Kinetics and Performance of Biological Activated Carbon Reactor for Advanced Treatment of Textile Dye Wastewater

The kinetics and performance of a biological activated carbon (BAC) reactor were evaluated to validate the proposed kinetic model. The Freundlich adsorption capacity (Ka) and adsorption intensity constants (n) obtained from the batch experiments were 1.023 ± 0.134 (mg/g) (L/mg)1/n and 2.036 ± 0.785, respectively. The effective diffusivity (Ds) of the substrate within the activated carbon was determined by comparing the adsorption model value with the experimental data to find the best fit value (4.3 × 10–4 cm2/d). The batch tests revealed that the yield coefficient (Y) was 0.18 mg VSS/mg COD. Monod and Haldane kinetics were applied to fit the experimental data and determine the biokinetic constants, such as the maximum specific utilization rate (k), half-saturation constant (KS), inhibition constant (Ki), and biomass death rate coefficient (kd). The results revealed that the Haldane kinetics fit the experimental data better than the Monod kinetics. The values of k, KS, Ki, and kdwere 3.52 mg COD/mg VSS-d, 71.7 mg COD/L, 81.63 mg COD/L, and 4.9 × 10−3 1/d, respectively. The BAC reactor had a high COD removal efficiency of 94.45% at a steady state. The average influent color was found to be 62 ± 22 ADMI color units, and the color removal efficiency was 73‒100% (average 92.3 ± 10.2%). The removal efficiency for ammonium was 73.9 ± 24.4%, while the residual concentration of ammonium in the effluent was 1.91 ± 2.04 mg/L. The effluent quality from the BAC reactor could meet the discharge standard and satisfy the reuse requirements of textile dye wastewater.

Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates

Background The members of the genus Caldicellulosiruptor have the potential for future integration into a biorefinery system due to their capacity to generate hydrogen close to the theoretical limit of 4 mol H2/mol hexose, use a wide range of sugars and can grow on numerous lignocellulose hydrolysates. However, members of this genus are unable to survive in high sugar concentrations, limiting their ability to grow on more concentrated hydrolysates, thus impeding their industrial applicability. In this study five members of this genus, C.owensensis, C. kronotskyensis, C.bescii, C.acetigenus and C.kristjanssonii, were developed to tolerate higher sugar concentrations through an adaptive laboratory evolution (ALE) process. The developed mixed population C.owensensis CO80 was further studied and accompanied by the development of a kinetic model based on Monod kinetics to quantitatively compare it with the parental strain. Results Mixed populations of Caldicellulosiruptor tolerant to higher glucose concentrations were obtained with C.owensensis adapted to grow up to 80 g/L glucose; other strains in particular C. kristjanssonii demonstrated a greater restriction to adaptation. The C.owensensis CO80 mixed population was further studied and demonstrated the ability to grow in glucose concentrations up to 80 g/L glucose, but with reduced volumetric hydrogen productivities ($$Q_{{{\text{H}}_{2} }}$$ Q H 2 ) and incomplete sugar conversion at elevated glucose concentrations. In addition, the carbon yield decreased with elevated concentrations of glucose. The ability of the mixed population C.owensensis CO80 to grow in high glucose concentrations was further described with a kinetic growth model, which revealed that the critical sugar concentration of the cells increased fourfold when cultivated at higher concentrations. When co-cultured with the adapted C.saccharolyticus G5 mixed culture at a hydraulic retention time (HRT) of 20 h, C.owensensis constituted only 0.09–1.58% of the population in suspension. Conclusions The adaptation of members of the Caldicellulosiruptor genus to higher sugar concentrations established that the ability to develop improved strains via ALE is species dependent, with C.owensensis adapted to grow on 80 g/L, whereas C.kristjanssonii could only be adapted to 30 g/L glucose. Although C.owensensis CO80 was adapted to a higher sugar concentration, this mixed population demonstrated reduced $$Q_{{{\text{H}}_{2} }}$$ Q H 2 with elevated glucose concentrations. This would indicate that while ALE permits adaptation to elevated sugar concentrations, this approach does not result in improved fermentation performances at these higher sugar concentrations. Moreover, the observation that planktonic mixed culture of CO80 was outcompeted by an adapted C.saccharolyticus, when co-cultivated in continuous mode, indicates that the robustness of CO80 mixed culture should be improved for industrial application.

