Studying the Liver Function in Male Neonates of Rats Born to Sertraline-Treated Mothers

<p>Sertraline is an antidepressant which has toxic effects on the liver. This study was conducted to evaluate the effect of Sertraline administration in pregnancy on liver function of male neonates of rats. Twenty-five pregnant female Wistar rats were divided into 4 groups of 5.<strong> </strong>The control group did not receive any drug treatments, but experimental (Exp) groups 1, 2 and 3 received 5, 10 and 20 mg/kg Sertraline as gavage throughout the pregnancy, respectively. Twenty-two days after birth, male rats were divided into 4 groups of 10 based on the previous division and after weighing, by taking blood directly from the heart, serum levels of Alanine transaminase (Alt), Aspartate transaminase (AST), Alkaline phosphatase (Alp), Albumin (Alb), Total protein (TP), and Bilirubin (Bili) were measured and the liver tissue was also analyzed histopathologically after weighing. In Exp groups, a significant decrease in body weight, TP and Alb serum levels were observed compared to the control group (p&lt;0.05). In Exp group 3, a significant decrease in liver weight was observed compared to the control group (p˂0.05). In Exp groups 2 and 3, a significant increase in serum levels of Alp, Alt and Bili in was observed compared to the control group (p˂0.05). A significant increase in AST serum level was observed in Exp groups compared to the control group (p˂0.05). Liver tissue destruction was observed in all 3 Exp groups. The administration of Sertraline in pregnant female rats causes liver damage and increases liver enzymes and blood biochemical parameters in their male offspring.</p>

PREGNANCY AND BREAST CANCER: A CASE REPORT

During pregnancy cancer is rare, but in pregnant female breast cancer is the second most common cancer. Pregnancy associated breast cancer (PABC) is defined as breast cancer that is discovered during pregnancy or within one year of delivery. The present case is reported to emphasize the importance of early recognition of this condition. A 28-year-old antenatal female, who presented with palpable breast mass. Keywords: PABC.

A COMPARATIVE STUDY OF THE ASSOCIATION OF SERUM FERRITIN LEVEL WITH GESTATIONAL DIABETES MELLITUS IN THE DEPARTMENT OF OBSTETRICS AND GYNAECOLOGY AT SMS MEDICAL COLLEGE & ATTACHED GROUP OF HOSPITALS, JAIPUR

Background: The present study aimed to study the association of Serum Ferritin level with gestational diabetes mellitus Methods: All Pregnant Females in Gestational age of 16 to 32 weeks presenting to the Ante natal clinic of Department of Obstetrics and Gynaecology, SMS Medical College, Jaipur. Based on Oral Glucose tolerance test, study participants were divided into two groups, Pregnant female with Gestational Diabetes (GDM group) and Pregnant female without Gestational Diabetes (Controls) Results: Mean ferritin level was significantly higher in GDM group (33.51 ng/ml)) as compared to control group (24.97 ng/ml). This difference in mean ferritin level among the two groups was found to be statistically significant (p<0.001). This indicates that higher ferritin level is significantly associated with risk of GDM. Conclusion: Serum ferritin in this study had a significant correlation with development of GDM. High ferritin range was found in females with GDM. Routine use of monitoring of serum ferritin levels, during the antenatal visit in the mid trimester should be carried out, for early prediction of developing GDM. Keywords: GDM, Serum ferrtin, Metabolic conditions.

