Acute effects of estradiol-17β on plasma volume and uterine cell proliferation in sheep

2021 ◽  
Vol 176 ◽  
pp. 12-17
Author(s):  
M.A. Vasquez-Hidalgo ◽  
K. Kelany ◽  
A.T. Grazul-Bilska ◽  
M. Bauer ◽  
K.C. Swanson ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Plasma Volume ◽  
Acute Effects ◽  
Estradiol 17Β

scholarly journals Conjugated linoleic acids attenuate FSH- and IGF1-stimulated cell proliferation; IGF1, GATA4, and aromatase expression; and estradiol-17β production in buffalo granulosa cells involving PPARγ, PTEN, and PI3K/Akt

Reproduction ◽  
2012 ◽  
Vol 144 (3) ◽  
pp. 373-383 ◽  
Author(s):  
Isha Sharma ◽  
Dheer Singh
Keyword(s):  
Cell Proliferation ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Function ◽  
Endocrine System ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Beneficial Effects ◽  
Tensin Homolog ◽  
Estradiol 17Β

Conjugated linoleic acid (CLA) has drawn much interest in last two decades in the area ranging from anticancer activity to obesity. A number of research papers have been published recently with regard to CLA's additional biological functions as reproductive benefits. However, not much is known how this mixture of isomeric compounds mediates its beneficial effects particularly on fertility. In this study, we demonstrated the cross talk between downstream signaling of CLA and important hormone regulators of endocrine system, i.e. FSH and IGF1, on buffalo granulosa cell function (proliferation and steroidogenesis). Experiments were performed in primary serum-free buffalo granulosa cell culture, where cells were incubated with CLA in combination with FSH (25 ng/ml) and IGF1 (50 ng/ml). Results showed that 10 μM CLA inhibits FSH- and IGF1-induced granulosa cell proliferation; aromatase,GATA4, andIGF1mRNA; and estradiol-17β production. Western blot analysis of total cell lysates revealed that CLA intervenes the IGF1 signaling by decreasing p-Akt. In addition, CLA was found to upregulate peroxisome proliferator-activated receptor-gamma (PPARG) and phosphatase and tensin homolog (PTEN) level in granulosa cells. Further study using PPARG- and PTEN-specific inhibitors supports the potential role of CLA in granulosa cell proliferation and steroidogenesis involving PPARG, PTEN, and PI3K/Akt pathway.

Effects of Estradiol-17β on Germ Cell Proliferation andDMYExpression during Early Sexual Differentiation of the Medaka Oryzias latipes

2005 ◽  
Vol 22 (7) ◽  
pp. 791-796 ◽  
Author(s):  
Aya Suzuki ◽  
Masatoshi Nakamoto ◽  
Yuko Kato ◽  
Naoki Shibata
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Sexual Differentiation ◽  
Oryzias Latipes ◽  
Estradiol 17Β ◽  
Germ Cell Proliferation

scholarly journals Estradiol 17β and Its Metabolites Stimulate Cell Proliferation and Antagonize Ascorbic Acid-Suppressed Cell Proliferation in Human Ovarian Cancer Cells

2013 ◽  
Vol 21 (1) ◽  
pp. 102-111 ◽  
Author(s):  
Hui-Hui Li ◽  
Ying-Jie Zhao ◽  
Yan Li ◽  
Cai-Feng Dai ◽  
Sheikh O. Jobe ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Ascorbic Acid ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Stimulate Cell ◽  
Estradiol 17Β ◽  
Stimulate Cell Proliferation

Impacts of linseed meal and estradiol-17β on cellularity, angiogenic and vasoactive factor mRNA expression, and vascularity of the uterus in ovariectomized ewes

2012 ◽  
Vol 92 (3) ◽  
pp. 297-306 ◽  
Author(s):  
B. R. Ilse ◽  
M. R. O'Neil ◽  
G. P. Lardy ◽  
L. P. Reynolds ◽  
K. A. Vonnahme
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Mrna Expression ◽  
Growth Factor Receptor ◽  
Percentage Change ◽  
Angiogenic Factor ◽  
Percentage Increase ◽  
Linseed Meal ◽  
Estrogenic Potential ◽  
Estradiol 17Β

Ilse, B. R., O'Neil, M. R., Lardy, G. P., Reynolds, L. P. and Vonnahme, K. A. 2012. Impacts of linseed meal and estradiol-17β on cellularity, angiogenic and vasoactive factor mRNA expression, and vascularity of the uterus in ovariectomized ewes. Can. J. Anim. Sci. 92: 297–306. The objective of the current study was to determine the estrogenic potential of the phytoestrogen secoisolariciresinol diglycoside (SDG) found in linseed meal (LSM) on uterine cell proliferation, vascularity, and angiogenic factor mRNA expression. Ovariectomized ewes (n=48) were fed a diet containing 12.5% LSM for 0, 1, 7, or 14 d and implanted with estradiol-17β (E2) for 0, 6, or 24 h before tissue collection. There was an interaction of LSM×E2 on uterine mass (grams; P=0.03; percentage change; P<0.003). Uterine mass increased (P≤0.02) after 24 h of E2 exposure on days 1, 7, and 14 of LSM feeding, with the greatest mass occurring in ewes exposed to E2 for 24 h and 1 d LSM feeding. Regardless of days fed LSM, after 24 h of E2 exposure uterine mass was greatest. The greatest percentage increase in uterine mass occurred in ewes exposed to E2 for 24 h and fed 1 d of LSM. Cell proliferation within the uterine luminal epithelium was greatest (P<0.01) with 24 h of E2 exposure compared with 0 h and 6 h. When expressed as the percentage change in uterine cell proliferation, feeding LSM for 14 d negated these effects. Only length of E2 exposure impacted vascularity with capillary number density at 6 h of E2 exposure being greater (P=0.02) than at 24 h. While mRNA expression of several angiogenic factors was influenced by E2, there was a LSM×E2 interaction (P≤0.03) only on vascular endothelial growth factor receptor 2 and fibroblast growth factor receptor 2C. It appears that growth and angiogenesis of E2 sensitive tissues may be influenced by the duration of LSM feeding.

808 THE ACUTE EFFECTS OF POSTURE AND EXERCISE ON PLASMA VOLUME AMD TOTAL CHOLESTEROL

1990 ◽  
Vol 22 (2) ◽  
pp. S135
Author(s):  
S. E. Sutherland ◽  
J. Kelly ◽  
G. Street
Keyword(s):  
Total Cholesterol ◽  
Plasma Volume ◽  
Acute Effects

scholarly journals Acute effects of ingesting glucose solutions on blood and plasma volume

2008 ◽  
Vol 101 (10) ◽  
pp. 1503 ◽  
Author(s):  
Gethin H. Evans ◽  
Susan M. Shirreffs ◽  
Ronald J. Maughan
Keyword(s):  
Plasma Volume ◽  
Acute Effects

ACUTE EFFECTS OF EXERCISE ON PLASMA LIPIDS, NORADRENALINE LEVELS AND PLASMA VOLUME

1991 ◽  
Vol 18 (10) ◽  
pp. 697-701 ◽  
Author(s):  
H. Krum ◽  
E. L. Conway ◽  
L. G. Howes
Keyword(s):  
Plasma Volume ◽  
Plasma Lipids ◽  
Acute Effects ◽  
Noradrenaline Levels
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