Efficient follicular wave synchronization using a progesterone-releasing intravaginal device (PRIDΔ) in Camelus dromedarius

2018 ◽  
Vol 118 ◽  
pp. 203-211 ◽  
Author(s):  
Ayman Abdel-Aziz Swelum ◽  
Islam M. Saadeldin ◽  
Abdullah Moumen ◽  
Hani Ba-Awadh ◽  
Abdullah N. Alowaimer
Keyword(s):  
Camelus Dromedarius ◽  
Follicular Wave ◽  
Intravaginal Device

scholarly journals Studies on the Oestrous Cycle of Camel (Camelus dromedarius)

1999 ◽  
Vol 4 (1) ◽  
pp. 43
Author(s):  
M.M. Alfuraiji
Keyword(s):  
Corpus Luteum ◽  
Oestrous Cycle ◽  
Camelus Dromedarius ◽  
Natural Cycle ◽  
Blood Samples ◽  
Induced Ovulation ◽  
Follicular Wave ◽  
Wave Cycle ◽  
Arabian Camel

The aim of this study was to investigate the oestrous cycle of the Arabian camel. Eight female camels (Majaheem) were used. Daily blood samples were collected for 60 days to measure Oestradiol - 178 (E2) and progesterone (P4) levels using radioimmunoassays. The duration of the follicular wave cycle ranged from 25-30 days and the length of oestrous from 5-7 days. P, levels were low (<3.2 nmol/l) throughout the cycle, while E2 levels were high during oestrous. The corpus luteum was therefore not present during the natural cycle suggesting that Arabian camels experienced induced ovulation.

4 EFFECT OF A PROLONGED AROMATASE INHIBITOR TREATMENT ON PRE-OVULATORY OVARIAN FOLLICLES IN CATTLE

2012 ◽  
Vol 24 (1) ◽  
pp. 113 ◽  
Author(s):  
J. Yapura ◽  
J. Singh ◽  
R. J. Mapletoft ◽  
R. Pierson ◽  
D. Rogan ◽  
...  
Keyword(s):  
Aromatase Inhibitor ◽  
Corpus Luteum ◽  
Repeated Measures ◽  
Slow Release ◽  
The Body ◽  
Maximum Diameter ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Letrozole Treatment ◽  
Intravaginal Device

Letrozole, a non-steroidal aromatase inhibitor, prevents the body from producing its own oestrogen. The potential use of this compound for herd synchronization is supported by previous studies in which letrozole treatment increased mean plasma LH concentrations, prolonged the period of dominance of the extant dominant follicle and delayed emergence of the next follicular wave. Heifers given a 3-day regimen of letrozole exhibited greater corpus luteum diameter indicative of a luteotrophic effect. The objective of the present study was to test the hypothesis that letrozole treatment during the development of the preovulatory follicular wave will delay ovulation. Post-pubertal beef heifers were given 2 luteolytic doses of PGF (12 h apart) and monitored by ultrasonography for ovulation. Ovarian follicular wave emergence was synchronized by ultrasound-guided transvaginal follicular ablation 5 to 8 days after PGF-induced ovulation (Day –1 = follicular ablation, Day 0 = wave emergence) and a luteolytic dose of PGF was given 60 and 72 h later. On Day 1, heifers were divided randomly into 2 groups (n = 15/group) and given an intravaginal device containing 1 g of letrozole or a blank device (control). The intravaginal devices were removed on Day 7, or at the time of ovulation, whichever occurred first. The ovaries were monitored by ultrasonography and a blood sample was collected daily from day of ablation to 12 days post-ovulation. Single point measurements were analysed by t-tests and serial data were analysed by analysis of variance for repeated measures. Multiple contrasts were made by Tukey's test. The interval from placement of the intravaginal device to ovulation was longer in letrozole-treated animals (6.1 ± 0.25 vs 5.1 ± 0.26 days, P < 0.01). Compared with controls, the day-to-day diameter profile of the dominant follicle of the ovulatory wave was larger (P < 0.05) and the maximum diameter greater (14.6 ± 0.51 vs 12.4 ± 0.53 mm; P < 0.01) in letrozole-treated heifers. The diameter profile of the corpus luteum formed post-letrozole treatment did not differ between groups; however, plasma P4 concentrations were higher (P < 0.01) in heifers treated with letrozole. In summary, a slow-release intravaginal letrozole device delayed ovulation by 24 h and induced the formation of a corpus luteum that secreted higher levels of progesterone. A slow-release intravaginal letrozole device may become useful for the development of an aromatase inhibitor-based protocol to control ovulation for herd synchronization and to enhance fertility by increasing circulating progesterone concentrations during the first 7 days post-AI or embryo transfer in cattle. Supported by the Natural Sciences and Engineering Research Council of Canada and Bioniche Life Sciences Inc.

