Biosorption: A new rise for elemental solid phase extraction methods

NanoMIPs that are prepared by solid phase synthesis have proven to be very versatile, but to date only limited attention has been paid to their use in solid phase extraction. Thus, since nanoMIPs show close similarities, in terms of binding behavior, to antibodies, it seems relevant to verify if it is possible to use them as mimics of the natural antibodies that are used in immunoextraction methods. As a proof-of-concept, we considered prepared nanoMIPs against fluoroquinolone ciprofloxacin. Several nanoMIPs were prepared in water with polymerization mixtures of different compositions. The polymer with the highest affinity towards ciprofloxacin was then grafted onto a solid support and used to set up a solid phase extraction–HPLC method with fluorescence detection, for the determination of fluoroquinolones in human urine. The method resulted in successful selection for the fluoroquinolone antibiotics, such that the nanoMIPs were suitable for direct extraction of the antibiotics from the urine samples at the µg mL−1 level. They required no preliminary treatment, except for a 1 + 9 (v/v) dilution with a buffer of pH 4.5 and they had good analyte recovery rates; up to 85% with precision in the range of 3 to 4.5%, without interference from the matrix. These experimental results demonstrate, for the first time, the feasibility of the use of nanoMIPs to develop solid phase extraction methods.

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Modern Approaches to Preparation of Body Fluids for Determination of Bioactive Compounds

Separations ◽

10.3390/separations6040053 ◽

2019 ◽

Vol 6 (4) ◽

pp. 53 ◽

Cited By ~ 3

Author(s):

Katarzyna Madej ◽

Wojciech Piekoszewski

Keyword(s):

Liquid Phase ◽

Solid Phase Extraction ◽

Bioactive Compounds ◽

Solid Phase ◽

Magnetic Solid Phase Extraction ◽

Extraction Methods ◽

Body Fluids ◽

Phase Extraction ◽

Modern Approach ◽

Dispersive Liquid Liquid Microextraction

The current clinical and forensic toxicological analysis of body fluids requires a modern approach to sample preparation characterized by high selectivity and enrichment capability, suitability for micro-samples, simplicity and speed, and the possibility of automation and miniaturization, as well as the use of small amounts of reagents, especially toxic solvents. Most of the abovementioned features may be realized using so-called microextraction techniques which cover liquid-phase techniques (e.g., single-drop microextraction, SDME; dispersive liquid–liquid microextraction, DLLME; hollow-fiber liquid-phase microextraction, HF-LPME) and solid-phase extraction techniques (solid-phase microextraction, SPME; microextraction in packed syringes, MEPS; disposable pipette tip extraction, DPX; stir bar sorption extraction, SBSE). Some other extraction methodologies like dispersive solid-phase extraction (d-SPE) or magnetic solid-phase extraction (MSPE) can also be easily miniaturized. This review briefly describes and characterizes the abovementioned extraction methods, and then presents their current applications to the preparation of body fluids analyzed for bioactive compounds in combination with appropriate analytical methods, mainly chromatographic and related techniques. The perspectives of the analytical area we are interested in are also indicated.

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Comparison of solid phase extraction methods for the measurement of humic-like substances (HULIS) in atmospheric particles

Atmospheric Environment ◽

10.1016/j.atmosenv.2020.117370 ◽

2020 ◽

Vol 225 ◽

pp. 117370

Author(s):

Chunlin Zou ◽

Meiju Li ◽

Tao Cao ◽

Mengbo Zhu ◽

Xingjun Fan ◽

...

Keyword(s):

Solid Phase Extraction ◽

Solid Phase ◽

Extraction Methods ◽

Atmospheric Particles ◽

Phase Extraction

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Systematic Assessment of Seven Solvent and Solid-Phase Extraction Methods for Metabolomics Analysis of Human Plasma by LC-MS

Scientific Reports ◽

10.1038/srep38885 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 35

Author(s):

Dmitri G. Sitnikov ◽

Cian S. Monnin ◽

Dajana Vuckovic

Keyword(s):

Solid Phase Extraction ◽

Human Plasma ◽

Solid Phase ◽

Extraction Methods ◽

Phase Extraction ◽

Systematic Assessment ◽

Metabolomics Analysis

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Comparison of Solid-Phase Extraction and Micro-Solid-Phase Extraction for Liquid Chromatography/Mass Spectrometry Analysis of Pesticides in Water Samples

Journal of AOAC International ◽

10.1093/jaoac/86.5.941 ◽

2003 ◽

Vol 86 (5) ◽

pp. 941-946 ◽

Cited By ~ 2

Author(s):

Giorgio Famiglini ◽

Helga Trufelli ◽

Elisabetta Pierini ◽

Elisa De Simoni ◽

Filippo Mangani ◽

...

Keyword(s):

Liquid Chromatography ◽

Solid Phase Extraction ◽

Ion Trap ◽

Solid Phase ◽

Signal To Noise Ratio ◽

Extraction Methods ◽

Graphitized Carbon Black ◽

Mass Spectrometry Analysis ◽

Phase Extraction ◽

Spectrometry Analysis

Abstract Our recent on-line solid-phase extraction (SPE) device for micro-liquid chromatography, known as micro-solid-phase extraction (μSPE), was compared with traditional SPE for the analysis, from aqueous samples, of 4 pesticides belonging to different classes. Two different kinds of adsorbents, C18 and graphitized carbon black, were tested. A 2-stage ion trap mass spectrometer, equipped with homemade microflow electrospray ion (ESI) source, was used. Detection limits with a signal-to-noise ratio of 3:1 for both extraction methods were in the range of 0.1 μg/L for all compounds. However, better recoveries were obtained when μSPE traps were used.

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