Biodegradation of acid red 3BN dye in sequential batch reactor: parameters and kinetics studies

Textile and dye industries generate wastewater which is considered as highly polluted and carcinogenic. Due to this, treatment of wastewater is required earlier to discharge or recycle. In the present studies, treatment of dye bearing water (DBW) has been explored. The treatment was performed using activated sludge (mixed culture) for aerobic process in sequential batch reactor (SBR). The fill volume (V F) and fill time (t F) variation in the treatment of DBW was taken place. The initial value of dye concentration, chemical oxygen demand (COD), sludge, and hydraulic retention time (HRT) were found to play important role in the treatment. At optimum condition (HRT = 2.5 d), the 86.84% COD reduction of 190 mg/L COD, and 92.33% dye reduction of 339 mg/L dye were achieved. These values are equal to overall 94.85% dye reduction of 500 mg/L, and 93.15% COD reduction of 380 mg/L. As a result, 500 mg/L dye was reduced to 26 mg/L, and 380 mg/L COD was reduced to 25 mg/L. The biodegradation fitted to Monod kinetics, for which kinetics parameter values of specific growth rate constant of biomass µ = 0.0047 h−1, yield coefficient (Y) = 1.059, and substrate utilization rate (q) = 0.0044 h−1 were evaluated at HRT = 2.5 d. The results show, this process can be applied to treat Acid Red 3BN Dye Water (AR3BNDW).

Reservoir Souring Prediction in Deepwater Reservoirs for Field Development Planning

A deep-water Field X with two major Reservoirs U and L discovered recently offshore Malaysia is on development for early production. The subsurface plan for the Field X includes water injection. But the presence of sulphate rich seawater can provide a favorable environment for souring activity to take place. This study evaluates the reservoir souring potential for the green Field X as a result of seawater flooding. Reservoir souring is the increase of the hydrogen sulfide (H2S) concentration in produced reservoir fluids. As hydrogen sulfide is a highly toxic and corrosive gas, the production of H2S has a huge impact on the safety, infrastructure and facilities of the field. Whether a reservoir is susceptible to souring is dependent on a variety of factors. Some of these include water injection flow rate, temperature of the reservoir, presence of bacterial nutrients and rock minerology. Effective prediction of biogenic reservoir souring using computer models is essential when undertaking major technical and economic decisions regarding field development. For H2S concentration calculation PETRONAS utilized in-house stand-alone modeling tool that considers physicochemical hydrodynamics of multiphase flow, heat transfer, substrate propagation and bacterial activity. The simulator looks at bacterial growth both in planktonic and sessile forms. Monod kinetics is applied for the growth of bacteria, leading to the consumption of sulphate and volatile fatty acids which in-turn is linked to H2S generation. Along with H2S propagation, H2S scavenging by rock and H2S partitioning between the various phases is also accounted for. The model can also deal with the effects of lift gas, reinjection of sour produced water, injection of biocide and nitrite. Since the Field X is a green field and historical production data is unavailable, the model is calibrated against the provided field development plan (FDP) data with sensitivity analysis. The simulation runs show that the H2S breakthrough occurs before the end of production. The amount of H2S produced indicates that the risk of reservoir souring associated with seawater injection in U and L Reservoirs of the Field X is high. It is recommended to evaluate different reservoir souring preventive measures in combination with mitigative options in terms of chance of success, risks, and cost (CAPEX/OPEX) in the context of the Field X development plan.

Analysis of Biodegradation and Microbial Growth in Groundwater System Using New the Homotopy Perturbation Method

In this paper, we drive the concentration of microbial growth in the groundwater system. This model is based on the system of non-linear differential equations. The system of equations is solved by using the new homotopy perturbation method. We followed toluene degradation and bacterial growth by measuring toluene and oxygen concentrations and by direct cell counts. And the total amount of toluene degraded by Pseudomonal putida F1 in the sediment columns increased with rising concentration of the source and flow rate. In contrast, the efficiency of toluene removal slowly decreases. The approximate analytical expression of this model, the concentration of toluene and bacteria also consideration of a metabolite concentration, the microbial growth of attached and suspended bacteria, depending on the simultaneous presence of toluene. Finally, oxygen and dual Monod kinetics are discussed. The analytical solutions are also compared with simulation results and satisfactory the agreement is noted.

