 104782T).

scholarly journals Micropropagation and conservation of Rheum webbianum collected from Zanaskar valley via tissue culture

2018 ◽  
Vol 7 (4) ◽  
pp. 2187
Author(s):  
Shagoon Tabin Khan ◽  
Azra N. Kamili ◽  
Gupta R.C.
Keyword(s):  
Tissue Culture ◽  
Medicinal Plant ◽  
Butyric Acid ◽  
Uttar Pradesh ◽  
Himachal Pradesh ◽  
Dichlorophenoxyacetic Acid ◽  
Jammu And Kashmir ◽  
The Family ◽  
Important Medicinal Plant ◽  
Benzyl Amino Purine

Rheum webbianum is one of the famous and popular medicinal plant found in Ladakh region. Rheum webbianum Royle is an important medicinal plant belonging to the family Polygonaceae. It is commonly known as ‘Himalayan Rhubarb’ in English, ‘Revandchini’ in Hindi, ‘xu mi da huang’ in Chinese, and ‘Lachhu’ or ‘Chu-rtsa’ in Ladakh. It is native to Asia-Temperate to Asia-Tropical, from China to India, Nepal and Pakistan. In India, it is found in Himachal Pradesh, Jammu and Kashmir, Uttar Pradesh. In Jammu and Kashmir, it is grown on open slopes and shrubberies. Due to overexploitation the Rheum webbianum has become endangered. so, it was conserved by the tissue culture and showed best results on different phytohormones. The plant formation was observed on combination of 6-Benzyl Amino Purine, Thidiazuron, 2, 4-Dichlorophenoxyacetic acid and Indole 3-butyric acid.

Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces.

2020 ◽  
Author(s):  
CC Kim ◽  
WJ Kelly ◽  
ML Patchett ◽  
GW Tannock ◽  
Z Jordens ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Middle Lamella ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Citrus Peel ◽  
Human Faeces ◽  
The Family

© 2017 IUMS. A novel anaerobic pectinolytic bacterium (strain 14T) was isolated from human faeces. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 14T belonged to the family Ruminococcaceae, but was located separately from known clostridial clusters within the taxon. The closest cultured relative of strain 14T was Acetivibrio cellulolyticus (89.7% sequence similarity). Strain 14T shared ~99% sequence similarity with cloned 16S rRNA gene sequences from uncultured bacteria derived from the human gut. Cells were Gram-stain-positive, non-motile cocci approximately 0.6μm in diameter. Strain 14T fermented pectins from citrus peel, apple, and kiwifruit as well as carbohydrates that are constituents of pectins and hemicellulose, such as galacturonic acid, xylose, and arabinose. TEM images of strain 14T, cultured in association with plant tissues, suggested extracellular fibrolytic activity associated with the bacterial cells, forming zones of degradation in the pectin-rich regions of middle lamella. Phylogenetic and phenotypic analysis supported the differentiation of strain 14T as a novel genus in the family Ruminococcaceae. The name Monoglobus pectinilyticus gen. nov., sp. nov. is proposed; the type strain is 14T (JCM 31914T=DSM 104782T).

scholarly journals Butyribacter intestini gen. nov., sp. nov., a butyric acid-producing bacterium of the family Lachnospiraceae isolated from the human faeces, and reclassification of Acetivibrio ethanolgignens as Acetanaerobacter ethanolgignens gen. nov., comb. nov

2020 ◽  
Author(s):  
Wenbin Xue ◽  
Xiaoqian Lin ◽  
Mei Lv ◽  
Guangwen Luo ◽  
Ying Dai ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Whole Genome Sequence ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Human Faeces ◽  
Obligate Anaerobic

AbstractA novel, non-motile, Gram-stain-positive, non-spore-forming, obligate anaerobic bacterium, designated strain TF01-11T, was isolated from human faeces. The isolate was characterized by phylogenetic and phenotypic properties, as well as by determination of its whole genome sequence. The growth temperature and pH ranges were 30–42 °C and 6.0–8.5, respectively. The end products of glucose fermentation were butyric acid and a small amount of acetic acid. The genome was estimated to be 3.61Mbp with G+C content of 36.79 mol%. Genes related to biosynthesis of diaminopimelic acid, polar lipids, polyamines, teichoic and lipoteichoic acids were present. The predominant fatty acids were C16:0 (37.9 %), C14:0 (16.4 %), C13:0 OH/iso-C15:1 H (11.1 %) and C18:1ω9c (10.6 %). Phylogenetic analyses based on 16S rRNA gene sequences, the isolate was a member of family Lachnospiraceae, with the highest sequence similarity to the type strain of Roseburia intestinalis DSM 14610T at 92.18 % followed by Acetivibrio ethanolgignens ATCC 33324T at 91.99 %. The average nucleotide identity (ANI) calculated for the genomes between strain TF01-11T and these closest relatives were 70.5 % and 68.1 %. Based on results of phenotypic characteristics and genotypic properties presented in this study, strain TF01-11T represent a novel species in a new genus, for which the name Butyribacter intestini gen. nov., sp. nov. is proposed. The type strain of the type species is TF01-11T (CGMCC 1.5203T = CGMCC 10984T = DSM 105140T). In addition, Acetivibrio ethanolgignens is proposed to be reclassified as Acetanaerobacter ethanolgignens gen. nov., comb. nov.

