Expression of progesterone receptor protein in the ovine uterus during the estrous cycle: Effects of nutrition, arginine and FSH

2018 ◽  
Vol 108 ◽  
pp. 7-15 ◽  
Author(s):  
Anna T. Grazul-Bilska ◽  
Jiratti Thammasiri ◽  
Aree Kraisoon ◽  
Arshi Reyaz ◽  
Casie S. Bass ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Estrous Cycle ◽  
Receptor Protein ◽  
Ovine Uterus

scholarly journals Components of a platelet-activating factor-signaling loop are assembled in the ovine endometrium late in the estrous cycle

2004 ◽  
Vol 287 (2) ◽  
pp. E233-E240 ◽  
Author(s):  
O. Chami ◽  
G. Evans ◽  
C. O'Neill
Keyword(s):  
Estrous Cycle ◽  
Platelet Activating Factor ◽  
Endometrial Tissue ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Short Term ◽  
Prostaglandin F ◽  
Paf Receptor ◽  
Ovine Uterus ◽  
Circulating Levels

Pulsatile release of uterine prostaglandin F2α (PGF2α) induces luteolysis in ruminants. Exogenous PAF is well known to cause PGF2α release from the ovine uterus. This study examines whether the components of a PAF-signaling loop exist in sheep at the time luteolysis is normally initiated. Day 14 of the cycle was the first day the uterus responded to an infusion of PAF, inducing a significant short-term increase in circulating levels of the PGF2α metabolite. There was a significant increase of PAF concentration ( P < 0.001) in the endometrium and PAF release by tissue explants ( P < 0.001) from day 10 to day 16 of the cycle. Endometrial tissue PAF receptor mRNA expression was induced ( P < 0.01) by estradiol and progesterone treatment of animals, and transcripts were present between days 10 and 16 of the estrous cycle. Western analysis of endometrial tissue showed marked upregulation of PAF receptor protein expression from day 14 of the cycle, and immunolocalization studies showed that the receptor expression was predominantly around the endometrial glands. PAF:acetylhydrolase was primarily located within the lumen of the endometrial glands. The study shows that a PAF-signaling loop was assembled within the ovine endometrium at the time that PGF2α pulsatility was first observed.

scholarly journals Progesterone and Interferon Tau Regulate Hypoxia-Inducible Factors in the Endometrium of the Ovine Uterus

Endocrinology ◽  
2008 ◽  
Vol 149 (4) ◽  
pp. 1926-1934 ◽  
Author(s):  
Gwonhwa Song ◽  
Jinyoung Kim ◽  
Fuller W. Bazer ◽  
Thomas E. Spencer
Keyword(s):  
Estrous Cycle ◽  
Hypoxia Inducible Factor ◽  
Cell Types ◽  
Mrna Abundance ◽  
Mrna Levels ◽  
Uterine Receptivity ◽  
Blastocyst Development ◽  
Interferon Tau ◽  
Carboxy Terminal ◽  
Ovine Uterus

In ruminants, progesterone (P4) from the ovary and interferon tau (IFNT) from the elongating blastocyst regulate expression of genes in the endometrium that are hypothesized to be important for uterine receptivity and blastocyst development. These studies determined effects of the estrous cycle, pregnancy, P4, and IFNT on hypoxia-inducible factor (HIF) expression in the ovine uterus. HIF1A mRNA, HIF2A mRNA, and HIF2A protein were most abundant in the endometrial luminal and superficial glandular epithelia (LE and sGE, respectively) of the uterus and conceptus trophectoderm. During the estrous cycle, HIF1A and HIF2A mRNA levels were low to undetectable on d 10 in the endometrial LE/sGE, increased between d 10 and 14, and then declined on d 16. Both HIF1A and HIF2A mRNA were more abundant in the endometrial LE/sGE of pregnant ewes. However, HIF3A, HIF1B, HIF2B, and HIF3B mRNA abundance was low in most cell types of the endometria and conceptus. Treatment of ovariectomized ewes with P4 induced HIF1A and HIF2A in the endometrial LE/sGE, and intrauterine infusion of ovine IFNT further increased HIF2A in P4-treated ewes, but not in ewes treated with P4 and the antiprogestin ZK 136,317. HIF3A, HIF1B, HIF2B, and HIF3B mRNA abundance was not regulated by either P4 or IFNT. Two HIF-responsive genes, carboxy-terminal domain 2 and vascular endothelial growth factor A, were detected in both the endometrium and conceptus. These studies identified new P4-induced (HIF1A and HIF2A) and IFNT-stimulated (HIF2A) genes in the uterine LE/sGE, and implicate the HIF pathway in regulation of endometrial epithelial functions and angiogenesis, as well as peri-implantation blastocyst development.

scholarly journals Regulation of estrogen receptor-alpha expression in MCF-7 cells by taxol

2004 ◽  
Vol 180 (3) ◽  
pp. 487-496 ◽  
Author(s):  
MB Martin ◽  
SV Angeloni ◽  
P Garcia-Morales ◽  
PF Sholler ◽  
MD Castro-Galache ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Progesterone Receptor ◽  
Gene Transcription ◽  
Estrogen Receptor Alpha ◽  
Receptor Protein ◽  
Subsequent Increase ◽  
Receptor Alpha ◽  
Alpha Gene ◽  
Er Alpha ◽  
Mcf 7

Results presented in this study demonstrate that treatment of MCF-7 cells with taxol resulted in induction of estrogen receptor-alpha (ER alpha) gene transcription with a subsequent increase in ER alpha mRNA; this effect was promoter specific since taxol did not affect total transcription in MCF-7 cells and lacked an effect on transcription of the human acidic ribosomal phosphoprotein protein PO, progesterone receptor, and pS2 genes. In contrast to the increase in transcription of the ER alpha gene, taxol inhibited translation of the ER alpha mRNA. This effect is also transcript specific since taxol did not alter total protein synthesis and did not affect the concentration of progesterone receptor protein in the cell. The overall result of taxol treatment was to decrease the concentration of ER alpha protein in the MCF-7 cells. Evidence is presented that the effects of taxol on ER alpha gene transcription may be mediated through the induction of p53.

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