Relationships between oxygen consumption rate, viability, and subsequent development of in vivo–derived porcine embryos

2015 ◽  
Vol 83 (1) ◽  
pp. 14-20 ◽  
Author(s):  
N. Sakagami ◽  
K. Nishida ◽  
K. Akiyama ◽  
H. Abe ◽  
H. Hoshi ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Subsequent Development

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

Nitric oxide modulates oxygen consumption by arteriolar walls in rat skeletal muscle

2005 ◽  
Vol 289 (6) ◽  
pp. H2673-H2679 ◽  
Author(s):  
Masahiro Shibata ◽  
Shigeru Ichioka ◽  
Akira Kamiya
Keyword(s):  
Nitric Oxide ◽  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Phosphorescence Quenching ◽  
Arterial Blood ◽  
No Release ◽  
Vessel Walls

To study the role of nitric oxide (NO) in regulating oxygen consumption by vessel walls, the oxygen consumption rate of arteriolar walls in rat cremaster muscle was measured in vivo during flow-induced vasodilation and after inhibiting NO synthesis. The oxygen consumption rate of arteriolar walls was calculated based on the intra- and perivascular Po2 values measured by phosphorescence quenching laser microscopy. The perivascular Po2 value of the arterioles during vasodilation was significantly higher than under control conditions, although the intravascular Po2 values under both conditions were approximately the same. Inhibition of NO synthesis, on the other hand, caused a significant increase in arterial blood pressure and a significant decrease in arteriolar diameter. Inhibition of NO synthesis also caused a significant decrease in both the intra- and perivascular Po2 values of the arterioles. Inhibition of NO synthesis increased the oxygen consumption rate of the vessel walls by 42%, whereas enhancement of flow-induced NO release decreased it by 34%. These results suggest that NO plays an important role not only as a regulator of peripheral vascular tone but also as a modulator of tissue oxygenation by reducing oxygen consumption by vessel walls. In addition, enhancement of NO release during exercise may facilitate efficient oxygen supply to the surrounding high metabolic tissue.

Predicting in vivo oxygen consumption rate from ETS activity and bisubstrate enzyme kinetics in cultured marine zooplankton

2016 ◽  
Vol 163 (7) ◽  
Author(s):  
N. Osma ◽  
I. Fernández-Urruzola ◽  
M. Gómez ◽  
S. Montesdeoca-Esponda ◽  
T. T. Packard
Keyword(s):  
Oxygen Consumption ◽  
Enzyme Kinetics ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Ets Activity ◽  
Marine Zooplankton

scholarly journals Diffusion and Monod kinetics model to determine in vivo human corneal oxygen-consumption rate during soft contact lens wear

2015 ◽  
Vol 8 (1) ◽  
pp. 12-18 ◽  
Author(s):  
Luis F. Del Castillo ◽  
Ana R. Ferreira da Silva ◽  
Saul I. Hernández ◽  
M. Aguilella ◽  
Andreu Andrio ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Contact Lens ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Monod Kinetics ◽  
Soft Contact ◽  
Contact Lens Wear ◽  
Soft Contact Lens ◽  
Lens Wear

scholarly journals Ceria nanoparticles ameliorate renal fibrosis by modulating the balance between oxidative phosphorylation and aerobic glycolysis

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Mengling Wang ◽  
Feng Zeng ◽  
Fengling Ning ◽  
Yinhang Wang ◽  
Shilin Zhou ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Renal Fibrosis ◽  
Consumption Rate ◽  
Metabolic Reprogramming ◽  
Ceria Nanoparticles ◽  
Extracellular Acidification ◽  
Extracellular Acidification Rate