Mathematical analysis of laboratory microbial experiments demonstrating deterministic chaotic dynamics

Presented is a system of four ordinary differential equations and a mathematical analysis of microbiological experiments in a four-component chemostat—nutrient n, rods r, cocci c, and predators p. The analysis is consistent with the conclusion that previous experiments produced features of deterministic chaotic and classical dynamics depending on dilution rate. The surrogate model incorporates as much experimental detail as possible, but necessarily contains unmeasured parameters. The objective is to understand better the differences between model simulations and experimental results in complex microbial populations. The key methodology for simulation of chaotic dynamics, consistent with the measured dilution rate and microbial volume averages, was to cause the preference of p for r vs. c to vary with the r and c concentrations, to make r more competitive for nutrient than c, and to recycle some dying p biomass, leading to a modified version of the Monod kinetics model. Our mathematical model demonstrated that the occurrence of chaotic dynamics requires a predator, p, preference for r versus c to increase significantly with increases in r and c populations. Also included is a discussion of several generalizations of the existing model and a possible involvement of the minimum energy dissipation principle. This principle appears fundamental to thermodynamic systems including living systems. Several new experiments are suggested.

Simulation of Methane Gas Production Process from Animal Waste in a Discontinuous Bioreactor

Due to the importance of environmental protection and the need to use new energy and alternative to conventional fuels, renewable energy has received much attention. Due to this necessity, a discontinuous bioreactor producing methane gas from animal waste has been modeled and simulated in this research. Monod kinetics was used to express the relationship between the growth rate of microorganisms and substrate concentration. The fourth-order Rong Kuta numerical method solved the substrate consumption and production of microorganisms and methane gas. The effect of the initial concentration of microorganisms on methane production has also been investigated. The initial concentrations of substrate and microorganisms are 74.51 g/L and 61.1 g/L, respectively. The results of this study showed that the mathematical model deviates about 53.8% from the laboratory data. According to the presented model, the amount of methane produced after 70 days is equal to 29.10 g/L. The decomposition rate of the substrate and methane gas production depends on the substrate's residence time. Increasing the initial concentration of microorganisms produces methane gas in less time. The amount of methane produced is independent of the initial concentration of microorganisms. The model presented in this study can predict the time required to perform the reaction, optimal bioreactor performance, design of relevant process equipment, and increase the scale of equipment, such as storage tank and proper control to produce high purity methane more volume. Suitable in bioreactors.

Characterization and Development of Osmotolerant Caldicellulosiruptor Strains Targeting Enhanced Hydrogen Production from Lignocellulosic Hydrolysates

Background The members of the genus Caldicellulosiruptor have the potential for future integration into a biorefinery system due to their capacity to generate hydrogen close to the theoretical limit of 4 mol H 2 /mol hexose, use a wide range of sugars and can grow on numerous lignocellulose hydrolysates. However, members of this genus are unable to survive in high osmolarity conditions, limiting their ability to grow on more concentrated hydrolysates, thus impeding their industrial applicability. In this study five members of this genus, C. owensensis , C. kronotskyensis , C. bescii, C. acetigenus and C. kristjanssonii , were developed to tolerate higher osmolarities through an adaptive laboratory evolution (ALE) process. The developed strain C. owensensis CO80 was further studied accompanied by the development of a kinetic model based on Monod kinetics. Results Osmotolerant strains of Caldicellulosiruptor were obtained with C. owensensis adapted to grow up to 80 g/l glucose; other strains in particular C. kristjanssonii demonstrated a greater restriction to adaptation. C. owensensis CO80 was further studied and demonstrated the ability to grow in glucose concentrations up to 80 g/l glucose but with reduced volumetric hydrogen productivities (Q H2 ) and incomplete sugar conversion at elevated glucose concentrations. In addition, the carbon yield decreased with elevated concentrations of glucose. The ability of C. owensensis CO80 to grow in high glucose concentrations was further described with a kinetic growth model, which revealed that the critical osmolarity of the cells increased fourfold when cultivated at higher osmolarity. When co-cultured with the osmotolerant strain C. saccharolyticus G5 at a hydraulic retention time (HRT) of 20h, C. owensensis constituted only 0.09-1.58% of the population in suspension.Conclusions The adaptation of members of the Caldicellulosiruptor genus to higher osmolarity established that the ability to develop improved strains via ALE is species dependent, with C. owensensis adapted to grow on 80 g/l, whereas C. kristjanssonii could only be adapted to 30 g/l glucose. Although, C. owensensis CO80 was adapted to a higher osmolarity medium, the strain demonstrated reduced Q H2 with elevated glucose concentrations. This would indicate that while ALE permits adaptation to elevated osmolarities, this approach does not result in improved fermentation performances at these higher osmolarities. Moreover, the observation that planktonic culture of CO80 was outcompeted by an osmotolerant strain of C. saccharolyticus, when co-cultivated in continuous mode, indicates that the robustness of strain CO80 should be improved for industrial application .