116 Differences between 5- and 6-day progestogen-based oestrus induction protocol in Saanen and Toggenburg goats

2021 ◽  
Vol 33 (2) ◽  
pp. 166
Author(s):  
J. B. S. Pinheiro ◽  
J. M. G. Souza-Fabjan ◽  
L. F. L. Correia ◽  
M. E. F. Oliveira ◽  
J. F. Fonseca
Keyword(s):  
Animal Health ◽  
Hormonal Treatment ◽  
Induction Treatment ◽  
Device Removal ◽  
Short Term ◽  
Nonbreeding Season ◽  
Follicular Wave ◽  
Minas Gerais State ◽  
Oestrus Induction ◽  
Intravaginal Device

Hormonal oestrus induction treatment is an important tool for the success of reproductive biotechniques in anoestrus goats. In the nonbreeding season, oestrus induction can be performed by the use of several protocols, including those varying in their duration. This study aimed to compare short-term (for either 5 or 6 days) progestogen-based oestrus synchronization protocols in dairy [Toggenburg (n=12) and Saanen (n=12)] goats during the nonbreeding season. This study was performed in Minas Gerais State, Brazil (21°35′S and 43°15′W), with 12 nulliparous (12–14 months old) and 12 pluriparous (2–4 years old in final third of lactation) goats, all with body condition scores (BCS) of 3.0 to 3.5. They were equally assigned to both treatments according to their order of parturition, BCS, and breed. On a random day (Day 0), 24 goats received an intravaginal device of 60mg of medroxyprogesterone acetate (Progespon®, Zoetis) which remained for 5 (G5; n=12) or 6 (G6; n=12) days. On Day 5, goats from both groups received 30µg of cloprostenol intramuscularly (IM) (Prolise®, ARSA S.R.L.) and 200IU of equine chorionic gonadotrophin (eCG) IM (Novormon®, Schering Plough Animal Health). Both groups received cloprostenol and eCG on the same day (regardless of device removal) to ensure they were administered at the same follicular wave. Both device insertion and removal were performed in the morning. Ultrasound evaluations were performed every 12h from device removal to ovulation detection. Ovulations were considered as the average interval between the last observation of follicles and the first exam in which it was no longer seen. The normal distribution of variables was determined by Shapiro-Wilk test and homocedasticity by Levene’s test. All variables were not normally distributed and thus analysed by Mann–Whitney U test, with P&lt;0.05 considered significantly different. Values are presented in median±interquartile range. There was no difference between G5 and G6, respectively, in the interval to oestrus (48.0±0.0 vs. 48.0±12.0h), interval from oestrus onset to ovulation (18.0±9.0 vs. 18.0±12.0h), interval from device removal to ovulation (66.0±18.0 vs. 66.0±24.0h), number of ovulated follicles (2.0±2.0 vs. 2.5±2.0), or mean diameter of ovulated follicles (6.6±1.6 vs. 6.6±1.4mm). All ovulations were detected in the morning. In conclusion, our data demonstrate that short-term hormonal treatment for 5 or 6 days is similarly efficient to induce synchronous oestrus in Saanen and Toggenburg goats. This research was supported by EMBRAPA (Project 20.19.01.004.00.03.001) and CNPq (314952/2018-7).

Ultrasonography and hormone profiles of persistent ovarian follicles (cysts) induced with low doses of progesterone in cattle

Reproduction ◽  
2000 ◽  
pp. 361-366 ◽  
Author(s):  
KM Noble ◽  
JE Tebble ◽  
D Harvey ◽  
H Dobson
Keyword(s):  
Ovarian Follicles ◽  
Oestrous Cycle ◽  
Blood Collection ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Follicular Wave ◽  
Low Concentrations ◽  
Group 2 ◽  
Intravaginal Device ◽  
Group 1

The aims of this study were to expose dominant ovarian follicles at the end of the oestrous cycle to low progesterone concentrations similar to those that occur during stress, and to examine the effect of a subsequent small increase in progesterone 10 days later. Half a progesterone releasing intravaginal device (0.5 PRID) was administered to 13 heifers from day 15 of the oestrous cycle. In group 1 (n = 7), one 0.5 PRID remained in place until day 40 or until each heifer ovulated. In group 2 (n = 6), the first 0.5 PRID was removed on day 28, and replaced immediately with a second 0.5 PRID. Ultra-sonography and blood collection (10 ml) were conducted each day for 26 days from day 14 and then on alternate days. The largest follicle that emerged during the first 5 days after insertion of the initial 0.5 PRID remained > 10 mm in diameter for 15.3 +/- 1.7 and 11.6 +/- 0.4 days in groups 1 and 2, respectively. This period of dominance, during which no other follicles emerged, was closely correlated with the duration of plasma oestradiol concentrations exceeding 10 pg ml(-1). In four heifers from group 1, the persistent follicle ovulated between days 30 and 37 (sub-group 1a; 0.5 PRID expelled). In three heifers from sub-group 1b (0.5 PRID retained), the dominant follicle secreted oestradiol for 17 +/- 5 days but remained detectable by ultrasonography for a total of 33 +/- 8 days (range 26-52 days). Monitoring continued beyond day 40 in these animals. In group 2, the new 0.5 PRID inserted on day 28 resulted in an increase in plasma progesterone concentration of 0.9 +/- 0.3 ng ml(-1). Simultaneously, oestradiol decreased by 10.1 +/- 3.3 pg ml(-1), and a new follicular wave emerged 5-7 days later. In conclusion, exposure to very low concentrations of progesterone produced persistent follicles that secreted oestradiol for 17 days. This oestradiol production could be disrupted by a second increase of 0.9 ng ml(-1) in peripheral progesterone concentration. In the absence of the second progesterone treatment, some of the persistent follicles remained detectable by ultrasonography for up to 52 days, despite cessation of oestradiol secretion.

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