scholarly journals Urban blow flies (Diptera: Calliphoridae) in four cities of the Colombian Caribbean coast

Intropica ◽  
2014 ◽  
Vol 9 ◽  
pp. 84 ◽  
Author(s):  
Melissa Santodomingo-M ◽  
Adriana Santodomingo-Santodomingo ◽  
César Valverde-C
Keyword(s):  
Species Composition ◽  
Lucilia Sericata ◽  
Caribbean Coast ◽  
Blow Flies ◽  
Human Faeces ◽  
Chrysomya Megacephala ◽  
Chrysomya Albiceps ◽  
Cochliomyia Macellaria ◽  
The Family

The purpose of this study was to evaluate the species composition of the family Calliphoridae from samples collected in four different cities in the Colombian Caribbean coast. Van Someren-Rydon traps were used baited with human faeces, rotten fish and fermented fruit were used. Six traps were placed in each city (two traps per type of bait), for a total of 24 traps. They were left for 72 hours in each site and samples were collected every 12 hours (day and night). 5654 individuals were identified, belonging to the subfamilies Chrysomyinae and Luciliinae. The identified species were Cochliomyia macellaria, Chrysomya albiceps, Chrysomya megacephala, Lucilia eximia, Lucilia sericata and Chloroprocta idioidea, expanding the range of distribution for the last two species. The best bait was the rotten fish and the best time to collect these species was during daylight.

scholarly journals Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

2016 ◽  
Vol 66 (8) ◽  
pp. 3041-3049 ◽  
Author(s):  
Andrei N. Shkoporov ◽  
Andrei V. Chaplin ◽  
Victoria A. Shcherbakova ◽  
Natalia E. Suzina ◽  
Lyudmila I. Kafarskaia ◽  
...  
Keyword(s):  
Human Faeces ◽  
The Family

scholarly journals A third gyrovirus species in human faeces

2012 ◽  
Vol 93 (6) ◽  
pp. 1356-1361 ◽  
Author(s):  
Tung G. Phan ◽  
Linlin Li ◽  
Miguel G. O’Ryan ◽  
Hector Cortes ◽  
Nora Mamani ◽  
...  
Keyword(s):  
Metagenomic Analysis ◽  
Chicken Anemia Virus ◽  
Human Pathogens ◽  
Human Faeces ◽  
Healthy Humans ◽  
The Family ◽  
High Prevalence ◽  
Anemia Virus

Until 2011 the genus Gyrovirus in the family Circoviridae consisted of a single virus (Chicken anemia virus or CAV) causing a common immunosuppressive disease in chickens when a second gyrovirus (HGyV) was reported on the skin of 4 % of healthy humans. HGyV is very closely related to a recently described chicken gyrovirus, AGV2, suggesting that they belong to the same viral species. During a viral metagenomic analysis of 100 human faeces from children with diarrhoea in Chile we identified multiple known human pathogens (adenoviruses, enteroviruses, astroviruses, sapoviruses, noroviruses, parechoviruses and rotaviruses) and a novel gyrovirus species we named GyV3 sharing <63 % similarity with other gyrovirus proteins with evidence of recombination with CAV in its UTR. Gyroviridae consensus PCR revealed a high prevalence of CAV DNA in diarrhoea and normal faeces from Chilean children and faeces of USA cats and dogs, which may reflect consumption of CAV-infected/vaccinated chickens. Whether GyV3 can infect humans and/or chickens requires further studies.

scholarly journals Mesosutterella multiformis gen. nov., sp. nov., a member of the family Sutterellaceae and Sutterella megalosphaeroides sp. nov., isolated from human faeces

2018 ◽  
Vol 68 (12) ◽  
pp. 3942-3950 ◽  
Author(s):  
Mitsuo Sakamoto ◽  
Nao Ikeyama ◽  
Tadao Kunihiro ◽  
Takao Iino ◽  
Masahiro Yuki ◽  
...  
Keyword(s):  
Human Faeces ◽  
The Family
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