Abstract Background and aims Renal fibrosis is the common outcome in all progressive forms of chronic kidney disease. Unfortunately, the pathogenesis of renal fibrosis remains largely unexplored, among which metabolic reprogramming plays an extremely crucial role in the evolution of renal fibrosis. Ceria nanoparticles (CeNP-PEG) with strong ROS scavenging and anti-inflammatory activities have been applied for mitochondrial oxidative stress and inflammatory diseases. The present study aims to determine whether CeNP-PEG has therapeutic value for renal fibrosis. Methods The unilateral ureteral obstructive fibrosis model was used to assess the therapeutic effects in vivo. Transforming growth factor beta1-induced epithelial-to-mesenchymal transition in HK-2 cells was used as the in vitro cell model. The seahorse bioscience X96 extracellular flux analyzer was used to measure the oxygen consumption rate and extracellular acidification rate. Results In the present study, CeNP-PEG treatment significantly ameliorated renal fibrosis by increased E-cadherin protein expression, and decreased α-SMA, Vimentin and Fibronectin expression both in vitro and in vivo. Additionally, CeNP-PEG significantly reduced the ROS formation and improved the levels of mitochondrial ATP. The seahorse analyzer assay demonstrated that the extracellular acidification rate markedly decreased, whereas the oxygen consumption rate markedly increased, in the presence of CeNP-PEG. Furthermore, the mitochondrial membrane potential markedly enhanced, hexokinase 1 and hexokinase 2 expression significantly decreased after treatment with CeNP-PEG. Conclusions CeNP-PEG can block the dysregulated metabolic status and exert protective function on renal fibrosis. This may provide another therapeutic option for renal fibrosis. Graphical Abstract

Enhancement of both cardiac contraction and metabolism by a transient cooling of the lobster Panulirus japonicus

1997 ◽  
Vol 48 (8) ◽  
pp. 857 ◽  
Author(s):  
Taketeru Kuramoto ◽  
Masaki Tani
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Isolated Heart ◽  
Cold Treatment ◽  
Cardiac Contraction ◽  
Membrane Excitability ◽  
Cold Stimulation ◽  
Panulirus Japonicus

Contractile and metabolic activities of the isolated heart of Panulirus japonicus were recorded while thermal and hormonal stimuli were applied for 3–6 min. The heart beat was often augmented in amplitude with a fall in temperature from 20° to 17–15°C. With the augmented beat, a decrease of pO2 in the ventricle indicated an increase in oxygen consumption rate of the heart. When serotonin (0.1 µM) and the cold stimulation were applied at the same time, pO2 in the ventricle decreased, suggesting that the metabolic rate of the heart markedly rose. In contrast, octopamine (1 µM) raised the oxygen consumption rate of the heart only slightly. Octopamine greatly enhanced the membrane excitability and contraction force of the heart during cold treatment. A transient period of cooling in vivo may cause the pericardial organs to release octopamine and serotonin in P. japonicus. This may be relevant to the care of spiny lobsters during mariculture, capture, handling and transport.

scholarly journals Myricetin Exerts Anti-Obesity Effects through Upregulation of SIRT3 in Adipose Tissue

Nutrients ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1962 ◽  
Author(s):  
Seun Akindehin ◽  
Young-Suk Jung ◽  
Sang-Nam Kim ◽  
Yeon-Ho Son ◽  
Icksoo Lee ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Biologically Active ◽  
Beneficial Effects ◽  
Reduced Body ◽  
Sirt3 Expression

Myricetin is a biologically active natural polyphenol with beneficial effects on metabolic health. This study aimed to examine the effects of myricetin on the expression levels of genes involved in lipolysis and mitochondrial respiration in adipocytes and the anti-obesity potential of myricetin. The results indicated that myricetin reduced triglyceride (TG) content and increased mitochondrial content and oxygen consumption rate (OCR) in adipocytes in vitro. To determine anti-obesity effect of myricetin, C57BL6/J mice were fed a high-fat diet (HFD) for eight weeks and then treated with myricetin (10 mg/kg) for 2 weeks. The in vivo treatment of myricetin reduced body weight by 11%. Furthermore, it improved the glucose tolerance, and increased fatty acid consumption of HFD-fed mice. Myricetin treatment increased Sirt3 expression and reduced the acetylation of mitochondrial proteins in adipose tissue. Finally, the knockdown of Sirt3 in adipocytes reduced the myricetin-induced increase in mitochondrial oxygen consumption rate by about 27% compared to controls. Our results indicated that myricetin exerted anti-obesity effects through the upregulation of Sirt3 expression and mitochondrial metabolism in adipose tissue.

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