Integrative analysis of genes and miRNA alterations in human embryonic stem cells-derived neural cells after exposure to silver nanoparticles

2016 ◽  
Vol 299 ◽  
pp. 8-23 ◽  
Author(s):  
Jung-Hwa Oh ◽  
Mi-Young Son ◽  
Mi-Sun Choi ◽  
Soojin Kim ◽  
A-young Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Silver Nanoparticles ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Integrative Analysis ◽  
Neural Cells

scholarly journals Comprehensive Gene Expression Analysis of Human Embryonic Stem Cells during Differentiation into Neural Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e22856 ◽  
Author(s):  
Ali Fathi ◽  
Maryam Hatami ◽  
Vahid Hajihosseini ◽  
Faranak Fattahi ◽  
Sahar Kiani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Expression Analysis ◽  
Human Embryonic Stem Cells ◽  
Gene Expression Analysis ◽  
Embryonic Stem ◽  
Neural Cells

Neural Differentiation of Human Embryonic Stem Cells at the Ultrastructural Level

2010 ◽  
Vol 16 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Jennifer L. Mumaw ◽  
Dave Machacek ◽  
John P. Shields ◽  
Mahesh C. Dodla ◽  
Sujoy K. Dhara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Ultrastructural Level ◽  
Culture Conditions ◽  
Ultrastructural Changes ◽  
Neural Cells ◽  
Cell Therapies

AbstractNeurodegerative disorders affect millions of people worldwide. Neural cells derived from human embryonic stem cells (hESC) have the potential for cell therapies and/or compound screening for treating affected individuals. While both protein and gene expression indicative of a neural phenotype has been exhibited in these differentiated cells, ultrastuctural studies thus far have been lacking. The objective of this study was to correlate hESC to neural differentiation culture conditions with ultrastructural changes observed in the treated cells. We demonstrate here that in basic culture conditions without growth factors or serum we obtain neural morphology. The addition of brain-derived neurotrophic factor (BDNF) and serum to cultures resulted in more robust neural differentiation. In addition to providing cues such as cell survival or lineage specification, additional factors also altered the intracellular structures and cell morphologies. Even though the addition of BDNF and serum did not increase synaptic formation, altered cellular structures such as abundant polyribosomes and more developed endoplasmic reticulum indicate a potential increase in protein production.

scholarly journals Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells

Stem Cells ◽  
2012 ◽  
Vol 30 (11) ◽  
pp. 2387-2399 ◽  
Author(s):  
Anirban Majumder ◽  
Saswati Banerjee ◽  
Joshua A. Harrill ◽  
David W. Machacek ◽  
Osama Mohamad ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Leukemia Inhibitory Factor ◽  
Embryonic Stem ◽  
Inhibitory Factor ◽  
Neural Cells

scholarly journals Dominant-Negative Effects of Adult-Onset Huntingtin Mutations Alter the Division of Human Embryonic Stem Cells-Derived Neural Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0148680 ◽  
Author(s):  
Carla Lopes ◽  
Sophie Aubert ◽  
Fany Bourgois-Rocha ◽  
Monia Barnat ◽  
Ana Cristina Rego ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Dominant Negative ◽  
Neural Cells ◽  
Adult Onset ◽  
Negative Effects
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