Co-factor Influences the Severity of Zika Virus-Associated Microcephaly

Microcephaly has been regarded the most remarkable consequence of the Zika virus (ZIKV) epidemic in Brazil 2015. It remains to be determined whether there are factors that contribute to the degree of brain lesion associated with ZIKV infection during pregnancy. Previous studies showed that socioeconomic conditions correlate with ZIKV-associated microcephaly. Certain nutritional deficits display the potential to interfere in the mechanistic target of rapamycin (mTOR) signaling, which plays a major role in the pathophysiology of ZIKV-associated microcephaly. We hypothesize that a nutritional or environmental co-factor that interferes in mTOR signaling correlates with ZIKV-associated birth defects. To assess this hypothesis, we plan to: 1) develop a mouse model of ZIKV-associated microcephaly through intravenous injection of ZIKV and rapamycin for a straightforward interference on mTOR receptor; 2) determine in the experimental model and in cases of ZIKV-associated microcephaly the epigenetic signature (DNA methylation pattern) in neurons and muscle cells harvested by biopsy, and in hematopoietic and mesenchymal stem cells sorted from blood; 3) analyze through mass spectrometry in serum of pregnant female mice submitted to ZIKV and rapamycin injection and in serum of mothers of children with ZIKV-associated microcephaly the metabolomic pattern of cholesterol (a nutritional status marker), vitamin A and its metabolite retinoic acid, folate, and other metabolites related to these three nutritional factors; 4) check whether pregnant female mice submitted to intravenous injection of ZIKV and feed with a deficient diet of the most likely co-factor found in this study give birth to microcephalic mice with features that mimic clinical cases. In summary, our general objective is to develop an experimental model that mimics ZIKV-associated microcephaly cases and to find a co-factor involved in the microcephaly outbreak in Brazil 2015.

AN ASSESSMENT INTO THE DIAGNOSTIC VALUE OF ULTRASOUND IN THE FIRST TRIMESTER OF PREGNANCY BLEEDING

Background: One of the most prevalent obstetric issues is vaginal bleeding in the first trimester. It's also one of the most prevalent reasons for emergency admissions, as well as a reason for ultra-sound evaluation in the first trimester. In the first trimester, over a quarter of all pregnant female experience bleeding. Aims and objective: The purpose of this research was to determine the diagnostic value of ultra-sonography in first-trimester haemorrhage. Materials and method: All pregnant female who experienced per vaginal bleeding during the first trimester were included in this research. A semi-structured questionnaire was used to assess all of the selected instances. To arrive at a clinical opinion, a full history and comprehensive clinical evaluation were undertaken, including general, systemic, per abdominal, and per vaginal evaluations. In all of the cases that were chosen, ultra-sonography was used. The results of the clinical evaluation and ultra-sonography were documented. Results: On clinical evaluation, 164 cases of threatened abortion were identified, whereas ultra-sonography verified 102 cases of impending abortion. In 62 cases, there was a discrepancy in opinion. Complete abortion had a 16 percent inconsistancy, while incomplete abortion had a 4 percent inconsistancy. In 20 cases of Blighted ovum, there was a inconsistancy. Out of 214 occurrences of first trimester bleeding, abortion was diagnosed in 200 cases (93.46 percent), ectopic pregnancy in 10 cases (4.67 percent), and hydatiform mole in four cases (1.87 percent). Clinical opinion had a 100% sensitivity in diagnosing a viable intrauterine pregnancy, but only a 44.6 percent specificity. Clinical opinion has a poor statistical correlation in diagnosing nonviable pregnancies, with a sensitivity of 39%. Conclusion: Ultra-sonography has thus been established as a critical diagnostic tool in obstetrics. It is a readily available diagnostic tool that aids in the early detection of problems associated with first-trimester haemorrhage. It was established in the aforementioned research that it had an essential role in the opinion of first trimester haemorrhage. Key Word: first trimester bleeding, ultasonography, diagnostic importance

Study of sympathetic and parasympathetic control of haemodynamics in mid pregnancy by cardiac autonomic neuropathy analyzer

Pregnancy is associated with substantial changes in cardiovascular system. The action of autonomic nervous system is essential for circulatory adaptations in pregnancy and nourishing growing fetus. The study was conducted to assess the significance of autonomic function tests amongst women of mid pregnancy and non pregnant women. A comparative study was caried out amongst pregnant and non pregnant women in Department of Physiology. A total of 60 women (30 pregnant as study group and 30 non pregnant healthy women as control group) aged between 18- 30 years were included in study. The results indicated that there was statistically significant change in resting heart and highly statistically significant change to deep breathing in mid pregnancy reflecting higher parasympathetic activity in study group (pregnant female) as compared to control group (non pregnant female) while sympathetic activity assessed by isometric hand grip test didn’t show any statistically significant change among pregnant as compared to non pregnant. Our study showed role of parasympathetic activity in controlling haemodynamics in mid pregnancy and it may help to return the arterial pressure to non pregnant level by causing haemodilution, although when the increase in activity is excessive, hypertension may ensue.

Female reproductive organs procurement v1

Description of procurement of non-pregnant female reproductive tract from deceased donor for HuBMAP.