Development and validation of the first high performance-lateral flow immunoassay (HP-LFIA) for the rapid screening of domoic acid from shellfish extracts

Talanta ◽  
2013 ◽  
Vol 116 ◽  
pp. 663-669 ◽  
Author(s):  
Waqass Jawaid ◽  
Julie Meneely ◽  
Katrina Campbell ◽  
Mark Hooper ◽  
Karrie Melville ◽  
...  
Keyword(s):  
High Performance ◽  
Domoic Acid ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay ◽  
Development And Validation

Development and Validation of a Lateral Flow Immunoassay for the Rapid Screening of Okadaic Acid and All Dinophysis Toxins from Shellfish Extracts

2015 ◽  
Vol 63 (38) ◽  
pp. 8574-8583 ◽  
Author(s):  
Waqass Jawaid ◽  
Julie P. Meneely ◽  
Katrina Campbell ◽  
Karrie Melville ◽  
Stephen J. Holmes ◽  
...  
Keyword(s):  
Okadaic Acid ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay ◽  
Development And Validation

scholarly journals Development and validation of a lateral flow immunoassay for rapid detection of VanA-producing enterococci

2020 ◽  
Vol 76 (1) ◽  
pp. 146-151 ◽  
Author(s):  
Saoussen Oueslati ◽  
Hervé Volland ◽  
Vincent Cattoir ◽  
Sandrine Bernabeu ◽  
Delphine Girlich ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Culture Medium ◽  
Limit Of Detection ◽  
Culture Media ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Clinical Microbiology Laboratory ◽  
Mueller Hinton ◽  
Reliable Detection ◽  
Development And Validation

Abstract Background VRE are nosocomial pathogens with an increasing incidence in recent decades. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. Objectives To evaluate a lateral flow immunoassay (LFIA) (called NG-Test VanA) for the rapid and reliable detection of VanA-producing VRE (VanA-VRE) from colonies and broth. Methods NG-Test VanA was validated on 135 well-characterized enterococcal isolates grown on Mueller–Hinton (MH) agar (including 40 VanA-VRE). Different agar plates and culture broths widely used in routine laboratories for culture of enterococci were tested. Results All 40 VanA-VRE clinical isolates were correctly detected in less than 15 min irrespective of the species expressing the VanA ligase and the medium used for bacterial growth. No cross-reaction was observed with any other clinically relevant ligases (VanB, C1, C2, D, E, G, L, M and N). Overall, the sensitivity and specificity of the assay were 100% for VanA-VRE grown on MH agar plates. NG-Test VanA accurately detects VanA-VRE irrespective of the culture medium (agar and broth). Band intensity was increased when using bacteria grown on vancomycin-containing culture media or on MH close to the vancomycin disc as a consequence of VanA induction. The limit of detection of the assay was 6.3 × 106 cfu per test with bacteria grown on MH plates and 4.9 × 105 cfu per test with bacteria grown on ChromID® VRE plates. Conclusions NG-Test VanA is efficient, rapid and easy to implement in the routine workflow of a clinical microbiology laboratory for the confirmation of VanA-VRE.

scholarly journals Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection

Biosensors ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 13
Author(s):  
Tao Peng ◽  
Xueshima Jiao ◽  
Zhanwei Liang ◽  
Hongwei Zhao ◽  
Yang Zhao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Nucleocapsid Protein ◽  
High Efficiency ◽  
Protein Detection ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Copper Deposition ◽  
Detection Sensitivity ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 μg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.

scholarly journals A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2109
Author(s):  
Zifei Wang ◽  
Pengjie Luo ◽  
Baodong Zheng
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Animal Feed ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Distillers Grains ◽  
Detection Methods ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Fluorescent Microsphere

Aflatoxin B1 (AFB1) is a toxic compound naturally produced by the genera Aspergillus. Distillers’ grains can be used as animal feed since they have high content of crude protein and other nutrients. However, they are easily contaminated by mycotoxins, and currently there are no rapid detection methods for AFB1 in distillers’ grains. In this study, a lateral flow immunoassay (LFIA) based on red fluorescent microsphere (FM), is developed for quantitative detection of AFB1 in distillers’ grains. The whole test can be completed within 15 min, with the cut-off value being 25.0 μg/kg, and the quantitative limit of detection (qLOD) being 3.4 μg/kg. This method represents satisfactory recoveries of 95.2–113.0%, and the coefficients of variation (CVs) are less than 7.0%. Furthermore, this technique is successfully used to analyze AFB1 in real samples, and the results indicates good consistency with that of high-performance liquid chromatography (HPLC). The correlation coefficient is found to be greater than 0.99. The proposed test strip facilitates on-site, cost-effective, and sensitive monitoring of AFB1 in distillers’ grains.

Detection of triclabendazole and three metabolites in bovine muscle samples with a gold nanoparticle-based lateral flow immunoassay

2019 ◽  
Vol 11 (42) ◽  
pp. 5478-5486 ◽  
Author(s):  
Zhongxing Wang ◽  
Xiaoling Wu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
Chuanlai Xu
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay ◽  
Bovine Muscle ◽  
Highly Sensitive

A highly sensitive mAb against TCD was produced by using a novel hapten, and basing on this mAb, a lateral flow immunoassay was developed for the rapid screening of TCD and its metabolites in foodstuff.

Development and validation of a novel lateral flow immunoassay device for detection of aflatoxins in soy-based foods

2017 ◽  
Vol 9 (18) ◽  
pp. 2715-2722 ◽  
Author(s):  
Vanessa O. Santos ◽  
Patrícia B. Pelegrini ◽  
Fernanda Mulinari ◽  
Ariane F. Lacerda ◽  
Rodrigo S. Moura ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Fungal Metabolites ◽  
Natural Toxins ◽  
Development And Validation

Aflatoxins (AFs) are natural toxins produced as secondary fungal metabolites.

scholarly journals Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation

Toxins ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 56 ◽  
Author(s):  
Du Wang ◽  
Jianguo Zhu ◽  
Zhaowei Zhang ◽  
Qi Zhang ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Single Step ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Tandem Mass ◽  
Chemical Contaminants ◽  
Chromatography Tandem Mass Spectrometry

Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B1 (AFB1), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 µg/kg in maize and 0.18, 0.57, and 1.47 µg/kg in peanut with an analytical range of 0.48–20, 1.56–200, and 3.63–300 µg/kg for AFB1, ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24–110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety.

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